Carbodiimide inactivation of matrix metalloproteinases in radicular dentine

Comba, Allegra; Scotti, Nicola; Mazzoni, Annalisa; Maravić, Tatjana; Cunha, Sandra Ribeiro; Tempesta, Riccardo Michelotto; Carossa, Massimo; Pashley, David H.; Tay, Franklin R.; Breschi, Lorenzo

doi:10.1016/j.jdent.2019.01.007

Cited by 17 publications

(23 citation statements)

References 52 publications

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“…Cross-linking of the catalytic or noncatalytic portion of an MMP by EDC disables the recognition and cleavage of the substrate, or unraveling of the triple helix of a collagen molecule (Busenlehner and Armstrong 2005; Mazzoni et al 2017; Mazzoni et al 2018). Such a property has been reported by others directly after bonding and after aging the bonded specimens for 1 y in artificial saliva (Tjäderhane et al 2013; Scheffel et al 2014; Mazzoni et al 2018; Comba et al 2019).…”

Section: Discussionsupporting

confidence: 74%

“…The commercially available synthetic cross-linking reagent 1-ethyl-3(3-dimethylaminopropyl) carbodiimide (EDC) preserves bond strength and inhibits MMPs for up to 12 mo (longest reported aging time so far; Tezvergil-Mutluay et al 2012; Mazzoni et al 2014; Mazzoni et al 2017; Comba et al 2019) as well as cysteine cathepsins (Turco et al 2016). Although collagen fibril cross-linking and MMP inactivation are irreversible processes, studies with longer aging times are required to confirm this hypothesis.…”

Section: Introductionmentioning

confidence: 99%

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Dentin Cross-linking Effect of Carbodiimide After 5 Years

et al. 2021

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Carbodiimide (EDC)–based dentin primers preserve hybrid layer (HL) integrity. However, aging >1 y has not been investigated. The present study examined whether the cross-linking effect of EDC was reflected in dentin bond strength, endogenous enzymatic activity, and the chemical profile of the HL after 5-y aging in artificial saliva. Noncarious human third molars ( N = 42) were cut to expose middle/deep coronal dentin and treated as follows: group 1, dentin etched with 35% H3PO4, pretreated with a 0.3M aqueous EDC primer for 1 min and restored with XP Bond (Dentsply Sirona); group 2, as in group 1 but without EDC pretreatment; group 3, Clearfil SE Bond (Kuraray-Noritake) primer applied to dentin surface, followed by EDC pretreatment as in group 1 and application of bond; group 4, as in group 3 without EDC pretreatment. After composite buildup, the specimens were cut into sticks or slabs, depending on the experiment. All tests were performed at baseline (T0) and after 5 y of aging (T5) in artificial saliva at 37 °C. Microtensile bond strength (µTBS) was tested at a crosshead speed of 1 mm/min until failure. Endogenous enzymatic activity was investigated with in situ zymography. The chemical profile of HL was determined via Raman spectroscopy. Three-way analysis of variance and post hoc Tukey test were used to analyze µTBS and in situ zymography data (α = 0.05). EDC pretreatment and aging significantly influenced µTBS and in situ zymography results ( P < 0.05). Higher bond strength and lower gelatinolytic activity were identified in the EDC-treated groups at T5 ( P < 0.05), especially in the etch-and-rinse groups. Raman spectra revealed less defined amide III peaks in control specimens at T5. The EDC cross-linking effect persisted in the HL for 5 y in terms of bond strength, collagen structure preservation, and dentinal enzyme silencing.

