Carbonic Anhydrase in Green Plants

Waygood, E. R.; Clendenning, K. A.

doi:10.1139/cjr50c-041

Cited by 37 publications

(19 citation statements)

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“…2 and 3 and mammalian tissues (9,22). A comparison of these data on zinc distribution, showing a predominance in the supernatant fraction, with the subcellular distribution of zinc-requiring enzymes (17,19,23,24,26,27) reveals a close parallel.…”

Section: Discussionmentioning

confidence: 99%

Subcellular Localization of Zinc and Calcium in Bean (Phaseolus vulgaris L.) Tissues

1972

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Two bean (Phaseolus vulgaris L.) cultivars differing in growth responses to zinc were examined for differences in uptake and subcellular localization of 0Zn during a 15-day growth period. The zinc-sensitive cultivar Sanilac showed initially a much higher rate of absorption, which declined after 24 hours. The zinc-tolerant cultivar Saginaw showed a slow but steady rate of absorption for 10 days. In roots as well as in stem callus tissues of both cultivars, three-fourths of the absorbed "5Zn was localized in the "cytoplasmic" supernatant fractions (containing ribosomes and vacuolar sap). Very little (less than 7%) "Zn was localized in the cell wall fraction. There was a much greater proportion of the absorbed "Zn localized in root mitochondria and nuclei of the zinc-sensitive Sanilac than in the zinc-tolerant Saginaw. Stem The intracellular localization of enzymes in plant tissues has received wide attention (2,4,6,8,11,15,16,18,20

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Section: Discussionmentioning

confidence: 99%

Subcellular Localization of Zinc and Calcium in Bean (Phaseolus vulgaris L.) Tissues

1972

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“…inhibition of CA in crude extracts from sugar beet by 1.0 X lO-2 M KCN; using a partially purified enzyme preparation from spinach-beet, Sibly and Wood (1951) obtained 65 per cent. inhibition with 1.0 X lO-2 M KCN but practically no inhibition with 1.0 X 10-3 M KCN; and Waygood and Clendenning (1950) 1.0 X I()-3M KCN, the percentage inhibition being greater at the same concentration of KCN for dialysed extracts. In crude extracts of tomato leaves, used in the work described below, 1.0 X lQ-3M KCN caused 50 per cent.…”

Section: Introductionmentioning

confidence: 90%

“…Carbonic anhydrase (CA) activity in plants has been reported by N eish (1939), Day and Franklin (1946), Bradfield (1947), Waygood and Clendenning ( 1950), and Sibly and Wood (1951). Keilin and Mann (1940) established that CA isolated from animal tissues was a zinc-protein enzyme.…”

Section: Introductionmentioning

confidence: 99%

Carbonic Anhydrase Activity in Plants in Relation to Zinc Content

Wood¹,

Sibly²

1952

Aust. Jnl. Of Bio. Sci.

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SummaryThroughout the life cycle of oat plants carbonic anhydrase activity, which is restricted to the non-chloroplast fraction of leaves, reaches a maximum value and then decreases. After appearance of deficiency symptoms carbonic anhydrase activity is less in zinc-deficient plants than in fully manured controls of the same age and is associated with a lower zinc content of the leaves.An experiment is described wherein uniform tomato plants w'ere grown in zinc-deficient water cultures. The need for using uniform ontogenetic material is discussed. With the onset of zinc deficiency symptoms, which are described, four different concentrations of zinc were added to solutions in which the plants were growing; in order to minimize growth changes plants were harvested at short time intervals after application and carbonic anhydrase activity and zinc and protein-N contents were measured at each harvest.Carbonic anhydrase activity showed highly significant positive linear correlation with both zinc and protein-N contents.Evidence is presented which indicates that in zinc-deficient plants carbonic anhydrase activity is less than in normal plants through blocking of metabolic reactions leading to formation of protein and not by absence of sufficient zinc to activate an apoenzyme.

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“…Plant carbonic anlhydrase is thought to be only wveaklx affected, if at all, by sulfonamides (5,9) although the literature shows s,ome variability (1,11,13,19,24). It has been found by some workers that free sulfhydryl groups are necessarv for expression of the plant enzyme or for its enhancement (1,23) but variability in this regard has ailso been reported (15,24).…”

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“…No survey has yet been made oni the presence or absence anid distribution of carbonic anhv,drase in mlarinie macroscopic algae. 2 Its activity has been reported in 9 bacteria (22), microscopic algae (15,21), higher plants (8,24), invertebrates (18), and vertebrates (17), anid probably is of funldamiiental inmportance wherever CO.. is transported or exchanged.…”

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confidence: 99%