Carbon source-dependent variation of acquired mutagen resistance of Moniliophthora perniciosa: Similarities in natural and artificial systems

Santos, Regineide Xavier; Melo, Sônia Cristina Oliveira; Cascardo, Júlio Cézar M.; Brendel, Martin; Pungartnik, Cristina

doi:10.1016/j.fgb.2008.02.005

Cited by 15 publications

(21 citation statements)

References 42 publications

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“…This suggests again that in M. perniciosa, like in other pathogens, carbon sources behave as signaling molecules in addition to their nutritional role (Oliver et al, 2002;Thevelein et al, 2005;Vanoni et al, 2005). Indeed, GLY was previously related to acquired resistance to paraquat and oxidative stress in M. perniciosa (Santos et al, 2008) and to maintenance of the monokaryotic life form of this fungus in vitro (Meinhardt et al, 2006).…”

Section: Discussionmentioning

confidence: 80%

“…Recently, in-depth studies of this fungus have been published: M. perniciosa was found to produce hormones and alter endogenous auxin and salicylic acid levels in infected cocoa leaves (Kilaru et al, 2007), as well as necrosis-inducing proteins during infection, i.e., Mp-NeP1 and Mp-NeP2, which are differentially secreted during the course of infection in planta ; Rincones et al (2008) found that genes expressed in the biotrophic phase of M. perniciosa are under catabolite and nitrogen repression. Glycerol seems to play several roles in M. perniciosa biology; Santos et al (2008) showed that M. perniciosa's resistance to stress depends on glycerol, and Pungartnik et al (2009) showed that small amounts of hydrogen peroxide in glycerol medium induce the formation of clamp connections in vitro and that basidiospores are the most resistant life form of this fungus when compared to mono-and dikaryotic cells.…”

Section: Introductionmentioning

confidence: 99%

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Carbon source-induced changes in the physiology of the cacao pathogen Moniliophthora perniciosa (Basidiomycetes) affect mycelial morphology and secretion of necrosis-inducing proteins

Alvim¹,

Mattos²,

Pirovani³

et al. 2009

Genet. Mol. Res.

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ABSTRACT. Quantitative and qualitative relationships were found between secreted proteins and their activity, and the hyphal morphology of Moniliophthora perniciosa, the causal agent of witches' broom disease in Theobroma cacao. This fungus was grown on fermentable and non-fermentable carbon sources; significant differences in mycelial morphology were observed and correlated with the carbon source. A biological assay performed with Nicotiana tabacum leaves revealed that the necrosis-related activity of extracellular fungal proteins also differed with carbon source. There were clear differences in the type and quantity of the secreted proteins. In addition, the expression of the cacao molecular chaperone BiP increased after treatment with secreted proteins, suggesting a physiological response to the fungus secretome. We suggest that the carbon source-dependent energy metabolism of M. perniciosa results in physiological alterations in protein expression and secretion; these may affect not only M. perniciosa growth, but also its ability to express pathogenicity proteins.

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Section: Discussionmentioning

confidence: 80%

Section: Introductionmentioning

confidence: 99%

Carbon source-induced changes in the physiology of the cacao pathogen Moniliophthora perniciosa (Basidiomycetes) affect mycelial morphology and secretion of necrosis-inducing proteins

Alvim¹,

Mattos²,

Pirovani³

et al. 2009

Genet. Mol. Res.

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“…Glycerol [GLY] as a sole carbon source is required to maintain the growing monokaryotic mycelia for more than a month after basidiospore germination, while growth in glucose [GLU] leads to a switch from mono-to dikaryotic (necrotrophic) growth phase within a few days (Meinhardt et al, 2006). As sole source of carbon GLY also confers dikaryotic cells with enhanced resistance to oxidative stress (Santos et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Reactive oxygen species and autophagy play a role in survival and differentiation of the phytopathogen Moniliophthora perniciosa

