Carbofuran-Induced Alterations in Body Morphometrics and Histopathology of Liver and Kidneys in the Swiss Albino Mice Mus Musculus L.

Islam, Md. Shafiqul; Mohanta, Moni Krishno; Saha, Ananda Kumar; Mondol, Anup; Hoque, M.M.; Roy, Arun K.

doi:10.12983/ijsres-2014-p0308-0322

Cited by 5 publications

(5 citation statements)

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“…Alterations ranging from moderate degenerative (++) to necrotic were ascertained in group 2. Findings observed in this study were in agreement with those observed in CYP toxicity studies (2,5,8,27). The role of CB2 in liver diseases is controversial.…”

Section: Discussionsupporting

confidence: 91%

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Cannabinoid receptor type 2 agonist JWH-133 deteriorates the liver toxicity induced by cypermethrin

2018

Turk J Vet Anim Sci

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IntroductionCYP is a synthetic type II pyrethroid insecticide widely used for the control of ectoparasites of domestic animals and home pest control owing to its high insecticidal potential and lower mammalian toxicity (1). CYP shows its effect via the voltage-dependent Na + and Ca 2+ channels in the membrane of nerve cells (2-4). There is an increasing amount of evidence suggesting toxic effects on nontarget organisms in chronic exposure of CYP (5,6). Several studies have shown that CYP causes oxidative stress, biochemical changes such as increase in activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and lactate dehydrogenase (LDH); elevation in levels of thiobarbituric acid reactive substances (TBARS), a decrease in activities of the antioxidant enzymes (7,8), and histopathological changes such as vacuolar degeneration, enlarged sinusoid, vacuole formation in hepatocytes, pleomorphism in the nucleus, congestion, necrosis, hepatocellular hypertrophy, multinucleated hepatocytes, mononuclear cell infiltration (MCI), and increase in Kupffer cell numbers (2,6).Marijuana or cannabis, whose main active ingredient is delta-9-tetrahydrocannabinol, activates two G proteincoupled membrane receptors, named CB1 and CB2 (9-11). CB1 is mainly expressed in the nervous system and is less expressed in various peripheral tissues such as the heart, gut, liver, and in cells such as endothelial cells and adipocytes. In comparison, CB2 is largely expressed in immune cells, tonsils, spleen, and testis. Moreover, it has been observed to be expressed to a lesser extent in other tissues and in cells like hepatic myofibroblasts (12).Recent studies have shown the dysregulation of endocannabinoid system in experimental models of bowel, liver, and heart diseases. Evidence suggests that Abstract: Cannabinoid receptor 2 (CB2) has a role in the pathology of some liver diseases. Cypermethrin (CYP) is a synthetic pyrethroid insecticide used commonly for the control of pests and vectors, and for protection of foodstuffs. In this study, the involvement of CB2 in CYP-induced liver damage in rats was investigated. CYP was applied to rats orally at a dose of 125 mg/kg bw per day for 14 days. CB2 agonist JWH-133 was administered by intraperitoneal injection at a dose of 3 mg/kg bw per day for the last 4 days of CYP toxicity. JWH-133 deteriorated the liver damage induced by CYP. JWH-133 increased serum hepatic enzyme activities (aspartate aminotransferase, alanine aminotransferase) in rats given CYP. However, it decreased lipid peroxidation. There were no differences in the levels of hepatic CB2 mRNA among the groups. CB2 receptors were expressed in a small amount in normal liver tissue. In contrast, the expression levels of CB2 in CYPtreated rats were increased in fibrocyte/fibroblast, bile duct epithelial cells, Kupffer cells, and mast cells. In conclusion, CB2 was upregulated in the liver exposed to CYP, predominantly in hepatic fibrogenic cells. JWH-133 enhanced the CYPinduced liver damage by receptor mediated and/or non...

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Section: Discussionsupporting

confidence: 91%

“…In group 4, mild (+) parenchymal degeneration was observed. (5,27,28). Group 3 had less weight gain among the groups.…”

Section: Discussionmentioning

confidence: 88%

Cannabinoid receptor type 2 agonist JWH-133 deteriorates the liver toxicity induced by cypermethrin

2018

Turk J Vet Anim Sci

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“…However, the kidney of group A showed severe hemorrhages, necrosis along with occlusion of fluid in renal tubules lumen, and accumulation of inflammatory cells in renal spaces resulted in interstitial nephritis (Figure 1e). Similar renal abnormalities were also reported in albino mice (Islam et al, 2014), tilapia fish (Hamed et al, 2021), broiler chicks (Ali et al, 2020), and rats (Datta et al, 2021), while the literature is lacking in evidence about CF intoxication in cattle egret.…”

