Carbon Fibre Composites: Integrated Electrochemical Sensors for Wound Management

Sharp, Duncan; Forsythe, Stephen; Davis, James A.

doi:10.1093/jb/mvn045

Cited by 27 publications

(13 citation statements)

References 38 publications

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“…Recently there has been progress in discovering new infection markers for bacterial detection in wounds [11]. Monitoring the redox activity of pyocyanin and the uric acid level in wounds were demonstrated for the prediction of early wound infections [12,13]. Wound infection is the result of the systematic colonization of pathogens, and the associated virulence factors act as the weaponry of bacteria in damaging host tissues irreversibly.…”

Section: Electrochemicalmentioning

confidence: 99%

An electrochemical sensor concept for the detection of bacterial virulence factors from Staphylococcus aureus and Pseudomonas aeruginosa

Thet

Jenkins

2015

Electrochemistry Communications

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This work presents an electrochemical sensor concept for bacterial toxin detection using biomimetic lipid vesicles containing potassium ferricyanide. The toxin mediated release of redox couples from the vesicles was quantified by measuring the redox current on screen printed electrodes, and the detection limits to rhamnolipid, delta toxin and bacterial supernatants from clinical wound pathogens were examined. Overall detection limits of both redox and carboxyfluorescein containing vesicles were one order of magnitude lower than the cytotoxic dose of studied toxins to T lymphocytes.

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Section: Electrochemicalmentioning

confidence: 99%

An electrochemical sensor concept for the detection of bacterial virulence factors from Staphylococcus aureus and Pseudomonas aeruginosa

Thet

Jenkins

2015

Electrochemistry Communications

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“…Electrospun cellulose nitrate nanofibers when combined with conductive magnetic nanoparticles were shown to be useful in the sensitive detection of E coli bacteria [21]. A sensor that detects urate, a metabolite useful as a diagnostic marker of S. aureus and P. aeruginosa, utilized a carbon fiber sensor laminate in combination with cellulose acetate, which filtered out large proteins, and was partially successful when used with blood and blister fluid [22].…”

Section: Detection Of Cellular Markersmentioning

confidence: 99%

Nanocellulose-Based Biosensors: Design, Preparation, and Activity of Peptide-Linked Cotton Cellulose Nanocrystals Having Fluorimetric and Colorimetric Elastase Detection Sensitivity

Edwards¹,

Prevost²,

French³

et al. 2013

ENG

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Nanocrystalline cellulose is an amphiphilic, high surface area material that can be easily functionalized and is biocompatible and eco-friendly. It has been used singularly and in combination with other nanomaterials to optimize biosensor design. The attachment of peptides and proteins to nanocrystalline cellulose and their proven retention of activity provide a route to bioactive conjugates useful in designs for point of care biosensors. Elastase is a biomarker for a number of inflammatory diseases including chronic wounds, and its rapid sensitive detection with a facile approach to sensing is of interest. An increased interest in the use of elastase sensors for point of care diagnosis is resulting in a variety of approaches to elsastase sensors utilizing different detection technologies. Here elastase substrate peptide-celluose conjugates synthesized as colorimetric and fluorescent sensors on cotton cellulose nanocrystals are compared. The structure of the sensor peptide-nanocellulose crystals when modeled with computational crystal structure parameters demonstrates the spatio-stoichiometric features of the nanocrystalline surface that allows ligand to active site protease interacttion. An understanding of the structure/function relations of enzyme and conjugate substrate of the peptides covalently attached to nancellulose has implications for enhancing the biomolecular transducer. The potential applications of both fluorescent and colorimetric detection to markers like elastase using peptide cotton cellulose nanocrystals as a transducer surface to model point of care biosensors for protease detection are discussed.

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“…The electrodes were modified with the application of a cellulose acetate permselective barrier to avoid biofouling. The team reported a sensitivity range of 0-500 µM for a linear fit (R^2 = 0.97) [89]. RoyChoudhury and coworkers developed an enzymatic electrochemical sensor to detect uric acid changes in real-time by the immobolization of uricase and detecting the byproduct H2O2.…”

Section: Uric Acidmentioning

confidence: 99%

“…This could be accomplished by the utilization of a working electrode that has a potential of -0.5 to -1.4V (electroreduction) and 0.6 to 0.9V (electrooxidation) vs AG/AgCl [81]. However, these electrodes experience biological interferences such as L-arginine, sodium nitrate, sodium nitrite, oxygen, and hydrogen peroxide [89,90]. Biological interference is filtered out by the inclusion of a transition metal or a metalloprotein such as hemoglobin [81].…”

Section: Nitric Oxidementioning

confidence: 99%

Smart Wound Dressings for Diabetic Chronic Wounds

Gianino

Miller

Gilmore

2018

Preprint

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Given their severity and non-healing nature, diabetic chronic wounds are a significant concern to the 30.3 million Americans diagnosed with diabetes mellitus (2015). Peripheral arterial diseases, neuropathy, and infection contribute to the development of these wounds, which lead to an increased incidence of lower extremity amputations. Early recognition, debridement, offloading, and controlling infection are imperative for timely treatment. However, wound characterization and treatment are highly subjective and based largely on the experience of the treating clinician. Many wound dressings have been designed to address particular clinical presentations, but a prescriptive method is lacking for identifying the particular state of chronic, non-healing wounds. The authors suggest that recent developments in wound dressings and biosensing may allow for the quantitative, real-time representation of the wound environment, including exudate levels, pathogen concentrations, and tissue regeneration. Development of such sensing capability could enable more strategic, personalized care at the onset of ulceration and limit the infection leading to amputation. This review presents an overview of the pathophysiology of diabetic chronic wounds, a brief summary of biomaterial wound dressing treatment options, and biosensor development for biomarker sensing in the wound environment.

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Carbon Fibre Composites: Integrated Electrochemical Sensors for Wound Management

Cited by 27 publications

References 38 publications

An electrochemical sensor concept for the detection of bacterial virulence factors from Staphylococcus aureus and Pseudomonas aeruginosa

An electrochemical sensor concept for the detection of bacterial virulence factors from Staphylococcus aureus and Pseudomonas aeruginosa

Nanocellulose-Based Biosensors: Design, Preparation, and Activity of Peptide-Linked Cotton Cellulose Nanocrystals Having Fluorimetric and Colorimetric Elastase Detection Sensitivity

Smart Wound Dressings for Diabetic Chronic Wounds

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