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Section: Discussionsupporting

confidence: 74%

Section: Introductionmentioning

confidence: 99%

Dentin Cross-linking Effect of Carbodiimide After 5 Years

et al. 2021

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“…The demineralized dentin cross-link degree was shown to increase by EDC compared with control and ethanol treatments in a study by Tang et al (2016). In agreement with the current study, Comba et al (2019) reported that the application of EDC did not cause a F I G U R E 1 Error bar of the mean and 95% confidence interval of push-out bond strength (MPa) of fiber posts to radicular dentin before and after thermocycling. CHX, chlorhexidine; CI, confidence interval; DMSO, dimethyl sulfoxide; EDC, carbodiimide.…”

Section: Discussionsupporting

confidence: 89%

Effect of pretreatment with matrix metalloproteinase inhibitors on the durability of bond strength of fiber posts to radicular dentin

Ghazvehi

Saffarpour

Habibzadeh

2022

Clinical & Exp Dental Res

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Objectives Application of matrix metalloproteinases inhibitors has been suggested to improve the durability of resin–dentin bonding. The purpose of this study was to evaluate the effect of dimethyl sulfoxide (DMSO), carbodiimide (EDC), and chlorhexidine (CHX) treatment on the push‐out bond strength of fiber posts to radicular dentin. Materials and Methods 24 extracted premolars were assigned randomly to 4 groups after root canal treatment and post space preparation (n = 6). In the first, second, and third groups, radicular dentin was treated with 1 ml of 5% DMSO, 0.3 M EDC, and 2% CHX, respectively. The fourth group (control) received no treatment. The root canals were primed with ED primer II, and the fiber posts were cemented with Panavia F2.0. In each group, half of the specimens were subjected to the push‐out test and the other half to 3000 thermal cycles before testing. Data were analyzed using two‐way analysis of variance via SPSS version 20 (p < .05). Results Among the nonthermocycled specimens, the values of push‐out bond strength were observed in the control, EDC, CHX, and DMSO groups, in decreasing order. Among the thermocycled specimens, the values were observed in the control, EDC, DMSO, and CHX groups in decreasing order. Thermocycling had a significant adverse effect on the push‐out bond strength (p = .015), but the effect of material (p = .375), and the interaction effect of material and thermocycling (p = .998) were not significant. Conclusions Application of CHX, DMSO, and EDC had no significant effect on the bond strength of fiber posts to radicular dentin.

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“…The use of natural less toxic polymers for dentine pre-treatment, such as Ch, has recently been studied [4,33,34,42]. Previous reports stated that the incorporation [34] of this polymer within an adhesive did not influence the immediate bond strength.…”

Section: Discussionmentioning

confidence: 99%

Effect of Chitosan as a Cross-Linker on Matrix Metalloproteinase Activity and Bond Stability with Different Adhesive Systems

et al. 2020

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The aim of the present study was to evaluate the effect of 0.1% chitosan (Ch) solution as an additional primer on the mechanical durability and enzymatic activity on dentine using an etch-and-rinse (E&R) adhesive and a universal self-etch (SE) adhesive. Microtensile bond strength and interfacial nanoleakage expression of the bonded interfaces for all adhesives (with or without pretreatment with 0.1% Ch solution for 1 min and air-dried for 5 s) were analyzed immediately and after 10,000 thermocycles. Zymograms of protein extracts from human dentine powder incubated with Optibond FL and Scotchbond Universal on untreated or Ch-treated dentine were obtained to examine dentine matrix metalloproteinase (MMP) activities. The use of 0.1% Ch solution as an additional primer in conjunction with the E&R or SE adhesive did not appear to have influenced the immediate bond strength (T0) or bond strength after thermocycling (T1). Zymography showed a reduction in MMP activities only for mineralized and demineralized dentine powder after the application of Ch. Application of 0.1% Ch solution does not increase the longevity of resin–dentine bonds. Nonetheless, the procedure appears to be proficient in reducing dentine MMP activities within groups without adhesive treatments. Further studies are required to comprehend the cross-linking of Ch with dentine collagen.

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Carbodiimide inactivation of matrix metalloproteinases in radicular dentine

Cited by 17 publications

References 52 publications

Dentin Cross-linking Effect of Carbodiimide After 5 Years

Dentin Cross-linking Effect of Carbodiimide After 5 Years

Effect of pretreatment with matrix metalloproteinase inhibitors on the durability of bond strength of fiber posts to radicular dentin

Effect of Chitosan as a Cross-Linker on Matrix Metalloproteinase Activity and Bond Stability with Different Adhesive Systems

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