Carbon and iron ion radiation-induced cytotoxicity and transformation in vitr

Zhou, Zhaozong; Ware, Jeffrey H.; Kennedy, Ann R.

doi:10.3892/ol.2011.342

Cited by 3 publications

(1 citation statement)

References 11 publications

(17 reference statements)

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“…Direct or indirect outcomes of radiation-induced damage on biomolecules as well as cellular structures in the form of mutations, DNA damage, cell death, phenotypic changes and increased oxidative stress levels (21) can exacerbate the risk of degenerative diseases (e.g., cardiovascular and circulatory) that are usually associated with aging (3). While the intracellular effects of HZE radiation, such as DNA damage and repair (65, 66), persistent oxidative stress (67) and cell cytotoxicity (68), have been studied, comprehensive work to elucidate how HZE particles affect heart tissue and cardiac function is still in its infancy. Previously published studies have highlighted the acute effects of high-dose radiation in cardiomyocytes (69, 70) with the primary focus on upregulation of gene transcription (71).…”

Section: Discussionmentioning

confidence: 99%

Different Sequences of Fractionated Low-Dose Proton and Single Iron-Radiation-Induced Divergent Biological Responses in the Heart

et al. 2017

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Deep-space travel presents risks of exposure to ionizing radiation composed of a spectrum of low-fluence protons (H) and high-charge and energy (HZE) iron nuclei (e.g., Fe). When exposed to galactic cosmic rays, each cell in the body may be traversed byH every 3-4 days and HZE nuclei every 3-4 months. The effects of low-dose sequential fractionated H or HZE on the heart are unknown. In this animal model of simulated ionizing radiation, middle-aged (8-9 months old) male C57BL/6NT mice were exposed to radiation as follows: group 1, nonirradiated controls; group 2, three fractionated doses of 17 cGyH every other day (H × 3); group 3, three fractionated doses of 17 cGy H every other day followed by a single low dose of 15 cGyFe two days after the final H dose (H × 3 + Fe); and group 4, a single low dose of 15 cGyFe followed (after 2 days) by three fractionated doses of 17 cGy H every other day (Fe + H × 3). A subgroup of mice from each group underwent myocardial infarction (MI) surgery at 28 days postirradiation. Cardiac structure and function were assessed in all animals at days 7, 14 and 28 after MI surgery was performed. Compared to the control animals, the treatments that groups 2 and 3 received did not induce negative effects on cardiac function or structure. However, compared to all other groups, the animals in group 4, showed depressed left ventricular (LV) functions at 1 month with concomitant enhancement in cardiac fibrosis and induction of cardiac hypertrophy signaling at 3 months. In the irradiated and MI surgery groups compared to the control group, the treatments received by groups 2 and 4 did not induce negative effects at 1 month postirradiation and MI surgery. However, in group 3 after MI surgery, there was a 24% increase in mortality, significant decreases in LV function and a 35% increase in post-infarction size. These changes were associated with significant decreases in the angiogenic and cell survival signaling pathways. These data suggest that fractionated doses of radiation induces cellular and molecular changes that result in depressed heart functions both under basal conditions and particularly after myocardial infarction.

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Section: Discussionmentioning

confidence: 99%

Different Sequences of Fractionated Low-Dose Proton and Single Iron-Radiation-Induced Divergent Biological Responses in the Heart

et al. 2017

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Gene expression profiling in undifferentiated thyroid carcinoma induced by high-dose radiation

Bang¹,

Choi²,

Kim³

et al. 2016

Journal of Radiation Research

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Published gene expression studies for radiation-induced thyroid carcinogenesis have used various methodologies. In this study, we identified differential gene expression in a human thyroid epithelial cell line after exposure to high-dose γ-radiation. HTori-3 cells were exposed to 5 or 10 Gy of ionizing radiation using two dose rates (high-dose rate: 4.68 Gy/min, and low-dose rate: 40 mGy/h) and then implanted into the backs of BALB/c nude mice after 4 (10 Gy) or 5 weeks (5 Gy). Decreases in cell viability, increases in giant cell frequency, anchorage-independent growth in vitro, and tumorigenicity in vivo were observed. Particularly, the cells irradiated with 5 Gy at the high-dose rate or 10 Gy at the low-dose rate demonstrated more prominent tumorigenicity. Gene expression profiling was analyzed via microarray. Numerous genes that were significantly altered by a fold-change of >50% following irradiation were identified in each group. Gene expression analysis identified six commonly misregulated genes, including CRYAB, IL-18, ZNF845, CYP24A1, OR4N4 and VN1R4, at all doses. These genes involve apoptosis, the immune response, regulation of transcription, and receptor signaling pathways. Overall, the altered genes in high-dose rate (HDR) 5 Gy and low-dose rate (LDR) 10 Gy were more than those of LDR 5 Gy and HDR 10 Gy. Thus, we investigated genes associated with aggressive tumor development using the two dosage treatments. In this study, the identified gene expression profiles reflect the molecular response following high doses of external radiation exposure and may provide helpful information about radiation-induced thyroid tumors in the high-dose range.

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