Características seminais de galos alimentados com rações suplementadas com diferentes óleos e níveis de vitamina E

Abstract

RESUMORealizou-se este trabalho no setor de avicultura de DZO-UFLA, durante o período de junho a outubro de 2003. Foram utilizados 112 galos reprodutores da linhagem Lohmann-LSL, de 16 até 32 semanas de idade. As aves foram distribuídas num delineamento em blocos ao acaso, em arranjo fatorial 3x2 + 1, com três tipos de óleo (Soja, Canola e Girassol), e dois níveis de vitamina E (200 e 400 mg/kg de ração) e o controle (sem óleo e 0 mg de suplementação de vitamina E). As rações experimentais foram elaboradas à base de milho e farelo de soja. Nas 28ª e 29ª semanas foram realizadas coletas de sêmen para avaliação da motilidade, volume do sêmen, concentração, número de células totais e a morfologia espermática. As análises foram realizadas com utilização do programa SAEG. Foi observada interação significativa (P=0,08) entre as fontes de óleos testadas e os níveis de vitamina E, para a motilidade espermática na 28ª semana de idade. Não foi observada diferença significativa (P>0,05) entre as fontes de óleo nem entre os níveis de vitamina E para nenhuma das variáveis estudadas. Conclui-se que qualquer fonte de óleo das utilizadas neste estudo pode ser adicionada à ração de galos reprodutores sem necessidade de elevados níveis de vitamina E.Termos para Indexação: Galos reprodutores, ácidos graxos essenciais, vitamina E. ABSTRACTThe objective of this study was evaluate breeding and productive performance of Lohmann-LSL roosters. A total of 112 breeding roosters from 16 to 32 weeks of age were allotted to a randomized block design in a 3 x 2 + 1 factorial arrangement, with three sources of oil (soybean, canola and sunflower in 3%of inclusion ) and two levels of antioxidant (200 and 400 mg of vitamin E/kg of diet) and the control diet (without oil and 0 mg of supplementation of vitamin E). The experimental diets were made based on corn and soybean meal. At 28 and 29 weeks, semen collection was performed for evaluation of sperm motility, semen volume, concentration and number of total cells. At 29 weeks of age, semen collection was performed to evaluate sperm morphology, rate of osmotic resistance and heat-resistance of spermatozoa. Four roosters per treatment were slaughtered at 30 weeks of age for removal of the testes which were weighted and a sample was obtained for evaluation of the thickness of the seminiferous epithelium, diameter and the population of seminiferous tubule cells. Blood samplings were collected for evaluation of triglycerides and cholesterol. During the experimental period from 16 to 30 weeks , feed intake and total weight gain were evaluated. The true fertility test was performed at 32 weeks of age. The data were evaluated by analysis of variance using the SAEG program. Significant interaction (P<0.05) was found between sources of oil and the levels of vitamin E as well as among the sources of oils for total weight gain, seminiferous epithelium thickness and body weight at 30 weeks of age. Significant difference (P<0.05) was verified among the levels of vitamin E for the levels of triglycerides, choles...

Effect of dietary supplementation with omega-3 and -6 on fresh and frozen/thawed sperm quality of dogs

“…The effects observed in this study in dogs also agree with results observed in other species, including boars supplemented with shark oil (MITRE et al, 2004), sheep with fish oil (ESMAEILI et al, 2012) and roosters with oils containing a high concentration of polyunsaturated fatty acids in the diet, particularly when combined with vitamin E (RODENAS et al, 2005). For stallions, in addition to vitamin E, supplementation with L-carnitine and selenium in combination with omega-3 and -6 resulted in increased motility and integrity of the acrosome and plasma membranes of fresh and frozen/thawed semen (FREITAS et al 2016).…”

“…However, their use as a food supplement to increase semen quality or to treat testicular degeneration has not been fully elucidated in dogs, although it has been reported in other species (ANDREAZZI et al, 2004;MITRE et al, 2004;RODENAS et al, 2005;ATTAMAN et al, 2012;ESMAEILI et al, 2012; SCHMID-LAUSIGK; AURICH, 2014).…”

“…Ingestion of diets containing polyunsaturated fatty acids results in increased fertility due to improved sperm parameters (motility, vigor, concentration, and seminal volume), as described for horses supplemented with flaxseed oil (SCHMID-LAUSIGK; AURICH, 2014), sheep with fish oil (ESMAEILI et al, 2012), pigs with shark oil (MITRE et al, 2004), rabbits with canola oil (ANDREAZZI et al, 2004) and roosters with canola, soybean or sunflower oil with vitamin E (RODENAS et al, 2005).…”

Análise computacional da compactação da cromatina de espermatozoides de galo

“…As demais amostras foram coletadas de aves pertencentes a um lote com 60 semanas de idade, peso médio de 5,3kg e taxa de fertilidade de 96,4 % (galos velhos). Para conservação das amostras, foi colocada uma gota de sêmen em 2ml de formol citrato (Rodenas et al, 2005 …”

Avaliação da suplementação antioxidante na dieta para galos de matrizes pesadas em idade avançada sobre parâmetros reprodutivos

“…Não foi observado efeito significativo sobre o volume de sêmen, o vigor, a motilidade progressiva e a concentração espermática (P>0,05), demonstrando que os antioxidantes dietéticos avaliados não exerceram efeito sobre essas características. Esses resultados estão de acordo com Rodenas et al (2005), os quais não observaram efeito da utilização de antioxidante (vitamina E) na dieta sobre as características seminais de galos de matrizes leves.…”