Carbodiimide cross-linked gelatin: a new coating for porous polyester arterial prostheses

Marois, Yves; Chakfé, Nabil; Deng, Xiaoyan; Marois, M; How, T.V.; King, Martin W.; Guidoin, Robert

doi:10.1016/0142-9612(95)93576-y

Cited by 100 publications

(43 citation statements)

References 23 publications

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“…Gelatin and albumin were used according to their biocompatibility and low surface thrombogenicity. 4,5 A second improvement of the performance of biomaterials in contact with blood was achieved by adsorption or immobilization of physiologically active substances via covalent binding. Among these, heparin has been particularly investigated to obtain bloodcompatible materials.…”

Section: Introductionmentioning

confidence: 99%

Heparin immobilized on proteins usable for arterial prosthesis coating: Growth inhibition of smooth-muscle cells

Laemmel

Penhoat

Warocquier-Clerout

et al. 1998

J. Biomed. Mater. Res.

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Gelatin or a mixture of albumin and gelatin has been proposed for the coating of vascular grafts according to their surface thrombogenicity and biocompatibility, and the possibility of biodegradation. Heparin treatment of hemocompatible surfaces improved the patency of prostheses. In this study, different amounts of heparin were immobilized on these protein gels using a water-soluble carbodiimide [1-ethyl-3-(3-dimethylaminopropyl) carbodiimide]. The results showed a coupling of heparin with gelatin and/or albumin at the surface of the gels, stable for as long as 1 month. From 0.20 to 3.60 microg x cm(-2), heparin could be immobilized. The antiproliferative activity of immobilized heparin was controlled toward bovine smooth-muscle cells grown on these gels. Cell growth inhibition was dose dependent, but the percentages of inhibition were lower at day 8 than at day 4 at any heparin concentration used under experimental conditions. Referring to heparin in solution, immobilized heparin displayed an antiproliferative activity that improved the potential interest for coating.

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Section: Introductionmentioning

confidence: 99%

Heparin immobilized on proteins usable for arterial prosthesis coating: Growth inhibition of smooth-muscle cells

Laemmel

Penhoat

Warocquier-Clerout

et al. 1998

J. Biomed. Mater. Res.

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“…28 In contrast to conventional chemical agents such as glutaraldehyde or polyepoxides, carbodiimides do not remain as a part of linkage but simply change to water-soluble urea derivatives that have very low cytotoxicity. 41 If stable covalent linkages are produced, this method can preclude depolymerization and release of residual toxic reagent.…”

Section: Cell Culture Experimentsmentioning

confidence: 99%

Bacterial cellulose-collagen nanocomposite for bone tissue engineering

et al. 2012

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A nanocomposite based on bacterial cellulose (BC) and type I collagen (COL) was evaluated for in vitro bone regeneration. BC membranes were modified by glycine esterification followed by cross-linking of type I collagen employing 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide. Collagen incorporation was studied by spectroscopy analysis. X-Ray diffraction showed changes in the BC crystallinity after collagen incorporation. The elastic modulus and tensile strength for BC-COL decreased, while the strain at failure showed a slight increase, even after sterilization, as compared to pristine BC. Swelling tests and contact angle measurements were also performed. Cell culture experiments performed with osteogenic cells were obtained by enzymatic digestion of newborn rat calvarium revealed similar features of cell morphology for cultures grown on both membranes. Cell viability/proliferation was not different between BC and BC-COL membranes at day 10 and 14. The high total protein content and ALP activity at day 17 in cells cultured on BC-COL indicate that this composite allowed the development of the osteoblastic phenotype in vitro. Thus, BC-COL should be considered as alternative biomaterial for bone tissue engineering.

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“…There are generally two types of gelatin: Type A and Type B. Gelatin Type A is extracted and processed by acidic pretreatment of collagen, whereas gelatin Type B is obtained by alkaline pretreatment. The alkaline pretreatment converts glutamine and asparagine residues into glutamic and aspartic acids, respectively, which leads to higher carboxylic acid content for gelatin Type B than for gelatin Type A. Gelatin has several potential advantages over other natural proteins, such as its biological origin, biodegradability and commercial availability at low cost [24][25][26][27][28][29][30][31][32][33].…”

Section: Introductionmentioning

confidence: 99%

Three-dimensional electrospun ECM-based hybrid scaffolds for cardiovascular tissue engineering

et al. 2008

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Electrospinning using natural proteins or synthetic polymers is a promising technique for the fabrication of fibrous scaffolds for various tissue engineering applications. However, one limitation of scaffolds electrospun from natural proteins is the need to cross-link with glutaraldehyde for stability, which has been postulated to lead to many complications in vivo including graft failure. In this study, we determined the characteristics of hybrid scaffolds composed of natural proteins including collagen and elastin, as well as gelatin, and the synthetic polymer poly(ε-caprolactone) (PCL), so to avoid chemical cross-linking. Fiber size increased proportionally with increasing protein and polymer concentrations, whereas pore size decreased. Electrospun gelatin/PCL scaffolds showed a higher tensile strength when compared to collagen/elastin/PCL constructs. To determine the effects of pore size on cell attachment and migration, both hybrid scaffolds were seeded with adipose-derived stem cells. Scanning electron microscopy and nuclei staining of cell-seeded scaffolds demonstrated complete cell attachment to the surfaces of both hybrid scaffolds, although cell migration into the scaffold was predominantly seen in the gelatin/PCL hybrid. The combination of natural proteins and synthetic polymers to create electrospun fibrous structures resulted in scaffolds with favorable mechanical and biological properties.

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Carbodiimide cross-linked gelatin: a new coating for porous polyester arterial prostheses

Cited by 100 publications

References 23 publications

Heparin immobilized on proteins usable for arterial prosthesis coating: Growth inhibition of smooth-muscle cells

Heparin immobilized on proteins usable for arterial prosthesis coating: Growth inhibition of smooth-muscle cells

Bacterial cellulose-collagen nanocomposite for bone tissue engineering

Three-dimensional electrospun ECM-based hybrid scaffolds for cardiovascular tissue engineering

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