Pungartnik

Melo

Basso

et al. 2009

Fungal Genetics and Biology

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The hemibiotrophic basidiomycete Moniliophthora perniciosa causes "witches' broom disease" in cacao (Theobroma cacao). During plant infection, M. perniciosa changes from mono to dikaryotic life form, an event which could be triggered by changes in plant nutritional offer and plant defense molecules, i.e., from high to low content of glycerol and hydrogen peroxide. We have recently shown that in vitro glycerol induces oxidative stress resistance in dikaryotic M. perniciosa. In order to understand under which conditions in parasite-plant interaction M. perniciosa changes from intercellular monokaryotic to intracellular dikaryotic growth phase we studied the role of glycerol on mutagen-induced oxidative stress resistance of basidiospores and monokaryotic hyphae; we also studied the role of H(2)O(2) as a signaling molecule for in vitro dikaryotization and whether changes in nutritional offer by the plant could be compensated by inducible fungal autophagy. Mono-/dikaryotic glycerol or glucose-grown cells and basidiospores were exposed to the oxidative stress-inducing mutagens H(2)O(2) and Paraquat as well as to pre-dominantly DNA damaging 4-nitroquinoline-1-oxide and UVC irradiation. Basidiospores showed highest resistance to all treatments and glycerol-grown monokaryotic hyphae were more resistant than dikaryotic hyphae. Monokaryotic cells exposed to 1microM of H(2)O(2) in glycerol-media induced formation of clamp connections within 2 days while 1mM H(2)O(2) did not within a week in the same medium; no clamp connections were formed in H(2)O(2)-containing glucose media within a week. Lower concentrations of H(2)O(2) and glycerol, when occurring in parallel, are shown to be two signals for dikaryotization in vitro and may be also during the course of infection. Q-PCR studies of glycerol-grown dikaryotic cells exposed to oxidative stress (10mM H(2)O(2)) showed high expression of MpSOD2 and transient induction of ABC cytoplasmic membrane transporter gene MpYOR1 and autophagy-related gene MpATG8. Expression of a second ABC transporter gene MpSNQ2 was 14-fold induced after H(2)O(2) exposure in glucose as compared to glycerol-grown hyphae while MpYOR1 did not show strong variation of expression under similar conditions. Glucose-grown dikaryotic cells showed elevated expression of MpATG8, especially after exposure to H(2)O(2) and 4-nitroquinoline-1-oxide. During different stages preceding basidiocarp formation MpATG8 and the two catalase-encoding genes MpCTA1 and MpCTT1 were expressed continuously. We have compiled our results and literature data in a model graph, which compares the in vitro and in planta development and differentiation of M. perniciosa with the help of physiological and morphological landmarks.

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“…The autophagic pathway of M. perniciosa is not very well described, except for some molecular data concerning protein expression (Santos et al, 2008;Pungartnik et al, 2009), in which the putative MpAtg8p is shown to be expressed during all phases of fungal growth with its induction related to oxidative stress. Therefore, the construction of a 3-D model of MpAtg8p permits the prediction of cellular function as well as provides a better understanding of domains present in the formed structure.…”

Section: Alignment Template and Construction Of The 3-d Modelmentioning

confidence: 99%

“…Glucose seems to trigger the expression of MpATG8, especially after mutagen-induced damage to cellular macromolecules. Also, MpATG8 is continuously expressed during the different stages preceding basidiocarp formation and during later steps of fungal differentiation (Santos et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

In silico modeling of the Moniliophthora perniciosa Atg8 protein

Pereira¹,

Cardoso²,

Brendel³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. Autophagy is defined as an intracellular system of lysosomal degradation in eukaryotic cells, and the genes involved in this process are conserved from yeast to humans. Among these genes, ATG8 encodes a ubiquitin-like protein that is conjugated to a phosphatidylethanolamine (PE) membrane by the ubiquitination system. The Atg8p-PE complex is important in initiating the formation of the autophagosome and thus plays a critical role in autophagy. In silico modeling of Atg8p of Moniliophthora perniciosa revealed its three-dimensional structure and enabled comparison with its Saccharomyces cerevisiae homologue ScAtg8p. Some common and distinct features were observed between these two proteins, including the conservation of residues required to allow the interaction of α-helix1 with the ubiquitin core. However, the electrostatic potential surfaces of these helices differ, implying particular roles in selecting specific binding partners. The proposed structure was validated by the programs PROCHECK 3.4, ANOLEA, and QMEAN, which demonstrated 100% of amino acids located in favorable regions with low total energy. Our results showed that MpAtg8p contains the same functional domains (3 α-helices and 4 β-sheets) and is similar in structure as the ScAtg8p yeast. Both proteins have many conserved sequences in common, and therefore, their proposed three-dimensional models show similar configuration.

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Carbon source-dependent variation of acquired mutagen resistance of Moniliophthora perniciosa: Similarities in natural and artificial systems

Cited by 15 publications

References 42 publications

Carbon source-induced changes in the physiology of the cacao pathogen Moniliophthora perniciosa (Basidiomycetes) affect mycelial morphology and secretion of necrosis-inducing proteins

Carbon source-induced changes in the physiology of the cacao pathogen Moniliophthora perniciosa (Basidiomycetes) affect mycelial morphology and secretion of necrosis-inducing proteins

Reactive oxygen species and autophagy play a role in survival and differentiation of the phytopathogen Moniliophthora perniciosa

In silico modeling of the Moniliophthora perniciosa Atg8 protein

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