Carbon Dioxide (CO2)-Laser Therapy Cures Macroscopic Lesions, but Viral Genome Is Not Eradicated in Men With Therapy-Resistant HPV Infection

Absence ofp53 mutations in benign and pre-malignant male genital lesions with over-expressed p53 protein

“…To demonstrate DNA specific for HPV types 6, 11, 16, 18, 31, 33 and 35, in situ hybridization with both digoxigenin-and biotinlabeled HPV DNA probes and enzymatic detection were used to study paraffin-embedded or frozen sections, as described previously in detail (Lassus et al, 1994). Briefly, mixtures of the Int.…”

“…Biopsies were obtained for diagnostic purposes from 13 male patients followed up at the venereal outpatient clinic of the Department of Dermatology and Venereal Diseases, Helsinki University Hospital. The histopathological diagnosis of the biopsies was made by an experienced pathologist (Lassus et al, 1994). Patients had suffered from genital warts for 0.5-5 years (range) before the first biopsy was obtained.…”

“…Follow-up biopsies were obtained from therapy-resistant lesions that had not responded to local podophyllotoxin or cryotherapy during the first 3 months of follow-up. Thereafter, lesions were treated with CO 2 -laser evaporation (Lassus et al, 1994). Most biopsies were routinely fixed in 4% neutral formalin and embedded in paraffin.…”

“…Amplification of HPV 16/E6-specific DNA and subsequent Southern blotting was performed on biopsies negative for HPV 16/18 by in situ hybridization (Lassus et al, 1994). Each amplification included a positive and several negative control samples, and parallel amplification for ␤-globin was performed (Lassus et al, 1994). …”

Thermotherapy in dermatologic infections

“…CO 2 laser ablation, which creates thermal damage via absorption of the laser's 10,600-nm wavelength by water in tissue, has been used to treat condyloma acuminatum and recalcitrant verrucae, with more recent experience in the treatment of leishmaniasis. [48][49][50][51][52][53][54][55][56] There is also reported experience with neodymium-doped yttrium aluminium garnet (Nd:YAG) laser hyperthermia used in the treatment of verrucae. 57,58 The development of localized current field radiofrequency has been an advance in the field of thermotherapy and has been used in the treatment of acne and mycobacterial infections but more often in the treatment of leishmaniasis.…”