Carbapenem-resistant bacteria in a secondary wastewater treatment plant

Hrenović, Jasna; Ganjto, Marin; Goić-Barišić, Ivana

doi:10.4314/wsa.v43i2.02

Cited by 17 publications

(14 citation statements)

References 15 publications

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“…All the colonies that grew on the plates were labelled CRB and labelled as CRB37 or CRB42. Referencing to our previous work (Hrenovic et al 2017a(Hrenovic et al , 2017c, the CRB37 are considered to be natively present and intrinsically carbapenem-resistant while CRB42 are considered to be of anthropogenic origin, and potentially clinically relevant, carrying acquired resistance.…”

Section: Bacteriological Analysismentioning

confidence: 91%

“…The CRB were cultivated by placing membrane filters on selective CHROMagar™ Acinetobacter medium (CHROMagar, Paris, France): agar: 15 g L −1 ; peptone and yeast extract: 12 g L −1 ; salts: 4 g L −1 ; chromogenic mix: 1.8 g L −1 , supplemented with CR102 (one dose per litre) (CHROMagar 2018). For each sample, two incubation temperatures were used, 37 and 42°C (Hrenovic et al 2017a(Hrenovic et al , 2017c. All the colonies that grew on the plates were labelled CRB and labelled as CRB37 or CRB42.…”

Section: Bacteriological Analysismentioning

confidence: 99%

“…To do that, we used a novel method that would be able to distinguish between native, intrinsically resistant soil bacteria and those that are presumably of anthropogenic origin, therefore clinically relevant. This methodology has been successfully used in our previous studies where CRB were monitored in wastewater treatment plant (Hrenovic et al 2017a(Hrenovic et al , 2017c) and now it was, to best of our knowledge, implemented for soil samples for the first time. This methodology is based on cultivation of environmental samples on 37 and 42°C in parallel.…”

Section: Introductionmentioning

confidence: 99%

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Presence of carbapenem-resistant bacteria in soils affected by illegal waste dumps

Hrenović

Ivanković

Durn

et al. 2018

International Journal of Environmental Health Research

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The carbapenem-resistant bacteria (CRB) are currently at the top of the WHO priority list of bacteria that pose the greatest threat to human health. Considering that soil is one of the important environments for the emergence of antibiotic-resistant bacteria, we isolated and quantified cultivable CRB in soils across Croatia, including ones affected by illegal dumps. We cultivated CRB at two temperatures, distinguishing between the intrinsically resistant CRB (37°C, mostly Stenotrophomonas spp.) and the ones that are presumably human-associated and clinically relevant (42°C, Acinetobacter sp., Enterobacteriaceae, Burkholderia spp.). Our study demonstrated that distinguishing between the two offers a better insight into the diversity of CRB in the environment. The ones cultivated at 37°C were found in almost all soil samples, while the presumably clinically relevant ones were absent from uncontaminated pasture and grassland, indicating that human-associated CRB are unlikely to be found in soils spared from anthropogenic influence.

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Section: Bacteriological Analysismentioning

confidence: 91%

Section: Bacteriological Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Presence of carbapenem-resistant bacteria in soils affected by illegal waste dumps

Hrenović

Ivanković

Durn

et al. 2018

International Journal of Environmental Health Research

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“…Incubation at 42°C suppresses the growth of environmental autochthonous species with intrinsic resistance to carbapenems. [13][14][15] Total heterotrophic bacteria, intestinal enterococci, and carbapenem-resistant bacterial counts were determined as colony forming units (CFUs), logarithmically transformed and expressed as log CFU per 1 mL of manure. The prevalence of carbapenem-resistant bacteria among total heterotrophic bacteria was calculated as (log CFU/mL carbapenem-resistant /log CFU/mL heterotrophic ) • 100.…”

Section: Bacteriological Analysesmentioning

confidence: 99%

Carbapenem-Resistant Acinetobacter baumannii Recovered from Swine Manure

Hrenović

Musić

Durn

et al. 2019

Microbial Drug Resistance

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Recently, concerns have been raised about the possibility of Acinetobacter baumannii transmission between animals and humans. So far, A. baumannii has been reported in animals with which people can come into contact. The presence of this pathogen in animal manure presents an equally important public health risk. In this study, we report the finding of two A. baumannii isolates in swine manure from a Croatian pig farm. Both isolates shared features with the widespread human clinical isolates: affiliation to the international clonal lineage 2 (ST-195), carbapenem, and extensive drug resistance and the plasmid-located acquired bla OXA-23 gene. These two A. baumannii isolates survived anaerobic conditions, competition with other microorganisms, and elevated concentrations of heavy metals in the stagnant swine manure for at least 2 weeks. These findings call for bacteriological analysis and disinfection of liquid swine manure before its application as a fertilizer in traditional extensive agriculture.

