Carbohydrate patterns, cellular lipoquinones, fatty acids and phospholipids of the genus Pasteurella sensu stricto

Engelhard, E.; Kroppenstedt, Reiner M.; Mutters, Reinier; Mannheim, W.

doi:10.1007/bf00193849

Cited by 11 publications

(8 citation statements)

References 30 publications

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“…Negative reactions are also observed for the H 2 S/TSI test, tolerance to KCN, methyl red, Voges-Proskauer, gas from nitrate, arginine dehydrolase, lysine decarboxylase, phenylalanine deaminase, gelatinase, growth on Tween 20 and 80, gas from ( (Table 1). Previous investigations of the type strain (MCCM 00102 T ) have shown Q-8, MK-8 and DMK-8 as quinones (Engelhard et al, 1991;Kainz et al, 2000) ( Table S3). Polyamines in the type strain were reported as Fig.…”

Section: Characteristicmentioning

confidence: 80%

“…In the type strain, the major fatty acids were reported as C 16 : 0 , C 16 : 1 v7c and C 14 : 0 , with minor amounts of C 18 : 0 , C 18 : 1 v9c and traces of C 20 : 4 cis5, C 14 : 0 3-OH, C 20 : 1 trans11, C 20 : 0 , C 16 : 1 C, C 15 : 0 , C 14 : 5 and C 12 : 0 (Engelhard et al, 1991).…”

Section: Characteristicmentioning

confidence: 97%

“…All three types of molecules (Q-8, DMK-8, MK-8) have also been found in the other members of Pasteurella sensu stricto investigated including the type strain of P. multocida, although in different proportions depending on the investigation (Table S3). The reason for different proportions of ubiquinones, menaquinones and demethylmenaquinones seems to be the higher degree of aeration used during cultivation by Kainz et al (2000) than in the investigations of Kroppenstedt & Mannheim (1989) and Engelhard et al (1991). rpoB gene sequence-based classification has previously been used with members of the Pasteurellaceae (Angen et al, 2003;Korczak et al, 2004).…”

Section: Analysis Of Fatty Acids (Strains P683mentioning

confidence: 99%

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Classification of Pasteurella species B as Pasteurella oralis sp. nov.

Bertelsen

Bojesen

Bisgaard

2012

International Journal of Systematic and Evolutionary Microbiology

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Pasteurella species B has so far only been reported from the oral cavity of dogs, cats and a ferret. In the present study, information from 15 recent isolates from different sources, including African hedgehogs (Atelerix albiventris), banded mongoose (Mungos mungo), Moholi bushbabies (Galago moholi) and pneumonia of a cat, were compared to five strains investigated previously from bite wounds in humans inflicted by a cat and dog and from gingiva of a cat. rpoB gene sequence comparison showed that 17 isolates, including the reference strain (CCUG 19794 T ), had identical sequences, whereas two were closely related and demonstrated 97.9 and 99.6 % similarity to strain CCUG 19794 T , respectively; the type strain of Pasteurella stomatis was the most closely related strain, with 92.3 % similarity. This is within the mean range (76-100 %) of rpoB gene sequence similarity between species of the same genus within the family Pasteurellaceae. 16S rRNA gene sequencing of four strains selected based on rpoB sequence comparison showed at least 99.7 % similarity between strains of Pasteurella species B, with 96.2 % similarity to the type strain of the closest related species (Pasteurella canis), indicating that Pasteurella species B should have separate species status. Separate species status was also documented when recN sequence comparisons were converted to a genome similarity of 93.7 % within Pasteurella species B and 59.0 % to the type strain of the closest related species (P. canis). Based on analysis of the phylogenetic and phenotypic data, and since most isolates originate from the oral cavities of a diverse group of animals, it is suggested that these bacteria be classified as Pasteurella oralis sp. nov.; the type strain is P683 T (5CCUG 19794 T 5CCM 7950T 5strain 23193 T 5MCCM 00102 T ), obtained from a cat. Previous reports of the type strain have shown ubiquinone-8, demethylmenaquinone-8 and menaquinone-8 as the major quinones. Polyamines in the type strain were reported as diaminopropane, putrescine, cadaverine, symnorspermidine, spermidine and spermine in a previous investigation, and the major fatty acids of the type strain were reported to be C 16 : 0 , C 16 : 1 v7c and C 14 : 0 , with minor amounts of C 18 : 0 and C 18 : 1 v9c. The DNA G+C content of the type strain has been reported to be 40.0 mol%.

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Section: Characteristicmentioning

confidence: 80%

Section: Characteristicmentioning

confidence: 97%

Section: Analysis Of Fatty Acids (Strains P683mentioning

confidence: 99%

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Classification of Pasteurella species B as Pasteurella oralis sp. nov.

Bertelsen

Bojesen

Bisgaard

2012

International Journal of Systematic and Evolutionary Microbiology

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“…MK7 has been reported in low amounts in some species of the genus Avibacterium (Kroppenstedt & Mannheim, 1989;Kainz et al, 2000) and in moderate amounts in members of the genera Actinobacillus, Mannheimia and Bibersteinia (Kroppenstedt & Mannheim, 1989). MK8 and ubiquinone with chain length of 8 were found by Engelhard et al (1991) in the genus Gallibacterium and this separates the genus from the taxa 23 and 24 of Bisgaard (Table S2).…”

Section: Horsevaenget 40 Dk-4130 Viby Sjaelland Denmarkmentioning

confidence: 95%

Investigation of taxa of the family Pasteurellaceae isolated from Syrian and European hamsters and proposal of Mesocricetibacter intestinalis gen. nov., sp. nov. and Cricetibacter osteomyelitidis gen. nov., sp. nov.

