Isoenzymatic characterization of Colletotrichum kahawae isolates with different levels of aggressiveness

Loureiro, Andreia; Guerra‐Guimarães, Leonor; Lidon, Fernando C.; Bertrand, Benoît; Silva, Maria do Céu; Várzea, Vítor

doi:10.1590/s1982-56762011000500003

Cited by 12 publications

(21 citation statements)

References 23 publications

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“…The detached green berries and hypocotyls were inoculated using the techniques described by Loureiro et al . () and Figueiredo et al . (), respectively.…”

Section: Methodsmentioning

confidence: 85%

“…Using this system, C. kahawae isolates were assigned into different classes and subclasses and in generally presented a similar classification to those previously attributed in other C. kahawae aggressiveness studies (Loureiro et al ., ; Pires et al ., ; Vieira et al ., ). Nevertheless, a few discrepancies were found for some isolates, such as Cam 1 which was classified as highly aggressive (Loureiro et al ., ), Que 2, Ang 6 and Zim 1 which were classified as low aggressive isolates (Pires et al ., ), and Zim 12 as moderately aggressive (Pires et al ., ). In the present study, these isolates revealed a different profile: Zim 1 and Ang 6 were classified as low‐moderate aggressive isolates, Que 2 and Cam 1 as high‐moderate aggressive isolate and Zim 12 as a high aggressive isolate.…”

Section: Discussionmentioning

confidence: 98%

“…The data collected from inoculated green berries and hypocotyls were used to calculate the index of disease intensity (IDI; Loureiro et al ., ) and disease severity (DS; Pires et al ., ) during the time course of the assays, using the following formulae:

IDI = \sum \frac{No. of hypocotyls or green berries in each class \times Each class ranking}{Total no.}

…”

Section: Methodsmentioning

confidence: 99%

“…Despite this, significant differences in the aggressiveness of different C. kahawae isolates (i.e. number of days until the appearance of first symptoms, number of days until complete necrosis of coffee tissues and index of disease severity) have previously been detected and have been referred to in the literature (Beynon et al ., ; Bella et al ., ; Várzea et al ., ; Loureiro et al ., ; Pires et al ., ; Vieira et al ., ). Beynon et al .…”

Section: Introductionmentioning

confidence: 98%

“…More recently, Loureiro et al . () showed that among six isoenzymatic systems, alkaline phosphatase was able to discriminate the most and least aggressive isolates of C. kahawae . Moreover, Pires et al .…”

Section: Introductionmentioning

confidence: 99%

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Aggressiveness profiling of the coffee pathogen Colletotrichum kahawae

et al. 2018

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Colletotrichum kahawae is a specialized plant pathogen of arabica coffee in Africa, able to infect green berries. The economic impact of this pathogen means there is an urgent need to better understand its pathogenic lifestyle, in particular its aggressiveness. In this study, several quantitative traits including disease severity, latent period and incubation period were measured to concomitantly assess the aggressiveness of 26 C. kahawae isolates. The results show that the area under disease progression curve is the most informative variable, particularly when joined together with the index disease intensity 10 days after inoculation and latency period, while the incubation period is not a reliable trait to distinguish aggressiveness levels in C. kahawae. This study also confirms the suitability of hypocotyls and detached green berries to perform C. kahawae aggressiveness assays, revealing that hypocotyls are a more reproducible testing material. Based on isolate profiles, three aggressiveness classes were established (high, moderate and low). A cytological analysis of representative isolates from each class showed that aggressiveness can be related to the development of post‐penetration stages, rather than conidia germination and appressoria differentiation. This study provides, for the first time, the best metrics to evaluate C. kahawae aggressiveness, characterizing the profile of a broad range of isolates, and defining a set of parameters that can be used to classify new isolates. Furthermore, the collected information will contribute to the improvement of coffee breeding programmes, through the selection of tester isolates for prescreening of resistant coffee materials, and offers the opportunity to engage on future genotype–phenotype studies.

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“…The detached green berries and hypocotyls were inoculated using the techniques described by Loureiro et al . () and Figueiredo et al . (), respectively.…”

Section: Methodsmentioning

confidence: 85%