Caracterização de isolados de Colletotrichum lagenarium de pepino com base em marcadores isoenzimáticos

Rios, Patrícia R.P.; Silveira, Elineide Barbosa da; Martins, Luiza Suely Semem; Neto, Edson B. Silva; Gomes, Andréa M.A.

doi:10.1590/s0102-05362004000400012

Cited by 2 publications

(1 citation statement)

References 8 publications

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“…Lima Filho et al (2003), using EST and ACP enzyme patterns obtained by PAGE, reported intraspecific variability among C. gloeosporioides isolates from several tropical fruits. Additionally, Rios et al (2004) successfully used the EST, ACP, ALP and MDH isoenzymes, to confirm the existence of genetic variability in C. lagenarium.…”

Section: A B Cmentioning

confidence: 99%

Isoenzymatic characterization of Colletotrichum kahawae isolates with different levels of aggressiveness

Loureiro

Guerra‐Guimarães

Lidon

et al. 2011

Trop. plant pathol.

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The hemibiotrophic fungus Colletotrichum kahawae is the causal agent of Coffee Berry Disease -CBD, an economically devastating disease restricted to Arabica coffee production in Africa. Understanding pathogen variability is crucial for the implementation of disease control measures. In this study, six isoenzymatic systems (esterase, acid and alkaline phosphatase, malate dehydrogenase, peroxidase and superoxide dismutase) were used to assess the genetic variation among 12 C. kahawae isolates (from different geographic origins and with different levels of aggressiveness towards coffee) and one Colletotrichum gloeosporioides isolate (not pathogenic to green berries). Cluster analysis of the banding profiles obtained for the six enzymes enabled the differentiation of the two species and revealed the existence of intraspecific variability among C. kahawae isolates. Alkaline phosphatase was the most discriminative enzyme, allowing also the discrimination between the most and least aggressive isolates of C. kahawae.

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Section: A B Cmentioning

confidence: 99%

Isoenzymatic characterization of Colletotrichum kahawae isolates with different levels of aggressiveness

Loureiro

Guerra‐Guimarães

Lidon

et al. 2011

Trop. plant pathol.

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Promoção de crescimento e indução de resistência à antracnose por Trichoderma spp. em pepineiro

Silva¹,

Guzzo²,

Lucon³

et al. 2011

Pesq. agropec. bras.

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Resumo -O objetivo deste trabalho foi determinar o efeito de 60 isolados de Trichoderma e do produto Trichodermil na promoção do crescimento e na indução de resistência sistêmica à antracnose, causada por Colletotrichum lagenarium em pepineiro, além de identificar as espécies dos isolados de Trichoderma spp. efetivas como indutores de resistência. Nos experimentos de promoção de crescimento, os isolados de Trichoderma spp. foram submetidos à inoculação no substrato e, após 21 dias, a massa de matéria seca da parte aérea das plantas foi mensurada. Nos experimentos de indução de resistência, os isolados que promoveram crescimento foram introduzidos no substrato, na base das plantas, sete dias antes da inoculação de C. lagenarium nas folhas. O isolado que apresentou melhor desempenho foi avaliado quanto à redução dos sintomas de antracnose, em aplicações aos 3, 7 ou 14 dias antes da inoculação do patógeno, e quanto à capacidade de aumentar a atividade de peroxidase. Dezenove isolados e o Trichodermil promoveram o crescimento de pepineiro em até 100% e conferiram proteção à antracnose em até 88,39%. O isolado IB 31/06 reduziu a severidade da doença nos intervalos de tempo avaliados. Não foi observado aumento significativo de peroxidase, sete dias após o tratamento com IB 31/06, nas plantas tratadas e infectadas com o patógeno, em comparação às plantas infectadas. O sequenciamento gênico dos dezenove isolados permitiu a identificação de sete espécies distintas de Trichoderma.Termos para indexação: Colletotrichum lagenarium, Cucumis sativus, antagonismo, biocontrole, indução de proteção sistêmica, peroxidase. Growth promotion and resistance induction against anthracnosein cucumber using Trichoderma spp.Abstract -The objective of this work was to determine the effect of 60 isolates of Trichoderma and the product Trichodermil on the growth promotion and on the induction of systemic resistance to anthracnose caused by Colletotrichum lagenarium in cucumber, and to identify isolates of Trichoderma spp. effective as resistance inductors. In the assays of growth promotion, the Trichoderma spp. isolates were inoculated in the substrate and, after 21 days, the shoot dry weight of the plants was measured. In the experiments of resistance induction, the isolates which promoted growth were inoculated in the substrate, at the base of the plants, seven days before C. lagenarium inoculation in the leaves. The isolate which showed the best performance was evaluated for anthracnose symptom reduction in applications at 3, 7 or 14 days before the pathogen inoculation, and for its ability of increasing the peroxidase activity. Nineteen isolates and Trichodermil promoted cucumber growth up to 100% and conferred plant protection against anthracnose up to 88.39%. The isolate IB 31/06 reduced the disease severity at the evaluated time intervals. No significant peroxidase increase was observed seven days after treatment with IB 31/06, in the plants treated and infected with the pathogen, in comparison to infected plants. Gene sequencing of th...

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Caracterização de isolados de Colletotrichum lagenarium de pepino com base em marcadores isoenzimáticos

Cited by 2 publications

References 8 publications

Isoenzymatic characterization of Colletotrichum kahawae isolates with different levels of aggressiveness

Isoenzymatic characterization of Colletotrichum kahawae isolates with different levels of aggressiveness

Promoção de crescimento e indução de resistência à antracnose por Trichoderma spp. em pepineiro

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