Carbon dots based fluorescent sensor for sensitive determination of hydroquinone

Bao

ACS Appl. Polym. Mater.

et al. 2019

β-Glucosidase is associated with many diseases, so it is important to be able to accurately determine β-glucosidase activity. Here, we describe a turn-on fluorescence method for sensitively determining β-glucosidase activity. The method involves the formation of water-soluble fluorescent polymer nanoparticles. β-Glucosidase initially hydrolyzes the β-arbutin substrate to release hydroquinone (1,4-dihydroxybenzene). The hydroquinone then forms cross-links between branched polyethylenimine molecules to give fluorescent water-soluble polymer nanoparticles. The polymer nanoparticles were found to fluoresce with excitation and emission peaks at 373 and 510 nm, respectively, and the fluorescence intensity was related to the β-glucosidase activity. The fluorescence intensity could therefore be used to determine the β-glucosidase activity. The lowest β-glucosidase activity that could be determined was 0.4 U L–1, and the dynamic linear range was 1.0–36.0 U L–1. The method was very selective for β-glucosidase activity and was not sensitive to other proteins. The method was successfully used to determine β-glucosidase in spiked samples containing human serum albumin at a concentration of 0.5%, and the recoveries were 95.2%–104.5%.

Section: Resultsmentioning

confidence: 99%

“Turn-On” Fluorescence Determination of β-Glucosidase Activity Using Fluorescent Polymer Nanoparticles Formed from Polyethylenimine Cross-Linked with Hydroquinone

Bao

ACS Appl. Polym. Mater.

et al. 2019

“…They are extensively used in rubber, medicine, pesticide, cosmetics, paint, and photography. , However, recent studies have indicated that CC and HQ are potential carcinogens to human. − Since CC and HQ are included in the list of environmental pollutants, , the fabrication of a convenient and fast means for selective detection of CC and HQ is necessary. Nowadays, different analytical methods have greatly contributed to determining CC and HQ, including high-performance liquid chromatography (HPLC), , electrochemistry, , chemiluminescence, and fluorescence. , Nevertheless, the HPLC method requires time-consuming procedures, cumbersome sample preparation, and expensive equipment. On the other hand, electrochemical methods can be used to selectively determine CC and HQ, but they have relatively low stability in field analysis, and the electrodes should be modified.…”

Section: Introductionmentioning

confidence: 99%

Electrodeposition-Assisted Rapid Preparation of Pt Nanocluster/3D Graphene Hybrid Nanozymes with Outstanding Multiple Oxidase-Like Activity for Distinguishing Colorimetric Determination of Dihydroxybenzene Isomers

Qiu

ACS Appl. Mater. Interfaces

2020

Here, we demonstrate a facile bottom-up strategy to fabricate Pt nanoclusters (Pt NCs) grafted onto three-dimensional graphene foam (3D GF) assisted by cetyltrimethyl ammonium bromide (CTAB) using the electrodeposition method. The homogeneous grafting of Pt NC onto 3D GF is due to the formation of hemimicelles above some CTAB concentration. With the unique nanocluster structure and the high content of Pt0, the Pt NC/3D GF nanohybrid exhibits extremely high activity and shows higher reusability and stability. Apart from the intrinsic oxidase-like activity with 3,3′,5,5′-tetramethylbenzidine (TMB) as the substrate, the Pt NC/3D GF nanohybrid can act simultaneously as an effective polyphenol oxidase (PPO) mimic, such as tyrosinase, catechol oxidase, and laccase. More importantly, utilizing intrinsic catechol oxidase-like activity and the oxidase-like activity with TMB as the substrate of the nanohybrid, distinguishing colorimetric determination of dihydroxybenzene isomers (catechol and hydroquinone) is performed. Distinguishing colorimetric analysis of dihydroxybenzene isomers was first developed using nanozymes. The present work provides a simple bottom-up approach for the reasonable fabrication of various nanostructured nanozymes with excellent performance using the electrodeposition method assisted with surfactants.

“…Furthermore, selectivity testing of the CD sensor using other phenol substances, such as dopamine and catechol, and other metal cations, including Fe 3+ , has proven the high specificity of the sensor toward hydroquinone. A low LOD of 0.1 μM and linear detection range of 0.1–50 μM were achieved [ 250 ]. Bisphenol A is known to have deleterious health effects even at sub-ng dosage and may lead to illnesses such as impaired reproductive capacity and diabetes; hence, the need for rapid, accurate, and reliable bisphenol detection is of utmost importance.…”

Section: Sensing Applicationsmentioning

confidence: 99%

Biogreen Synthesis of Carbon Dots for Biotechnology and Nanomedicine Applications

2018

Over the past decade, carbon dots have ignited a burst of interest in many different fields, including nanomedicine, solar energy, optoelectronics, energy storage, and sensing applications, owing to their excellent photoluminescence properties and the easiness to modify their optical properties through doping and functionalization. In this review, the synthesis, structural and optical properties, as well as photoluminescence mechanisms of carbon dots are first reviewed and summarized. Then, we describe a series of designs for carbon dot-based sensors and the different sensing mechanisms associated with them. Thereafter, we elaborate on recent research advances on carbon dot-based sensors for the selective and sensitive detection of a wide range of analytes, including heavy metals, cations, anions, biomolecules, biomarkers, nitroaromatic explosives, pollutants, vitamins, and drugs. Lastly, we provide a concluding perspective on the overall status, challenges, and future directions for the use of carbon dots in real-life sensing.