Carbohydrate microarrays for screening functional glycans

Abstract

Carbohydrate microarrays were used for the simultaneous screening of various glycans whose binding to the cell-surface lectin elicits cellular response.

Analysis of binding properties of pathogens and toxins using multivalent glycan microarrays

“…To avoid the time-consuming and difficult synthesis of multivalent glycoconjugates, we took advantage of an earlier observation showing that unmodied mono-, di-, oligo-and polysaccharides are covalently and site-specically immobilized on hydrazide-derivatized surfaces. 14,15 Accordingly, in this effort we developed a new, facile immobilization protocol for construction of mono-and multivalent carbohydrate microarrays, in which unmodied glycans are linked to mono-, di-, tri-and tetravalent hydrazide-derivatized glass slides (Fig. 1).…”

“…Finally, the acid groups on the surfaces of each slide were converted to NHS esters, which were then reacted with hydrazine to generate the target di, tri-and tetravalent hydrazide-modied slides. Carbohydrate microarrays containing mono-and multivalent glycans were prepared by printing nineteen unmodied sugars (1.0 nL, 10 and 30 mM of mono-(11-13), di- (14)(15)(16)(17) and oligosaccharides (18-29)) on mono-, di-, tri-and tetravalent hydrazide-coated glass slides by using an automatic pin-type microarrayer (Fig. 3).…”

Chemoenzymatic synthesis of heparan sulfate and heparin oligosaccharides and NMR analysis: paving the way to a diverse library for glycobiologists

“… 30 The oligosaccharide library can be coupled to the surface of a microarray for high-throughput screening of heparin-binding proteins. 31 …”

DNA Encoded Glycan Libraries as a next-generation tool for the study of glycan-protein interactions

“…[4,9,11,[47][48][49][50][51] Recognizing the importance of glycans, research has focused on synthesis and isolation of glycans from different sources, including animals and plants. [12,27,[52][53][54] Synthetic efforts have led to the availability of a large number of glycan structures for study, and the number is increasing quickly. Despite these advances, high throughput methods to investigate the functions of diverse glycan structures have lagged behind.…”