Caracterização de genótipos de cebola com a utilização de marcadores moleculares RAPD

Abstract

RESUMO -Tendo em vista os resultados obtidos, cruzamentos entre os genótipos do primeiro e segundo grupo e destes com o genótipo Crioula Roxa, podem ser melhores por apresentarem maior divergência entre si. A técnica de RAPD mostrou-se eficaz na caracterização molecular dos genótipos de cebola, evidenciando que existe variabilidade entre os genótipos estudados. Palavras-chave: Allium cepa L.. Melhoramento vegetal. Divergência genética.ABSTRACT -The genetic divergence has been measure between fifteen genotypes of onion grown in Santa Catarina using RAPD markers. Eleven primers from Operon Technologies series was used to produced 35 markers, these, 28 were polymorphic. The amplification products were visualized on 1.4% agarose gel stained with ethidium bromide. A similarity matrix using the Jaccard coefficient was constructed with the molecular data. A dendrogram was generated to better visualize the genetic similarity using a clustering method UPGMA. Three groups were formed using the similarity coefficient 0.6 as cutoff point. The first group met genotypes Super Superprecoce and Gauchinha. The second group met twelve genotypes. Into this group, the genotypes Bella Vista and Bella Dura were those with the greatest similarity coefficient, around 0.89. Bela Vista and Superprecoce, Catarina and the hybrid Bella Vista, with similarity coefficient of 0.88 between the pairs. The third group had only the genotype Crioula Roxa, which had the lowest value (0.31) for the similarity coefficient. Given these results, crosses between the genotypes of the first and second group and those with genotype Crioula Roxa can be better, because they have greater divergence between them. The RAPD technique proved effective in the molecular characterization of genotypes of onion, showing that there is variability among the genotypes.

Phenoplasticity of Essential Oils from Two Species of Piper (Piperaceae): Comparing Wild Specimens and Bi-Generational Monoclonal Cultivars

“…The main concerns of the current study are the selection of the explant/matrices, cultivation conditions, medium composition, culture age, genotype, temporal variations, number of subcultures, use of phytohormones, and regeneration methods. These are aspects significant for evaluating the stability and genetic and epigenetic variation of plants regenerated through propagation [76,77]. There are two ways in which epigenetic pro-cesses can contribute to microevolution in natural populations.…”

“…If we find that the descendants of those lineages that have been submitted to different environments remain phenotypically different, and at the same time, they show a significant shift in patterns of DNA methylation, gene, or protein expression, despite being still identical at the DNA level, this is going to be the best evidence for rapid evolution based on epigenetics. This field brings interesting perspectives to studies on natural products [73,77,[80][81][82][83]. To exemplify, associating this notion with human food consumption, a study evaluated the process of domestication of wheat, from three subspecies of Triticum turgidum L. (wild emmer, emmer, and durum wheat).…”

Translation and brazilian cultural adaptation of the Assessment of Life Habits for Children

“…Os elementos de uma matriz de similaridade medem as semelhanças entre pares e quanto maior a similaridade entre dois objetos, maior o valor da medida. Os valores de similaridade observados na matriz podem ser divididos em três grupos e 0,6 pode ser utilizado como ponto de corte 15,16 : mais similares de 0,81 a 1,0; intermediários de 0,61 a 0,80; menos similares: de 0 a 0,60…”

Comparative proteomic expression profiling associations with resistance to onion-Fusarium basal rot

“…Onion (Allium cepa) (Amaryllidaceae) is an important and valuable vegetable crop economically, covers 23.8% of the total vegetable crop production in the globe (Nasr Esfahani et al, 2011;Wamser et al, 2014;Gasser et al, 2023). Onion also contains antioxidant properties, anti-cancer, anti-in ammatory and anti-cholesterol (Khandagale et al, 2022;Sogoba et al, 2022).…”