Carbon Nano‐onions for Imaging the Life Cycle of Drosophila Melanogaster

Abstract

Real-time X-ray or magnetic resonance imaging are known methods used for biomedical diagnosis. By the oral administration of barium meal, X-ray imaging can be extended for use in soft tissue imaging. The oral ingestion of a fluorescent probe is a new approach to imaging a living species. Here, water-soluble carbon nano-onions are introduced as a nontoxic, fluorescent reagent enabling Drosophila melanogaster (fruit flies) to be imaged alive. It is demonstrated that these water-soluble carbon nano-onions, synthesized from wood waste, colorfully image all the development phases of Drosophila melanogaster from its egg to adulthood. Oral ingestion of up to 4 ppm of soluble carbon nano-onions allows the optical fluorescence microscopy imaging of all the stages of the fruit fly life cycle without showing any toxic effects. The fluorescent Drosophila melanogaster excretes this fluorescing material upon the withdrawal of carbon nano-onions from its food.

Nanocarbon–{[Na10(PrW10O36)]2·130H2O} composite to detect toxic food coloring dyes at nanolevel

“…Synthesis of carbon nano-onion was made by following the established procedures (Dubey et al 2005;Ghosh et al 2011). …”

“…To do so we have used a new hybrid system made up with nanocarbon onions-polyoxometalate, CNO/Pr-W Ln-POM, composite and used it in detecting trace amount of these toxic dyes used in food, beverages, syrup and medicines. The unique spectral, optical and electronic properties of carbon nano-onion (CNO) (Ghosh et al 2011) and newly synthesized polyoxometalate (POM) have great potential in medicinal and industrial research with many applications in modern science and technology.…”

DNA–carbon nano onion aggregate: triangle, hexagon, six-petal flower to dead-end network

“…Finally, at the end these regular structures may be driven to more stable network structure with the creation of extended dead-end network structure losing normal characteristics of DNA. As wsCNO is shown to be dose-dependent non-toxic (Ghosh et al 2011;Saxena et al 2013Saxena et al , 2014 it will be challenging to explore the interaction of wsCNO with rouged DNA to control their unwanted proliferation in certain cell growth and preventing such viral DNA replication in well-known diseases.…”

“…The watersoluble CNOs were synthesized using previously reported methods (Ghosh et al 2011;Sonkar et al 2012). Water from a Millipore Milli-Q system (conductivity B0.1 lS cm -1 ) has been used.…”

“…The dose-related toxicity-non toxicity issue of the nano carbon used in such cell line imaging has not been addressed. Use of water-soluble carbon nano onion (wsCNO) in known amount is shown to image the entire life cycle of Drosophila melanogaster (Ghosh et al 2011). In addition, the dose-dependent non-toxic and toxic use of wsCNO has also been reported in imaging the life cycle and controlling the population of mosquito, respectively (Saxena et al 2013).…”