Carbohydrate Sources, Alanine and Calcium for in Vitro Multiplication of Eucalyptus Cloeziana F. Muell.

Silva, André Luís Lopes da; Tormen, Gabriela Cristina Rech; Figueiredo, Alexssandra Jéssica Rondon de; Ribeiro, Anatálya dos Santos; Santos, Leandro Freire dos; Araújo, Jairton Fraga; Brondani, Gilvano Ebling

doi:10.21826/2446-8231201873309

Cited by 4 publications

(2 citation statements)

References 25 publications

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“…However, despite its potential for wood production, E. microcorys has been less employed in forest composition than other eucalypts species, and few scienti c reports are found on the topic [3]. One of the main barriers for propagation is the low regenerative capacity caused by the ontogenetic aging of tissues [4], which reduces regeneration capacity and hinders adventitious rooting [5].…”

Section: Introductionmentioning

confidence: 99%

WITHDRAWN: Reduction in Microbial Manifestation on In Vitro Culture of Eucalyptus Microcorys

Atala

Faria

Molinari

et al. 2021

Preprint

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Micropropagation is one of the main applications of tissue culture of plants. It allows rejuvenation and, consequently, improves explant rooting of woody species. However, one of its main problems is the risk of microbial manifestation. Here, we aimed to evaluate the survival and the reduction in microbial manifestations on in vitro cultures of Eucalyptus microcorys with the addition of chemical agents (sodium hypochlorite or streptomycin) to WPM culture medium. We used in vitro cultivated explants from adventitious shoots from two E. microcorys mother plants, over at 44 years-old. We drew from the multiplication phase explants with clumps of 8 to 12 shoots that displayed bacterial manifestation and submitted them to microbe control, adding different chemical agents at different concentrations to the culture medium: sodium hypochlorite (NaClO) and the antibiotic streptomycin. The results indicated that the best concentrations to reduce the manifestation of microorganisms on in vitro cultivation were 100 mg L− 1 of streptomycin and sodium hypochlorite with 0.003% of active chlorine, since these concentrations maintained 100% of the explants alive and reduced microbial manifestation more efficiently.

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Section: Introductionmentioning

confidence: 99%

WITHDRAWN: Reduction in Microbial Manifestation on In Vitro Culture of Eucalyptus Microcorys

Atala

Faria

Molinari

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

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“…Eucalyptus is the most cultivated forest genus in Brazil, but, despite its interesting characteristics, E. cloeziana is little used. The reason for this is related to reproductive difficulties, as the traditional form of propagation of Eucalyptus by cloning is not viable due to the low rooting capacity of cuttings (Tormen et al, 2018), while its seeds have a lower germination rate than other species of the same genus despite being produced in large quantities. In recognition of these phenomena, several authors (Clarke et al, 2009;Erickson et al, 2016) have suggested that this low germination characteristic may not be related to possible low seed quality, but rather to the high proportion of indistinguishable seed impurities within the seed lots post-harvest.…”

Section: Introductionmentioning

confidence: 99%

Processing Eucalyptus cloeziana seed lots to improve purity, germination and vigor

Michelon

Vieira

Panobianco

2021

Sci. agric. (Piracicaba, Braz.)

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Eucalyptus cloeziana is one of the highest-density wood species offering significant added value, frequently used in the construction and furniture industries. Despite its suitability, it may be overlooked on account of its propagation difficulties due either to its low rooting cutting capacity or to its seeds having a low germination rate perhaps attributable to the high proportion of indistinguishable impurities within the seed lots. This study aimed to develop an efficient technique for Eucalyptus cloeziana seed processing to increase the physical purity and, consequently, the physiological quality of the seed lot. Three seed lots, separated by size in sieves with 1.18, 1.00, and 0.84 mm square openings, were used. X-ray analyses were carried out, the number of normal seedlings and germination rate identified and the percentage and speed of seedling emergence under greenhouse conditions ascertained. The greenhouse evaluation showed that the seed retained in the 1.18 mm sieve presented viability and vigor twice that of the control. Therefore, the processing technique by size classification is efficient in terms of improving the physical and physiological performance of Eucalyptus cloeziana seed lots.

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Effect of light intensity on in vitro introduction and multiplication of Eucalyptus grandis × Eucalyptus urophylla

Souza

Fernandes

Silva

et al. 2021

In Vitro Cell.Dev.Biol.-Plant

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Carbohydrate Sources, Alanine and Calcium for in Vitro Multiplication of Eucalyptus Cloeziana F. Muell.

Cited by 4 publications

References 25 publications

WITHDRAWN: Reduction in Microbial Manifestation on In Vitro Culture of Eucalyptus Microcorys

WITHDRAWN: Reduction in Microbial Manifestation on In Vitro Culture of Eucalyptus Microcorys

Processing Eucalyptus cloeziana seed lots to improve purity, germination and vigor

Effect of light intensity on in vitro introduction and multiplication of Eucalyptus grandis × Eucalyptus urophylla

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