Carbohydrate analysis of water-soluble uronic acid-containing polysaccharides with high-performance anion-exchange chromatography using methanolysis combined with TFA hydrolysis is superior to four other methods

Ruiter, G.A. de; Schols, Henk A.; Voragen, A.G.J.; Rombouts, F.M.

doi:10.1016/0003-2697(92)90520-h

Cited by 297 publications

(151 citation statements)

References 38 publications

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“…The monosaccharide analysis protocol was adapted from the methods of De Ruiter et al (1992) and Wang et al (2013). In brief, 1 mg of dry cell wall powder and 500 mL of 3 N methanolic hydrochloric acid were mixed and incubated at 80°C for 16 h, cooled on ice, and dried with filtered air.…”

Section: Cell Wall Composition Analysismentioning

confidence: 99%

Gradients in Wall Mechanics and Polysaccharides along Growing Inflorescence Stems

et al. 2017

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At early stages of Arabidopsis (Arabidopsis thaliana) flowering, the inflorescence stem undergoes rapid growth, with elongation occurring predominantly in the apical ;4 cm of the stem. We measured the spatial gradients for elongation rate, osmotic pressure, cell wall thickness, and wall mechanical compliances and coupled these macroscopic measurements with molecular-level characterization of the polysaccharide composition, mobility, hydration, and intermolecular interactions of the inflorescence cell wall using solid-state nuclear magnetic resonance spectroscopy and small-angle neutron scattering. Force-extension curves revealed a gradient, from high to low, in the plastic and elastic compliances of cell walls along the elongation zone, but plots of growth rate versus wall compliances were strikingly nonlinear. Neutron-scattering curves showed only subtle changes in wall structure, including a slight increase in cellulose microfibril alignment along the growing stem. In contrast, solid-state nuclear magnetic resonance spectra showed substantial decreases in pectin amount, esterification, branching, hydration, and mobility in an apical-to-basal pattern, while the cellulose content increased modestly. These results suggest that pectin structural changes are connected with increases in pectin-cellulose interaction and reductions in wall compliances along the apical-to-basal gradient in growth rate. These pectin structural changes may lessen the ability of the cell wall to undergo stress relaxation and irreversible expansion (e.g. induced by expansins), thus contributing to the growth kinematics of the growing stem.

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Section: Cell Wall Composition Analysismentioning

confidence: 99%

Gradients in Wall Mechanics and Polysaccharides along Growing Inflorescence Stems

et al. 2017

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“…Neutral sugars were identified and estimated quantitatively by acid hydrolysis of the polysaccharide [25], followed by conversion of the products to alditol acetates and their separation and identification by gas chromatographic (GC) analysis [26]. About 50 mg of lyophilized material was weighed into a glass tube, 3 mL of 1.25 mol/L chlorhydric acid in methanol was added, the tube was sealed and the mixture was heated at 100˚C during 3 h, ice cooled and evaporated at reduced pressure (35˚C).…”

Section: Analysis Of Monosaccharide Compositionmentioning

confidence: 99%

Optimization of Production and Preliminary Characterization of New Exopolysaccharides from <i>Gluconacetobacter hansenii</i> LMG1524

Valepyn¹,

Berezina²,

Paquot³

2012

AiM

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The influence of different carbon and nitrogen sources, of ethanol concentration, the optimal pH, temperature and medium composition were evaluated on extracellular polysaccharides (EPS) synthesis and bacterial growth of Gluconacetobacter hansenii LMG1524, and preliminary characterization of EPS was investigated. The highest EPS yields were obtained using glycerol and ammonium sulphate as carbon and nitrogen sources, respectively. The increase of ethanol concentration in the medium did not influence the EPS synthesis but reduced the bacterial growth. The optimum temperature and pH for polysaccharides production were respectively 25˚C and 5; whereas for cell growth were respectively 30˚C and 4. The optimal culture medium composition was determined as follows: 10 g/L sucrose, 0.892 g/L (NH 4 ) 2 SO 4 , 0.34 g/L NaNO 3 , 3 mL acetic acid, 1.5 g/L KH 2 PO 4 , 1.5 g/L K 2 HPO 4 and 0.6 g/L MgSO 4 . The polysaccharides produced were of 14 and 10 polymerization degrees (DP) and constituted mainly of glucose, galactose and mannose, in relative percent of 36.36, 33.94 and 22.42, respectively.

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“…For sugar analysis the active compound was hydrolysed by heating at 120 • C for 75 min in 2 mol L −1 trifluoroacetic acid (TFA). 15 Released neutral sugars were determined by comparing retention times with those of standards using a high-performance anion exchange chromatograph (DIONEX, Sunnyvale, CA, USA) equipped with a pulsed amperometric detector and a Carbo Pac PA1 column (250 mm × 4 mm). The mobile phase was water and the flow rate was 1 mL min −1 .…”

Section: Extraction and Isolation Of Eriocitrinmentioning

confidence: 99%

Isolation of eriocitrin (eriodictyol 7‐O‐rutinoside) as an arachidonate lipoxygenase inhibitor from Lumie fruit (Citrus lumia) and its distribution in Citrus species

et al. 2006

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Carbohydrate analysis of water-soluble uronic acid-containing polysaccharides with high-performance anion-exchange chromatography using methanolysis combined with TFA hydrolysis is superior to four other methods

Cited by 297 publications

References 38 publications

Gradients in Wall Mechanics and Polysaccharides along Growing Inflorescence Stems

Gradients in Wall Mechanics and Polysaccharides along Growing Inflorescence Stems

Optimization of Production and Preliminary Characterization of New Exopolysaccharides from <i>Gluconacetobacter hansenii</i> LMG1524

Isolation of eriocitrin (eriodictyol 7‐O‐rutinoside) as an arachidonate lipoxygenase inhibitor from Lumie fruit (Citrus lumia) and its distribution in Citrus species

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Carbohydrate analysis of water-soluble uronic acid-containing polysaccharides with high-performance anion-exchange chromatography using methanolysis combined with TFA hydrolysis is superior to four other methods

Cited by 297 publications

References 38 publications

Gradients in Wall Mechanics and Polysaccharides along Growing Inflorescence Stems

Gradients in Wall Mechanics and Polysaccharides along Growing Inflorescence Stems

Optimization of Production and Preliminary Characterization of New Exopolysaccharides from &lt;i&gt;Gluconacetobacter hansenii&lt;/i&gt; LMG1524

Isolation of eriocitrin (eriodictyol 7‐O‐rutinoside) as an arachidonate lipoxygenase inhibitor from Lumie fruit (Citrus lumia) and its distribution in Citrus species

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Optimization of Production and Preliminary Characterization of New Exopolysaccharides from <i>Gluconacetobacter hansenii</i> LMG1524