Carbon quantum dots‐embedded electrospun antimicrobial and fluorescent scaffold for reepithelialization in albino wistar rats

Abstract

A prosthetic scaffold development using fluorescent nanofiber is reported for an enhanced reepithelialization in wistar albino rats. In this study, a novel approach was followed to construct the biocompatible fluorescent nanofiber that will be helpful to monitor the tissue regeneration process. Here, a multifunctional carbon quantum dots (CQDs)‐embedded electrospun polyacrylonitrile (PAN) nanofiber was fabricated and characterized using standard laboratory techniques. The biodegradation ability was assessed by simulated body fluid thereby analyzing porosity and water absorption capacity of the material. The fluorescent scaffold was tested for cytotoxicity and antimicrobial activity using bacterial and fibroblast cells and fluorescent stability was analyzed by bioimaging of animal and bacterial cells. Tissue regeneration capability of the developed scaffold was evaluated using wistar albino rats. Unlike biomicking scaffolds, the CQDs‐embedded PAN‐based substrate has given dual support by enhancing reepithelialization without growth factors and acted as an antimicrobial agent to provide contamination free tissue regeneration. Scaffolds were examined by using histostaining techniques and scanning electron microscopy to observe the reepithelialization in the regenerated tissues. The novel approach for developing infection free soft tissue regeneration was found to be phenomenal in scaffold development.

Comparison Direct Synthesis of Hyaluronic Acid-Based Carbon Nanodots as Dual Active Targeting and Imaging of HeLa Cancer Cells

“…55 Other studies (ISO 10993-5) have identified the cell viability in the range 80−120%, referring to the low toxicity on cells. 56,57 The cytotoxicity of the sample was compared with that of the untreated control with a cell viability of 100%. The cytotoxicity enhancement data have been intensively explored in determining the 50% cytotoxic concentration, or commonly called CC 50 , of each of the varied CDs (Figure S3, Supporting Information).…”

Electrically conductive carbon‐based (bio)‐nanomaterials for cardiac tissue engineering

“…180,181 In addition to the materials mentioned above, carbon-based materials also have the magnificent potential to result in electroactivity. These include graphite, 182,183 graphene, [184][185][186] graphene oxide, 187,188 reduced graphene oxide, [189][190][191] carbon nanofibers, 60,[192][193][194] carbon nanotubes, 156,195,196 fullerene, 197,198 carbon quantum dots, [199][200][201] and nanodiamonds. [202][203][204] Particular mechanical, electrical, thermal, and optical properties bring about the opportunity for carbon-based nanomaterials to be involved in the field of tissue repair.…”

Carbon Dots as an Emergent Class of Antimicrobial Agents

“…CDs can also be embedded inside patches for wound dressing purposes or inside nanofibrous materials commonly used in biomedical devices for a slow release and long-term antibacterial protection [ 150 , 151 ]. For instance, Jian et al [ 152 ] showed that spermidine/dopamin-based CDs (SPM/DA-CDs) can be used for the functionalization of 2-hydroxyethyl methacrylate, a polymer film that constitutes the main component of contact lenses.…”