Carbon-13 CP/MAS NMR and DR-FTIR spectroscopic studies of sugarcane distillery waste

Abstract

Benke, M. B., Mermut, A. R. and Chatson, B. 1998. Carbon-13 CP/MAS NMR and DR-FTIR spectroscopic studies of sugarcane distillery waste. Can. J. Soil Sci. 78: 227-236. The application of a sugarcane distillery waste known as vinasse to agricultural land has become a common practice in Brazil. The vinasse samples used in this study were collected from several sugarcane distilleries in Northeastern Brazil. These samples were fractionated into dissolved organic carbon (DOC) and particulate organic carbon (POC) fractions. Unfractionated and fractionated vinasse were studied using 13 C cross-polarization and magicangle spinning nuclear magnetic resonance (CP/MAS NMR) spectroscopy as well as diffuse reflectance Fourier-transform infrared (DR-FTIR) spectroscopy. Approximately 79 to 92% of the total unfractionated vinasse dry matter was in the form of DOC fraction. O-alkyl C (42-53% of the total C) and carboxyl C (12-25% of the total C) comprised a significant portion of the 13 C NMR spectra of the DOC fraction. The presence of carbohydrates and COOH/COO -was suggested by the DR-FTIR as well. Both 13 C NMR and DR-FTIR spectra of this fraction were generally similar to the spectra of the fulvic acid (FA) fraction of soil and sewage sludge. The spectra of DOC differed from the FA fraction in that they showed smaller amounts of aromatic C and had an absence of amide group (bands at 1650 cm -1 and 1540 cm -1 ). In the POC fraction, O-alkyl (17-52% of the total C) and alkyl C (15-41% of the total C) were the major contributors. The peaks at 62 ppm, 72 ppm, 84 ppm and 105 ppm in the O-alkyl region indicate the presence of cellulose and/or hemicellulose. The alkyl group was comprised mainly of long-chain structures. The total N content in this fraction is ~3-7 times as much as in the DOC fraction. The presence of amino acids in the POC fraction was suggested by both 13 C NMR and DR-FTIR spectra.

Anaerobic digestion of vinasse from sugarcane ethanol production in Brazil: Challenges and perspectives

“…In the annexed plants, which produce both sugar and ethanol, vinasse derives from both molasses and juice. Regardless of its origin, the main organic compounds present in sugarcane vinasse reported in the literature consist of organic acids (mainly lactate and acetate), as well as alcohols (mainly glycerol and ethanol) and a minor amount of carbohydrates [14,[54][55][56][57]. Table 3 compiles studies in which vinasse characterization was performed, presenting the main parameters that define its composition according to the fermentation feedstock [47,48,[58][59][60][61][62].…”

Pyrolysis/gas chromatography/mass spectrometry monitoring of fungal‐biotreated distillery wastewater using Trametes sp. I‐62 (CECT 20197)

“…Yeast cells and sugar-cane residues have been evidenced in some fractions of sugar-cane distillery waste, by the spectroscopic signals of cellulose, lipids and amino acids detected using NMR and FTIR. 38 In general, humic acids and melanoidins share a series of common chemical features: they are similar in elemental composition, spectroscopic characteristics and environmental stability. 10 In addition, these macromolecular substances are random in structure and highly polydisperse in molecular weight.…”

Vinasse from Sugarcane Ethanol Production: Better Treatment or Better Utilization?

“…Vinasse is also characterized as a feedstock rich in phenolic compounds and melanoidins (FitzGibbon et al, 1998). Using NMR and FTIR, Benke et al (1998) detected levels of cellulose and hemicellulose in vinasse, which are derived from the grinding conditions of sugarcane. Recent development on lignocellulosic ethanol production from sugarcane bagasse may further increase the fiber content of vinasse, as that the various methods applied to sugarcane bagasse pretreatment will likely affect the production of liquid byproducts (Moraes et al, 2015).…”