Carbon dioxide transfer in biochemical reactors

Ho, Chester S.; Smith, Mark D.; Shanahan, John F.

doi:10.1007/bfb0004427

Cited by 33 publications

(25 citation statements)

References 43 publications

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“…This is primarily due to the complexities associated with q CO2 estimation and the method presented in this study (Eqs. [2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20] 55 while values in the 0.67-0.73 range were seen for hybridoma cells in a 100-mL bioreactor operated in continuous mode. 56 For batch culture of hybridoma cells, changes in DO concentrations substantially impacted both q O2 and q CO2 but the RQ was unchanged with values of 1.30, 1.31, and 1.33, respectively, at 60, 1, and 0% DO (these correspond to 35 and 62.5% q O2 reductions at 1 and 0% DO, respectively, compared to that at 60% DO).…”

Section: Rq Estimation In Mammalian Cell Culturesmentioning

confidence: 95%

“…The equilibria of these dissociation reactions are strong functions of pH, temperature, and ionic strength that must be accounted for during q CO2 estimation. While there are several reports on q CO2 estimation in microbial systems, [19][20][21][22][23][24] there are only a few on mammalian cell chemostat and batch studies 1,25,26 and none for perfusion systems. One unique attribute of the perfusion system is cell density control by cell bleed and contributions from this stream must be accounted for in q CO2 mass balance expressions.…”

Section: Introductionmentioning

confidence: 97%

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Estimating cell specific oxygen uptake and carbon dioxide production rates for mammalian cells in perfusion culture

Goudar

Piret

Konstantinov

2011

Biotechnology Progress

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We present robust methods for online estimation of cell specific oxygen uptake and carbon dioxide production rates (q(O2) and q(CO2), respectively) during perfusion cultivation of mammalian cells. Perfusion system gas and liquid phase mass balance expressions for oxygen and carbon dioxide were used to estimate q(O2), q(CO2) and the respiratory quotient (RQ) for Chinese hamster ovary (CHO) cells in perfusion culture over 12 steady states with varying dissolved oxygen (DO), pH, and temperature set points. Under standard conditions (DO = 50%, pH = 6.8, T = 36.5°C), q(O2) and q(CO2) ranges were 5.14-5.77 and 5.31-6.36 pmol/cell day, respectively, resulting in RQ values of 0.98-1.14. Changes to DO had a slight reducing effect on respiration rates with q(O2) and q(CO2) values of 4.64 and 5.47, respectively, at DO = 20% and 4.57 and 5.12 at DO = 100%. Respiration rates were lower at low pH with q(O2) and q(CO2) values of 4.07 and 4.15 pmol/cell day at pH = 6.6 and 4.98 and 5.36 pmol/cell day at pH = 7. Temperature also impacted respiration rates with respective q(O2) and q(CO2) values of 3.97 and 4.02 pmol/cell day at 30.5°C and 5.53 and 6.25 pmol/cell day at 37.5°C. Despite these changes in q(O2) and q(CO2) values, the RQ values in this study ranged from 0.98 to 1.23 suggesting that RQ was close to unity. Real-time q(O2) and q(CO2) estimates obtained using the approach presented in this study provide additional quantitative information on cell physiology both during bioprocess development and commercial biotherapeutic manufacturing.

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Section: Rq Estimation In Mammalian Cell Culturesmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 97%

Estimating cell specific oxygen uptake and carbon dioxide production rates for mammalian cells in perfusion culture

Goudar

Piret

Konstantinov

2011

Biotechnology Progress

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“…137 The growth inhibition of microorganisms in MA is determined by the concentration of dissolved CO 2 in the product. 138,139 The preservation effect of MAP is due to the drop in surface pH in MA products because of the acidic effect of dissolved CO 2 , but this could not entirely explain all of CO 2 's bacteriostatic effect. 140 The possibility of intracellular accumulation of CO 2 would upset the normal physiological equilibrium by slowing down enzymatic processes.…”

Section: 130mentioning

confidence: 99%

Packaging Interventions in Low Temperature Preservation of Fish- A Review

2016

MOJFPT

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“…The effect of ice and a controlled atmosphere on muscle may be due to greater CO 2 solubility in the muscle; this would reduce the water holding capacity [17,20], presumably thanks to a reduction of the pH. According to Henry's law [21], CO 2 solubility will increase when the temperature falls [22] and the amount of water in the muscle increases, both of which will occur with the melting of the ice. The flavour loss is probably due to the leaching out of components such as free amino acids and organic phosphates [23].…”

Section: Effects Of the Atmosphere On Shrimps With Additivesmentioning

confidence: 99%

Controlled atmosphere as coadjuvant to chilled storage for prevention of melanosis in shrimps (Parapenaeus longirostris)

Martínez-Álvarez

Montero

Gómez‐Guillén³

2004

Eur Food Res Technol

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The effect of a controlled atmosphere with high CO 2 and low O 2 levels on melanosis in deepwater pink shrimps (Parapenaeus longirostris) treated with sulphites was studied in samples in chilled storage. The application of atmosphere in shrimps without antimelanotics did not inhibit melanosis. Shrimps treated with 4% sulphites in combination with a concentration of 53% CO 2 and 7% O 2 totally inhibited darkening during storage. Covering with crushed ice produced an undesirable shrinking of muscle shrimp after cooking. The application of lower concentrations of CO 2 (45%) reduced the effectiveness in preventing darkening, compared with 53% CO 2 , except where the melanosis inhibitor was 4-hexylresorcinol (0.1%). The residual effect of the gas when the shrimps were kept in a controlled atmosphere for 24 h was insufficient to prevent melanosis during further cold storage.

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Carbon dioxide transfer in biochemical reactors

Cited by 33 publications

References 43 publications

Estimating cell specific oxygen uptake and carbon dioxide production rates for mammalian cells in perfusion culture

Estimating cell specific oxygen uptake and carbon dioxide production rates for mammalian cells in perfusion culture

Packaging Interventions in Low Temperature Preservation of Fish- A Review

Controlled atmosphere as coadjuvant to chilled storage for prevention of melanosis in shrimps (Parapenaeus longirostris)

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