Section: Histopathological Examinationssupporting

confidence: 59%

Sublethal toxicity of carbofuran in cattle egret (Bubulcus ibis coromandus): hematological, biochemical, and histopathological alterations

et al. 2024

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This study was aimed to investigate Carbofuran (CF)-induced pathological changes in cattle egret. Two hundred cattle egrets were reared and equally divided into four groups and given different CF concentrations (0.03 mg/L, 0.02 mg/L, 0.01 mg/L and 0 mg/L (control group)). Hematology, serum biochemistry, histopathology, and immunological markers were studied. Our results confirm that CF induces anemic conditions, leukocytosis, elevated liver enzymatic activity, and alterations in renal biomarkers. Moreover, specific microscopic lesions such as multifocal necrosis, pyknotic nuclei, hemorrhages, congestion, and inflammatory cell proliferation were observed in the liver, kidney, spleen, and thymus. These findings suggest that CF can induce harmful effects, so the application of this pesticide in the field must be strictly monitored to mitigate the possibility of exposure to non-target species.

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“…Carbofuran (C 12 H 15 NO 3 : 2,3-dihydro-2,2-dimethyl-1-7-benzofuranyl benzofuranyl N -methyl carbamate) is an important carbamate pesticide that is used in agriculture in several parts of the world . Research findings indicate that frequent use of this pesticide may have toxic effects in the central nervous system, along with the liver, skeletal muscles, kidneys, and heart of even the nonspecified mammalian systems. − Like other hepatotoxic pesticides, chronic exposure to carbofuran can cause necrosis of the hepatocytes via oxidative injuries in the structure and functions of cellular membranes. , Furthermore, carbamates are known acetyl cholinesterase inhibitors that cause reversible carbamylation of the hydroxyl (−OH) group of the serine residue at the active site and increase acetylcholine . In this respect, there is a relationship between augmentation of acetylcholine and the extent of lipid peroxidation as well as cell damage …”

Section: Introductionmentioning

confidence: 99%

Hepatoprotective and Antioxidant Activities of Justicia gendarussa Leaf Extract in Carbofuran-Induced Hepatic Damage in Rats

Mondal

Hossain

Rahman

et al. 2019

Chem. Res. Toxicol.

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In folk medicines, Justicia gendarussa (J. gendarussa) is used as a depurative herb for treating fever, pain, and cancer and as laxative for constipation. The aim of the present investigation was to evaluate the hepatoprotective effect of the leaf methanol extract of J. gendarussa leaf (J gMe) against carbofuran (CF)-intoxicated liver injuries in Sprague−Dawley rats, along with the antioxidant activity of this extract. For this purpose, levels of serum diagnostic markers, hepatic antioxidant enzymes, and liver histoarchitecture were employed to justify the protective efficacy of J gMe. In addition, the phenolic and flavonoid contents of the extract were quantified, and antioxidant activity was investigated using the 1,1-diphenyl-2-picrylhydrazyl (DPPH), nitric oxide, hydrogen peroxide, and hydroxyl free radical scavenging assays. Results revealed that the leaf extract caused a significant (<0.05, <0.01) decrease of the level of hepatic enzymes, triglycerides, and bilirubin and an increase of the total protein. J gMe has also significantly (<0.05, <0.01) lowered the level of malonylaldehyde. Carbofuran markedly suppressed hepatic antioxidant enzymes, however, the leaf extract significantly augmented these enzymes. The hepatoprotective effect was demonstrated by the improvement in the histo-architectural features of liver sections of CFintoxicated rats treated with J gMe at 500 mg/kg dose. In addition, J gMe showed moderate total phenolic and total flavonoid content, whereas the IC 50 values of DPPH, nitric oxide, hydrogen peroxide, and hydroxyl free radical scavenging assays were 71.31 ± 0.42, 134.82 ± 0.14, 47.69 ± 0.38, and 118.44 ± 0.30 μg/mL, respectively. In conclusion, the present study suggests the protective role of J gMe against hepatic injury induced by CF, which may be attributed to its higher antioxidant properties and thereby scientifically justifies its traditional use.

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Carbofuran-Induced Alterations in Body Morphometrics and Histopathology of Liver and Kidneys in the Swiss Albino Mice Mus Musculus L.

Cited by 5 publications

References 12 publications

Cannabinoid receptor type 2 agonist JWH-133 deteriorates the liver toxicity induced by cypermethrin

Cannabinoid receptor type 2 agonist JWH-133 deteriorates the liver toxicity induced by cypermethrin

Sublethal toxicity of carbofuran in cattle egret (Bubulcus ibis coromandus): hematological, biochemical, and histopathological alterations

Hepatoprotective and Antioxidant Activities of Justicia gendarussa Leaf Extract in Carbofuran-Induced Hepatic Damage in Rats

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