Carbon Dots-Modified Nanoporous Membrane and Fe3O4@Au Magnet Nanocomposites-Based FRET Assay for Ultrasensitive Histamine Detection

Abstract

Histamine can be formed by enzymatic decarbonylation of histidine, which is an important indicator of seafood quality. A rapid and sensitive assay method is necessary for histamine monitoring. A fluorescence resonance energy transfer (FRET) assay system based on a carbon dot (CD)-modified nanoporous alumina membrane and Fe3O4@Au magnet nanocomposites has been developed for histamine detection in mackerel fish. CDs immobilized on nanoporous alumina membranes were used as donors, which provided a fluorescence sensing substrate for histamine detection. Fe3O4@Au magnet nanocomposites can not only act as acceptors, but also concentrate histamine from fish samples to increase detection sensitivity. Histamine was detected by the fluorescence signal changes of CDs capturing histamine by an immune reaction. The fluorescence signals of CDs were quenched by Fe3O4@Au magnet nanocomposites via the FRET mechanism. With an increase of histamine, the fluorescence intensity decreased. By recording fluorescence spectra and calculating intensity change, histamine concentration can be determined with a limit of detection (LOD) of 70 pM. This assay system can be successfully applied for histamine determination in mackerel fish to monitor the fish spoilage process in different storage conditions. It shows the potential applications of CDs-modified nanoporous alumina membranes and Fe3O4@Au magnet nanocomposites-based biosensors in the food safety area.

Light-Interacting iron-based nanomaterials for localized cancer detection and treatment

“…In most FRET studies, a FRET-based IBN complex was activated through the binding of FC to IBN or via the structural change of the FRET IBN complex, which took place without FC release from IBN. FIBN can contain FRET pairs consisting of a donor such as GFP, [123], Carbon dots, [124], FITC, [125], PFR, [126], and an acceptor such as CdTe QD, [123], Au [124,126], or iron oxide nanoparticle (IONP), [125]. When FC gets linked to IBN to form a FRET IBN complex, it yields a fluorescence decrease of the FC donor and the binding of FC to this complex can then be detected.…”

“…When FC gets linked to IBN to form a FRET IBN complex, it yields a fluorescence decrease of the FC donor and the binding of FC to this complex can then be detected. Hence, FRET can be used to detect the binding of a substance of interest such as sarcosine, [123], histamine, [124], thiol, [126], to IBN with a high sensitivity, i.e.…”

Recent advances in the application of Raman spectroscopy for fish quality and safety analysis

“…Although extensive laboratory procedures for histamine in fish have been developed over time, rapid and simplified methods are still needed for histamine concentration detection. Despite the fact that great progress has been made in histamine detection by fluorescence technique (Mao et al, 2019;Seto et al, 2010;Yoshitake et al, 2003), some challenges remain including the fussy pre-treatment and poor selectivity. To surmount the aforementioned disadvantages, molecular imprinting is exploited for the efficient separation and enrichment of target analytes from complex matrices.…”

Zero-to-Two Nanoarchitectonics: Fabrication of Two-Dimensional Materials from Zero-Dimensional Fullerene

“…Functional materials in huge varieties have been prepared and fabricated through efforts in organic synthesis [ 7 , 8 , 9 , 10 ], polymer chemistry [ 11 , 12 , 13 ], coordination chemistry [ 14 , 15 , 16 , 17 ], supramolecular chemistry [ 18 , 19 , 20 , 21 ], and the other materials science especially for nanostructured materials [ 22 , 23 , 24 , 25 ]. Various fabrication techniques including the self-assembled monolayer (SAM) method [ 26 , 27 , 28 ], Langmuir–Blodgett (LB) technique [ 29 , 30 ], and layer-by-layer (LbL) assembly [ 31 , 32 ] have indispensable roles in the developments of functional materials as well as contributions with emerging materials such as nanocarbon materials [ 33 , 34 ] and nanoporous materials [ 35 , 36 ].…”