Carbohydrate components of Taenia crassiceps metacestodes display Th2-adjuvant and anti-inflammatory properties when co-injected with bystander antigen

Gómez-García, Lorena; Rivera-Montoya, Irma; Rodrı́guez-Sosa, Miriam; Terrazas, Luis I.

doi:10.1007/s00436-006-0159-2

Cited by 42 publications

(33 citation statements)

References 50 publications

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“…For example, macrophages were implicated in the resolution of dextran-sodium sulfate-induced colitis in mice infected with Schistosoma mansoni (41). Given the possibility that helminthderived molecules might induce anti-inflammatory type macrophages (7,11), we tested the effect of an extract of H. diminuta and ES products on macrophage function. Analysis of three macrophage types revealed that HdHMW and HdES significantly inhibited macrophage activation by LPS and poly(I:C), as measured by cytokine production.…”

Section: Discussionmentioning

confidence: 99%

Extracts of the Rat Tapeworm,Hymenolepis diminuta, Suppress Macrophage ActivationIn Vitroand Alleviate Chemically Induced Colitis in Mice

et al. 2010

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Analysis of parasite-host interactions can reveal the intricacies of immunity and identify ways to modulate immunopathological reactions. We assessed the ability of a phosphate-buffered saline-soluble extract of adult Hymenolepis diminuta to suppress macrophage (human THP-1 cell line, murine peritoneal macrophages) activity in vitro and the impact of treating mice with this extract on colitis induced by dinitrobenzene sulfonic acid (DNBS). A high-molecular-mass fraction of adult H. diminuta (HdHMW) or excretory/secretory products reduced macrophage activation: lipopolysaccharide (LPS)-induced interleukin-1β (IL-1β), IL-6, and tumor necrosis factor alpha (TNF-α) and poly(I:C)-induced TNF-α and IL-6 were suppressed by HdHMW. The active component in the HdHMW extract was minimally sensitive to boiling and trypsin digestion, whereas the use of sodium metaperiodate, as a general deglycosylation strategy, indicated that the immunosuppressive effect of HdHMW was at least partially dependent on a glycan: treating the HdHMW with neuraminidase and α-mannosidase failed to inhibit its blockade of LPS-induced TNF-α production by THP-1 macrophages. Mice treated with DNBS developed colitis, as typified by wasting, shortening of the colon, macroscopic and microscopic tissue damage, and an inflammatory infiltrate. Mice cotreated with HdHMW (three intraperitoneal injections) displayed significantly less inflammatory disease, and this was accompanied by reduced TNF-α production and increased IL-10 and IL-4 production by mitogen-stimulated spleen cells. However, cotreatment of mice with neutralizing anti-IL-10 antibodies had only a minor impact on the anticolitic effect of the HdHMW. We speculate that purification of the immunosuppressive factor(s) from H. diminuta has the potential to lead to the development of novel immunomodulatory drugs to treat inflammatory disease.

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Section: Discussionmentioning

confidence: 99%

Extracts of the Rat Tapeworm,Hymenolepis diminuta, Suppress Macrophage ActivationIn Vitroand Alleviate Chemically Induced Colitis in Mice

et al. 2010

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“…Interestingly, two groups of researchers have started to point out the relevance of glycoconjugates from T. crassiceps in immunomodulation. Thus, it has been demonstrated that glycans obtained from these metacestodes can modulate both innate [59] as well as acquired immune responses, and can even function as adjuvants favoring the Th2-type responses in vivo [49,50]. However, further studies are needed to define the main glycans involved in immune-modulation, as well as the target cells and the possible receptor for those molecules.…”

Section: Immunomodulation In Cysticercosismentioning

confidence: 95%

“…Specific inhibition of p38 MAPK results in substantial down-regulation of cytokine production in LPS-stimulated macrophages [47]. Thus, GC treatment during cysticercosis may influence the pro-inflammatory cytokine responses on granulomas, although a role for TLR-4 in response to Taenia antigens is still controversial [48][49][50].…”

Section: Vulnerable Target Molecules and Cells From The Immune Systemmentioning

confidence: 98%

The Complex Role of Pro- and Anti-Inflammatory Cytokines in Cysticercosis:Immunological Lessons from Experimental and Natural Hosts

Terrazas¹

2008

CTMC

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Parasitic helminthes have developed complex mechanisms to evade or modulate hosts responses. Studies on cysticercosis are solid, but scarce. The most studied immunological models of cysticercosis are Taenia crassiceps infecting mice and T. solium infecting pigs. These parasites, despite being widely exposed to the host, are able to modulate the host immune system. Taenia metacestodes, much like other helminthes parasites, have developed complicated strategies in order to infect and successfully colonize their hosts. We focus here on the accumulated evidence from experimental models that have been helpful in analyzing and characterizing the host immune response to cysticercosis. Moreover, the mouse model has been used to design rationale vaccine strategies, some of them with promising results. We also discuss recent advances in understanding immune-regulation of cysticercosis. The parasite is able to manipulate the host immune system into supporting its survival by keeping a low inflammatory profile by causing the production of some cysticerci-released products that have immunomodulatory activities, as well as promoting the raise of alternatively activated macrophages. Finally, we delineate, according to recent literature, the likely pathway involved in protection and susceptibility against cysticercosis. As more aspects of the role of different immune and parasite-derived molecules are elucidated, better therapeutic targets may be identified to help treat cysticercosis.

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“…Carbohydrates present in soluble antigens of this parasite are responsible for Th2 polarization in vivo [132] and induction of myeloid suppressor innate cells [133]. We have found that DC exposure to excreted/secreted products of high molecular weight from T. crassiceps metacestodes (TcES) induce an increment in MHCII expression but not in the costimulatory molecules CD80, CD86 or CD40 or in cytokine production.…”

Section: Helminth Parasitesmentioning

confidence: 99%

Modulation of Dendritic Cell Responses by Parasites: A Common Strategy to Survive

Terrazas

Gómez-García

2010

Journal of Biomedicine and Biotechnology

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Parasitic infections are one of the most important causes of morbidity and mortality in our planet and the immune responses triggered by these organisms are critical to determine their outcome. Dendritic cells are key elements for the development of immunity against parasites; they control the responses required to eliminate these pathogens while maintaining host homeostasis. However, there is evidence showing that parasites can influence and regulate dendritic cell function in order to promote a more permissive environment for their survival. In this review we will focus on the strategies protozoan and helminth parasites have developed to interfere with dendritic cell activities as well as in the possible mechanisms involved.

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Carbohydrate components of Taenia crassiceps metacestodes display Th2-adjuvant and anti-inflammatory properties when co-injected with bystander antigen

Cited by 42 publications

References 50 publications

Extracts of the Rat Tapeworm,Hymenolepis diminuta, Suppress Macrophage ActivationIn Vitroand Alleviate Chemically Induced Colitis in Mice

Extracts of the Rat Tapeworm,Hymenolepis diminuta, Suppress Macrophage ActivationIn Vitroand Alleviate Chemically Induced Colitis in Mice

The Complex Role of Pro- and Anti-Inflammatory Cytokines in Cysticercosis:Immunological Lessons from Experimental and Natural Hosts

Modulation of Dendritic Cell Responses by Parasites: A Common Strategy to Survive

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