Carbon nanoparticle modified screen printed carbon electrode as a disposable electrochemical immunosensor strip for the detection of Japanese encephalitis virus

Abstract

The authors describe a disposable electrochemical immunosensor strip for the detection of the Japanese encephalitis virus (JEV). The assay is based on the use of a screen printed carbon electrode (SPCE) modified with carbon nanoparticles (CNPs) that were prepared from starch nanoparticles and deposited on the SPCE working electrode whose surface was functionalized with 3-aminopropyl triethoxysilane. Next, antibody of JEV was immobilized on the surfaces of the CNPs. The analytical performance of immunosensor strip was characterized using cyclic voltammetry (with hexacyanoferrate as the redox probe) and electrochemical impedance spectroscopy. The deposition of CNPs enhances the electron transfer kinetics and current intensity of the SPCE by 63% compared to an unmodified SPCE. Under optimized conditions, the calibration plot is linear within the 5-20 ng·mL −1 JEV concentration range, the limit of detection being 2 ng·mL −1 (at an S/N ratio of 3), and the assay time is 20 min. This immunosensor strip was successfully applied to the detection of JEV in human serum samples. It represents a cost-effective alternative to conventional diagnostic tests for JEV.

Electrochemical biosensors for pathogen detection

“…3a) (De Luna et al 2017;Mahshid et al 2016). A study by Chin et al found that nanostructuring of carbon electrodes with carbon nanoparticles enhanced the electron transfer kinetics and current intensity of the electrode by 63% for the detection of Japanese encephalitis virus (Chin et al 2017).…”

Functionalized TiO2 Nanotube-Based Electrochemical Biosensor for Rapid Detection of SARS-CoV-2

“…The functionality of the electrochemical biosensor can be further improved by nanostructuring the electrode as it increases the electrochemical reaction rate due to an increased electrode surface area to volume ratio, thereby increasing the electrode surface area to analyte fluid volume. In the work by Chin et al on the encephalitis virus, it was found that nanostructuring of carbon electrodes with carbon nanoparticles increased the current response by 63% due to an enhanced electron charge transfer kinetics [ 23 ]. Similarly, we have reported that Co functionalized TiO 2 nanotubes (Ni-TNTs) with higher surface-to-volume ratio can detect the biomarkers associated with tuberculosis [ 24 , 25 ].…”

Functionalized TiO2 nanotube-based Electrochemical Biosensor for Rapid Detection of SARS-CoV-2

“…The functionality of the electrochemical biosensor can be further improved by nanostructuring the electrode as it increases the electrochemical reaction rate due to an increased electrode surface area to volume ratio, thereby increasing the electrode surface area to analyte fluid volume. In the work by Chin et al on the encephalitis virus, it was found that nanostructuring of carbon electrodes with carbon nanoparticles increased the current response by 63% due to an enhanced electron charge transfer kinetics [23]. Similarly, we have reported that Co functionalized TiO2 nanotubes (Ni-TNTs) with higher surface-to-volume ratio can detect the biomarkers associated with tuberculosis [24] [25].…”