Carbon fibre-based microbiosensors for in vivo measurements of acetylcholine and choline

Schuvailo, Oleg; Dzyadevych, S. V.; El'skaya, A. V.; Gautier-Sauvigné, Sabine; Csöregi, Elisabeth; Cespuglio, Raymond; Солдаткин, А. П.

doi:10.1016/j.bios.2004.09.017

Cited by 61 publications

(36 citation statements)

References 39 publications

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“…Improved temporal resolution can be achieved using biosensors for monitoring H 2 O 2 (Ferapontova et al, 2001;Schuvailo et al, 2005;Wang et al, 2003). While several biosensors have been developed for monitoring H 2 O 2 in bulk solution, only two have been used for measurements of H 2 O 2 in ECF (Kulagina and Michael, 2003;Mao et al, 2002) and only one of these is implantable (Kulagina and Michael, 2003).…”

Section: Introductionmentioning

confidence: 99%

Development and characterization in vitro of a catalase-based biosensor for hydrogen peroxide monitoring

O'Brien¹,

Killoran²,

O’Neill³

et al. 2007

Biosensors and Bioelectronics

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There is increasing evidence that hydrogen peroxide (H 2 O 2 ) may act as a neuromodulator in the brain, as well as contributing to neurodegeneration in diseased states, such as Parkinson's disease. The ability to monitor changes in endogenous H 2 O 2 in vivo with high temporal resolution is essential in order to further elucidate the roles of H 2 O 2 in the central nervous system. Here, we describe the in vitro characterization of an implantable catalase-based H 2 O 2 biosensor. The biosensor comprises two amperometric electrodes, one with catalase immobilized on the surface and one without enzyme (blank). The analytical signal is then the difference between the two electrodes. The H 2 O 2 sensitivity of various designs was compared, and ranged from 0 to 56 ± 4 mA cm −2 M −1 . The most successful design incorporated a Nafion ® layer followed by a poly-o-phenylenediamine (PPD) polymer layer. Catalase was adsorbed onto the PPD layer and then cross-linked with glutaraldehyde. The ability of the biosensors to exclude interference from ascorbic acid, and other interference species found in vivo, was also tested. A variety of the catalase-based biosensor designs described here show promise for in vivo monitoring of endogenous H 2 O 2 in the brain.

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Section: Introductionmentioning

confidence: 99%

Development and characterization in vitro of a catalase-based biosensor for hydrogen peroxide monitoring

O'Brien¹,

Killoran²,

O’Neill³

et al. 2007

Biosensors and Bioelectronics

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“…Henriksen and 110 co-workers [15] also developed a recombinant SBP for crystallisation studies based 111 on previous cDNA work, in which they successfully refolded SBP from inclusion 112 bodies using a cocktail that included both oxidized and reduced glutathione [27]. 113 There have been several other recent examples of recombinantly expressed plant 114 peroxidases including Hushpulian and co-workers' [28] work on tobacco anionic 115 peroxidase. 116…”

Section: Sbp: Cloning and Expression 103mentioning

confidence: 99%

Horseradish and soybean peroxidases: comparable tools for alternative niches?

Ryan

Carolan

Ó’Fágáin

2006

Trends in Biotechnology

139

102

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Horseradish and soybean peroxidases (HRP and SBP, respectively) are useful 9 biotechnological tools. HRP is often termed the classical plant heme peroxidase, and 10 although it has been studied for decades our understanding has deepened since its 11 cloning and subsequent expression, which has enabled numerous mutational and 12 protein engineering studies. SBP, however, has been neglected until recently; despite 13 offering a real alternative to HRP that actually outperforms it in terms of stability. 14 SBP is now used in numerous biotechnological applications, including biosensors. 15Review of both is timely. This article summarises and discusses the main insights into 16 the structure and mechanism of HRP, with special emphasis on HRP mutagenesis, and 17 outlines its use in a variety of applications. It also reviews current knowledge and 18 applications to date of SBP, particularly biosensors. The final paragraphs speculate on 19 the future of plant heme-based peroxidases, with probable trends outlined and 20 explored. 21 22

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“…The uses of carbon fibre electrodes for biomedical applications are also well documented for the detection of: nitric oxide [25], lactate dehydrogenase [26], perphenazine [27], haemoglobin [28], ascorbate, catechol and indole [29], chloramphenicol [30] and uric acid [31,32] However the aforementioned detection of uric acid was performed in-vitro, using diluted sample and a large surface area sensor. Single fibre electrodes are commonly used for in-vivo monitoring for many other analytes, commonly used to measure analytes within the brain; acetylcholine and choline [33], dopamine [34][35][36], acetaminophen [37], nitric oxide [38] and glucose [39]. The challenge, and the emphasis of the current investigation, is to develop a processing strategy that allows the material to be harnessed in a way that can allow the rapid production of sensors that could be easily incorporated within a smart bandage and which can facilitate reagentless and periodic monitoring of urate directly within a range of biofluids.…”

Section: Nottingham Trent University August 2007mentioning

confidence: 99%

Carbon Fibre Composites: Integrated Electrochemical Sensors for Wound Management

Sharp

Forsythe

Davis

2008

The Journal of Biochemistry

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The applicability of employing a carbon fibre mesh as an electrochemical sensing substructure for assessing urate transformations within wound exudates is evaluated. Prototype sensor assemblies have been designed and their response characteristics towards uric acid and other common physiological components are detailed. Modification of the carbon fibre sensor through surface anodisation and the application of cellulose acetate permselective barriers have been shown to lead to optimized responses and much greater sensitivity (1440% increase) and specificity. These could enable the accurate periodic monitoring of uric acid in wound fluid. The performance characteristics of the composite sensors in whole blood, serum and blister fluid have been investigated. KeywordsCarbon fibre, smart bandage, urate, uric acid, wound infection 2 Sharp et al.

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Carbon fibre-based microbiosensors for in vivo measurements of acetylcholine and choline

Cited by 61 publications

References 39 publications

Development and characterization in vitro of a catalase-based biosensor for hydrogen peroxide monitoring

Development and characterization in vitro of a catalase-based biosensor for hydrogen peroxide monitoring

Horseradish and soybean peroxidases: comparable tools for alternative niches?

Carbon Fibre Composites: Integrated Electrochemical Sensors for Wound Management

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