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“…Rise in fluoroquinolone resistance among Enterobacteriaceae causing community acquired or healthcare associated urinary tract infections and intraabdominal infections, exceeding 50% was also reported in some parts of the world, particularly in Asia [10]. Resistance to imipenem and other carbapenems have also been studied globally among clinical bacterial isolates [11,12]. Mechanism behind antimicrobial resistance in these microorganism includes production of extended spectrum ß lactamases (ESBL) and metallo ß lactamase (MBL), changes in membrane permeability, over-expression of efflux pump and production of biofilms etc.…”

Section: Introductionmentioning

confidence: 99%

Prevalence and Susceptibility Analysis of Gram Negative Pathogens in Super Specialty Tertiary Care Centers, Pune in India from January 2018- January 2019

Satras¹

2019

MRJI

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Background and Objective: The encumbrance of antimicrobial resistance worldwide is substantive and likely to rise without any appropriate treatment which has enhanced the urge for the development of either new antibiotics or adjuvant therapy with antibiotics. Thus, we aimed to study a comparative antibiogram pattern of 758 clinical isolates collected from Pawana Hospital and Accord SDH, Pune (India), towards Elores (a novel antibiotic and adjutant entity of ceftriaxone, sulbactam and disodium edetate) and other antibiotics (imipenem, meropenem and piperacillin + tazobactam). Methods: The clinical samples collected from outpatients and inpatients during a period of one year (January, 2018 to January, 2019), from Pawana Hospital and Accord SDH, Pune (India) and were further subjected to bacterial identification. Antibiotic susceptibility testing was executed in accordance with the recommendations of Clinical Laboratory Standards Institute (CLSI) guidelines. Results: Out of 758 collected samples, urine samples contributed 69.41 and 37.63% among Enterobacteriaceae and Non-Enterobacteriaceae followed by pus (20.25 and 19.35%) and sputum (4.2 and 19.89%) while <4% and <12% Enterobacteriaceae and Non-Enterobacteriaceae isolates were collected from rest of the specimen. E. coli were found most prevalent (50.13%) along with 19.13 and 16.09% prevalence of Pseudomonas spp. and Klebsiella spp. whereas rest of the pathogens were <7% present. The antibacterial activity of Elores (87.80%) was observed superior to carbapenem drugs (meropenem; 62.67% and imipenem; 60.95%) and far better to piperacillin + tazobactam (48.42%) against 758 clinical pathogens. Antibiogram profile depicted Elores as most susceptible (95.10%) drug towards Enterobacteriaceae isolates which was approximately 31-50% more sensitive than other test drugs. Similar pattern was obtained for Non-enterobacteriaceae isolates (62.90%) where Elores contributed approximately 1-5% higher activity. However, Elores also conquered 79.51-86.59% resistance among meropenem, imipenem and piperacillin + tazobactam resistant pathogens. Conclusion: Susceptibility profile data revealed the equivalence of Elores (Antibiotic-adjuvant entity; AAE) with carbapenem drugs (meropenem and imipenem) and superiority over piperacillin + tazobactam against clinical pathogens. Elores was also found active towards meropenem, imipenem and piperacillin + tazobactam resistant pathogens. Therefore, Elores, a resistance breaker, can be used as an efficient treatment alternate towards infections caused by resistant pathogens.

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Carbapenem-resistant bacteria in a secondary wastewater treatment plant

Cited by 17 publications

References 15 publications

Presence of carbapenem-resistant bacteria in soils affected by illegal waste dumps

Presence of carbapenem-resistant bacteria in soils affected by illegal waste dumps

Carbapenem-Resistant Acinetobacter baumannii Recovered from Swine Manure

Prevalence and Susceptibility Analysis of Gram Negative Pathogens in Super Specialty Tertiary Care Centers, Pune in India from January 2018- January 2019

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