Nicklas

Bisgaard²

2014

International Journal of Systematic and Evolutionary Microbiology

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Eleven strains from hamster of Bisgaard taxa 23 and 24, also referred to as Krause’s groups 2 and 1, respectively, were investigated by a polyphasic approach including data published previously. Strains showed small, regular and circular colonies with smooth and shiny appearance, typical of members of the family Pasteurellaceae . The strains formed two monophyletic groups based on 16S rRNA gene sequence comparison to other members of the family Pasteurellaceae . Partial rpoB sequencing as well as published data on DNA–DNA hybridization showed high genotypic relationships within both groups. Menaquinone 7 (MK7) was found in strains of both groups as well as an unknown ubiquinone with shorter chain length than previously reported for any other member of the family Pasteurellaceae . A new genus with one species, Mesocricetibacter intestinalis gen. nov., sp. nov., is proposed to accommodate members of taxon 24 of Bisgaard whereas members of taxon 23 of Bisgaard are proposed to represent Cricetibacter osteomyelitidis gen. nov., sp. nov. Major fatty acids of type strains of type species of both genera are C14 : 0, C14 : 0 3-OH/iso-C16 : 1 I, C16 : 1ω7c and C16 : 0. The two genera are clearly separated by phenotype from each other and from existing genera of the family Pasteurellaceae . The type strain of Mesocricetibacter intestinalis is HIM 933/7T ( = Kunstyr 246/85T = CCUG 28030T = DSM 28403T) while the type strain of Cricetibacter osteomyelitidis is HIM943/7T ( = Kunstyr 507/85T = CCUG 36451T = DSM 28404T).

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“…The recN sequences were further used for prediction of genome similarity values between the investigated strains and the type strains of type species of all (Kroppenstedt and Mannheim 1989;Norskov-Lauritsen et al 2005); 3, Actinobacillus (Kroppenstedt and Mannheim 1989;; 4, Pasteurella (Mutters et al 1985;Kainz et al 2000); 5, Lonepinella (Osawa et al 1995); 6, Mannheimia (Kroppenstedt and Mannheim 1989;Angen et al 1999); 7, Phocoenobacter (Foster et al 2000); 8, Gallibacterium (Engelhard et al 1991;Christensen et al 2003); 9, Histophilus (Mutters et al 1993;Angen et al 2003); 10, Volucribacter ; 11, Nicoletella (Kuhnert et al 2004); 12, Avibacterium (Kroppenstedt and Mannheim 1989;Blackall et al 2005); 13, Aggregatibacter (Kroppenstedt and Mannheim 1989;Mutters et al 1993;Norskov-Lauritsen and Kilian 2006); 14, Bibersteinia (Mutters et al 1993;Blackall et al 2007); 15, Chelonobacter (Gregersen et al 2009); 16, Basfia A. paragallinarum biovar 1 might be negative; f P. dagmatis and P. oralis positive; g Actinobacillus suis negative; h P. multocida positive; i Actinobacillus urea negative; j P. dagmatis and P. stomatis negative ? 90 % or more of the strains positive within 1-2 days; -less than 10 % of the strains positive within 14 days; d positive or negative; w weak positive; n.a.…”

Section: Phylogenetic Analysesmentioning

confidence: 99%

Frederiksenia canicola gen. nov., sp. nov. isolated from dogs and human dog-bite wounds

Korczak

Bisgaard

Kuhnert

2014

Antonie van Leeuwenhoek

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Polyphasic analysis was done on 24 strains of Bisgaard taxon 16 from five European countries and mainly isolated from dogs and human dog-bite wounds. The isolates represented a phenotypically and genetically homogenous group within the family Pasteurellaceae. Their phenotypic profile was similar to members of the genus Pasteurella. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry clearly identified taxon 16 and separated it from all other genera of Pasteurellaceae showing a characteristic peak combination. Taxon 16 can be further separated and identified by a RecN protein signature sequence detectable by a specific PCR. In all phylogenetic analyses based on 16S rRNA, rpoB, infB and recN genes, taxon 16 formed a monophyletic branch with intraspecies sequence similarity of at least 99.1, 90.8, 96.8 and 97.2 %, respectively. Taxon 16 showed closest genetic relationship with Bibersteinia trehalosi as to the 16S rRNA gene (95.9 %), the rpoB (89.8 %) and the recN (74.4 %), and with Actinobacillus lignieresii for infB (84.9 %). Predicted genome similarity values based on the recN gene sequences between taxon 16 isolates and the type strains of known genera of Pasteurellaceae were below the genus level. Major whole cell fatty acids for the strain HPA 21(T) are C14:0, C16:0, C18:0 and C16:1 ω7c/C15:0 iso 2OH. Major respiratory quinones are menaquinone-8, ubiquinone-8 and demethylmenaquinone-8. We propose to classify these organisms as a novel genus and species within the family of Pasteurellaceae named Frederiksenia canicola gen. nov., sp. nov. The type strain is HPA 21(T) (= CCUG 62410(T) = DSM 25797(T)).

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Carbohydrate patterns, cellular lipoquinones, fatty acids and phospholipids of the genus Pasteurella sensu stricto

Cited by 11 publications

References 30 publications

Classification of Pasteurella species B as Pasteurella oralis sp. nov.

Classification of Pasteurella species B as Pasteurella oralis sp. nov.

Investigation of taxa of the family Pasteurellaceae isolated from Syrian and European hamsters and proposal of Mesocricetibacter intestinalis gen. nov., sp. nov. and Cricetibacter osteomyelitidis gen. nov., sp. nov.

Frederiksenia canicola gen. nov., sp. nov. isolated from dogs and human dog-bite wounds

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