Carbon-Based Nanobiohybrid Thin Film for Amperometric Glucose Sensing

Abstract

This pioneering study involved the fabrication of a new class of nanohybrid-based electrochemical glucose biosensor. First, three-dimensional (3D) graphene was fabricated as a platform of multiwalled carbon nanotube (MWCNT). Then, it was used to immobilize glucose oxidase (GOD) on nanohybrid thin film via the entrapment technique. The modified glucose biosensor indicated excellent biocatalytic activity toward the glucose measurment with a sensitivity of up to 49.58 μA mM–1 cm–2 and a wide linear dynamic range up to 16 mM. The fabricated biosensor shows an excellent stability of 87.8%, with its current diminishing after 3 months. This facile and simple electrochemical method for glucose monitoring using a modified glassy carbon electrode (GCE) by 3DG-MWCNT-GOD could open new avenues in producing of a inexpensive and sensitive glucose nanobiosensors.

Direct-Electron-Transfer Bio-Nanoink with Single-Walled Carbon Nanotube and Aspergillus terreus var. aureus Flavin Adenine Dinucleotide Glucose Dehydrogenase

“…These results suggest that the bio-nanoink prevents leaching and contamination of Ata GDH and SWCNT components. Table shows a comparison of the characteristics and performances of the biosensor fabricated by bio-nanoink are compared with those for other glucose biosensors. − It is confirmed that the present sensor with the DET bio-nanoink exhibited better performance compared with most of the other enzyme glucose biosensors. Although there may be some better and simpler sensors in literature than the proposed one, these use glucose oxidase, which is a disadvantage because enzyme-catalytic reaction depends strongly on the concentration of dissolved oxygen at higher glucose concentration.…”

“…This characteristic is often observed at the other biosensors. [2][3][4]8,9 It does not affect the accuracy of quantification. The effects of the interfering compounds Lascorbic acid (AA) and uric acid (UA) on the sensing characteristics are negligible (bottom inset of Figure 7A), suggesting that the biosensor fabricated by bio-nanoink can be used with physiological samples.…”

“…Glucose biosensors with biospecific enzyme reactions have become an active research area because they help diabetes mellitus patients to monitor their daily sugar level . A flavin adenine dinucleotide-dependent glucose oxidase (FAD-GOx, EC 1.1.3.4) Aspergillus niger have been used as the glucose-specific enzyme for the biosensors. − There are two major drawbacks in the usage of FAD-GOx: (i) Its enzyme-catalytic reaction depends strongly on the concentration of dissolved oxygen. For example, data obtained at highlands where the oxygen concentration is relatively thin is often inaccurate.…”

Enhanced Electrochemical Activity of a Hollow Carbon Sphere/Polyaniline-Based Electrochemical Biosensor for HBV DNA Marker Detection

“…The diversity of carbonic structures does not end at the fullerene, carbon nanotubes, and graphene. , Nowadays, the fabrication of hollow and porous carbon nanostructures, produced via carbonization of organic materials, is extensively progressing. Well-defined pore structures, remarkably high surface areas, extremely short mass diffusion and transport resistance, and precisely tuned pore sizes are some highlighted features of hollow carbon structures .…”

Achieving a Stable High Surface Excess of Glucose Oxidase on Pristine Multiwalled Carbon Nanotubes for Glucose Quantification

“…Recently, graphene-based materials have garnered attention for sensing applications due to their biocompatibility and conducting nature. Chen et al, reported few CNT-graphene based composite materials, such as GO-MWCNT hybrid, electrochemically reduced rGO-MWCNT, and Au nanoparticle-decorated graphene-CNT hybrid. − Other graphene-based composites that have been reported in the literature for GOx immobilization include a graphene-CNT hybrid film grown by 3D seamless chemical vapor deposition, PDDA-capped Au nanoparticles incorporated on functionalized graphene-MWCNT nanocomposite, a 3D graphene layer as a platform for GOx-MWCNT composite immobilization, and rGO-CNTs-carbon fiber felt-based electrode . Although these CNTs based electrode materials have shown reasonable sensitivity, they suffer from some several drawbacks such as cumbersome methodology to fabricate the electrode film and requirement of expensive chemicals, surfactants, ionic liquids, and polymers as well as Pd, Pt, Au, and Ag metal nanoparticles that require extensive procedures made to activate modified electrode.…”