Modulation of Dendritic Cell Responses by Parasites: A Common Strategy to Survive

Terrazas, César; Terrazas, Luis I.; Gómez-García, Lorena

doi:10.1155/2010/357106

Cited by 41 publications

(46 citation statements)

References 155 publications

(241 reference statements)

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“…DC are central regulators of innate and adaptive immunity against different kinds of pathogens [35-37]. Based on different developmental pathways, surface markers and tissue localizations, DC can be divided into distinct subsets exhibiting specific functions [38].…”

Section: Discussionmentioning

confidence: 99%

Modulation of respiratory dendritic cells during Klebsiella pneumonia infection

Hackstein

Kranz

Lippitsch

et al. 2013

Respiratory Research

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BackgroundKlebsiella pneumoniae is a leading cause of severe hospital-acquired respiratory tract infections and death but little is known regarding the modulation of respiratory dendritic cell (DC) subsets. Plasmacytoid DC (pDC) are specialized type 1 interferon producing cells and considered to be classical mediators of antiviral immunity.MethodBy using multiparameter flow cytometry analysis we have analysed the modulation of respiratory DC subsets after intratracheal Klebsiella pneumonia infection.ResultsData indicate that pDCs and MoDC were markedly elevated in the post acute pneumonia phase when compared to mock-infected controls. Analysis of draining mediastinal lymph nodes revealed a rapid increase of activated CD103+ DC, CD11b+ DC and MoDC within 48 h post infection. Lung pDC identification during bacterial pneumonia was confirmed by extended phenotyping for 120G8, mPDCA-1 and Siglec-H expression and by demonstration of high Interferon-alpha producing capacity after cell sorting. Cytokine expression analysis of ex vivo-sorted respiratory DC subpopulations from infected animals revealed elevated Interferon-alpha in pDC, elevated IFN-gamma, IL-4 and IL-13 in CD103+ DC and IL-19 and IL-12p35 in CD11b+ DC subsets in comparison to CD11c+ MHC-class IIlow cells indicating distinct functional roles. Antigen-specific naive CD4+ T cell stimulatory capacity of purified respiratory DC subsets was analysed in a model system with purified ovalbumin T cell receptor transgenic naive CD4+ responder T cells and respiratory DC subsets, pulsed with ovalbumin and matured with Klebsiella pneumoniae lysate. CD103+ DC and CD11b+ DC subsets represented the most potent naive CD4+ T helper cell activators.ConclusionThese results provide novel insight into the activation of respiratory DC subsets during Klebsiella pneumonia infection. The detection of increased respiratory pDC numbers in bacterial pneumonia may indicate possible novel pDC functions with respect to lung repair and regeneration.

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Section: Discussionmentioning

confidence: 99%

Modulation of respiratory dendritic cells during Klebsiella pneumonia infection

Hackstein

Kranz

Lippitsch

et al. 2013

Respiratory Research

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“…A fully mature DC is capable to induce T cell activation, proliferation, and differentiation into the Th1 phenotype, whereas an iDC drives Th2 differentiation and induces an impaired proliferative response in T cells [71]. Recently, we have found that the in vitro exposure of murine [64] and human [72] DCs to TcES impairs their maturation.…”

Section: Immunoregulation By T Crassiceps Antigensmentioning

confidence: 99%

Immunoregulation byTaenia crassicepsand Its Antigens

Peón

Espinoza-Jiménez

Terrazas

2013

BioMed Research International

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Taenia crassiceps is a cestode parasite of rodents (in its larval stage) and canids (in its adult stage) that can also parasitize immunocompromised humans. We have studied the immune response elicited by this helminth and its antigens in mice and human cells, and have discovered that they have a strong capacity to induce chronic Th2-type responses that are primarily characterized by high levels of Th2 cytokines, low proliferative responses in lymphocytes, an immature and LPS-tolerogenic profile in dendritic cells, the recruitment of myeloid-derived suppressor cells and, specially, alternatively activated macrophages. We also have utilized the immunoregulatory capabilities of this helminth to successfully modulate autoimmune responses and the outcome of other infectious diseases. In the present paper, we review the work of others and ourselves with regard to the immune response induced by T. crassiceps and its antigens, and we compare the advances in our understanding of this parasitic infection model with the knowledge that has been obtained from other selected models.

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“…VL patients can die from secondary infections or liver damage (Herwaldt, 1999). Leishmania infects and survives inside dendritic cells, neutrophils and macrophages thereby modulating their activation (Gupta et al, 2013; Terrazas et al, 2010). The interaction between parasites and host cells is critical in understanding how the parasite survives inside the phagocyte and prevents its elimination.…”

Section: Introductionmentioning

confidence: 99%

“…Hijacking of intracellular signaling by Leishmania parasites has been studied by measuring phosphorylation and nuclear translocation of key molecules involved in the inflammatory pathway such as STAT1, NFκB and MAPK mainly by using Western blots (Gupta et al, 2013; Terrazas et al, 2010). However total lysates from infected and uninfected macrophages are usually used, lacking the ability to distinguish between infected or bystander macrophage responses.…”

Section: Introductionmentioning

confidence: 99%

Uncovering Leishmania–macrophage interplay using imaging flow cytometry

Terrazas

Oghumu

Martínez-Saucedo

et al. 2015

Journal of Immunological Methods

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Host–pathogen interaction is an area of considerable interest. Intracellular parasites such as Leishmania reside inside phagocytes such as macrophages, dendritic cells and neutrophils. Macrophages can be activated by cytokines such as IFN-γ and Toll like receptor (TLR) agonists resulting in enhanced microbicidal activity. Leishmania parasites hijack the microbicidal function of macrophages, mainly by interfering with intracellular signaling initiated by IFN-γ and TLR ligands. Here we used transgenic Leishmania donovani parasites expressing the red fluorescent protein DsRed2 and imaging-flow cytometry technology to evaluate parasitic loads inside the macrophage in vitro. Further, this methodology enables us to visualize impairment in NFκB translocation to the nucleus in L. donovani infected macrophages. Additionally we show that uninfected bystander macrophages have a similar impairment in NFκB translocation as in L. donovani infected macrophages in response to the TLR4 agonist LPS. This evidence suggests a possible immunosuppressive role for infected macrophages in regulating the activation of uninfected bystander macrophages.

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Modulation of Dendritic Cell Responses by Parasites: A Common Strategy to Survive

Cited by 41 publications

References 155 publications

Modulation of respiratory dendritic cells during Klebsiella pneumonia infection

Modulation of respiratory dendritic cells during Klebsiella pneumonia infection

Immunoregulation byTaenia crassicepsand Its Antigens

Uncovering Leishmania–macrophage interplay using imaging flow cytometry

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