Características morfofisiológicas de abacaxizeiro 'gomo de mel' enraizado in vitro sob luz natural e substrato vermiculita

Braga, Francyane Tavares; Pasqual, Moacir; Castro, Evaristo Mauro de; Rafael, Gabriel Coimbra

doi:10.1590/s0100-29452011000200027

Cited by 7 publications

(8 citation statements)

References 10 publications

(7 reference statements)

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“…According to Braga et al (2011), increase of mesophyll thickness is plant response to environmental conditions and demonstrates plasticity of adaptation. According to Costa et al (2003), the microporosity of the substrate can reduce gas exchange and water infiltration, and provide water and aeration to the root system, when there is macroporosity in the substrate, thus altering, the characteristics of the mesophyll.…”

Section: Resultsmentioning

confidence: 99%

Initial development of licuri (Syagrus coronata (Mart.) Becc.) –Arecaceae seedlings under different substrates and luminosity levels

2017

Aust. J. Basic & Appl. Sci.

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A R T I C L E I N F O A B S T R A C TLicuri is a palm tree typical of the Brazilian Northeast semi-arid region and of great socioeconomic importance for communities in the region. Despite its importance, few works were developed addressing its propagation and conservation. Studies about luminosity levels and different substrates may result in different growth responses, optimizing the production of seedlings. Therefore, the aim of this study was to evaluate the initial growth and compare the leaf anatomy of licuri seedlings subjected to different substrates and light levels. Licuri seedlings were conditioned in full sun and 50% shading, and the substrates sand, vegetal soil, sand mixed with organic compound and vegetal soil mixed with organic compound. After 90 days of cultivation, was evaluating growth and foliar anatomy. The shoot height, total leaf area, number of leaves, leaf dry weight, stem dry weight and root dry weight showed higher values in the seedlings grown in 50% shading. In full sun, there was an increase only for stem diameter. The dry weight of the root was significantly greater in sand or vegetal soil. For the total leaf area, the same substrates, however, with the presence of the organic compound, presented higher values. Seedlings grown under full sun provided greater thicknesses of adaxial epidermis and adaxial hypodermis. The abaxial epidermis, abaxial hypodermis and mesophyll presented greater thicknesses, regardless of the luminosity tested, in the substrates that contained vegetal soil, with or without the presence of organic compound. The use of sand or vegetable soil and the shaded environment provide better conditions for the production of licuri seedlings. Foliar anatomical analysis is a good indicator to verify the quality of licuri seedlings when grown under different environmental conditions.

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Section: Resultsmentioning

confidence: 99%

Initial development of licuri (Syagrus coronata (Mart.) Becc.) –Arecaceae seedlings under different substrates and luminosity levels

2017

Aust. J. Basic & Appl. Sci.

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“…O aumento na espessura do mesofilo constitui padrão clássico de resposta ao ambiente de cultivo durante a aclimatização (alta intensidade de luz, alta temperatura e baixa umidade relativa do ar em relação à sala de crescimento) e evidencia a plasticidade adaptativa da espécie (Barboza et al, 2006;Braga et al, 2011).…”

Section: Anatomia Foliarunclassified

“…Além da diferença significativa nas espessuras do mesofilo das folhas de abacaxizeiro analisadas, percebeuse, igualmente, diferença no formato das células, demonstrando a sua plasticidade em razão do tratamento utilizado e das épocas de análise (Figura 2). Braga et al (2011) registraram que plantas de abacaxizeiro Gomo de Mel com 60 dias de aclimatização em substrato vermiculita mostraram as maiores espessuras para todos os tecidos do limbo foliar.…”

Section: Comparação Anatômica Entre Plântulas Mantidas In Vitro E Aclunclassified

Morfofisiologia e anatomia foliar de mudas micropropagadas e aclimatizadas de abacaxizeiro cv. Smooth Cayenne em diferentes substratos

et al. 2012

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Morphophysiology and leaf anatomy of plantlets of pineapple cv. Smoth Cayenne acclimatized in different substratesThis study aimed to determine the optimal substrate and ideal time for acclimatization in plants of the 'Smooth Cayenne' pineapple and compare anatomically plantlets cultivated in vitro with acclimatized plants. Shoots were inoculated on MS with BAP (6.66 µM). After three subcultures, the buds were transferred to MS medium for 30 days. The media were solidified with 0.7% agar, and the pH adjusted to 5.8 before autoclaving (120ºC for 20 minutes). Cultures were kept in a growth room (25±1ºC, irradiance of 35 ì mol m-2 s-1 and 16 hours photoperiod). The shoots obtained in vitro were transplanted into plastic pots in a greenhouse, containing the following substrates: T1-Plantmax® + húmus (P + H), T2-Plantmax ® + vermiculite (P + V) and T3-Plantmax ® (P). Anatomical cuts were made on leaves of plants maintained in vitro and plants with 20, 40 and 60 days of acclimatization on different substrates. The use of Plantmax® + húmus substrate provided greater development in the acclimatization of plants. From 40 days of acclimatization, the plants showed anatomical features that may facilitate the adaptation to field conditions.

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“…Alternative substrates have also been used, in order to enhance both root development and the subsequent acclimatization of the plants in greenhouse conditions (ROCHA et al, 2006;VIEIRA et al, 2007;CASANOVA et al, 2008;DAMIANI;SCHUCH, 2009;KAÇAR et al, 2010;AGGARWAL;NIRMALA, 2012;GALDIANO JUNIOR et al, 2012), and might be useful for promoting in vitro rhizogenesis of H. chrysotrichus. In order to achieve better results, liquid medium-soaked vermiculite, perlite, and polyurethane sponges represent promising alternatives to agar and are also less expensive (BRAGA et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, root formation must be considered in the acclimatization and hardening-off periods, which are the last stages of the propagation process, so that the survival and development of micropropagated plants can be improved. It is likely that nutritive media with vermiculite would facilitate the acclimatization process, owing to an increased area of contact between roots and the substrate, which would improve the absorption of water and nutrients (BRAGA et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

AGAR DOES NOT AFFECT IN VITRO RHIZOGENESIS AND EX VITRO ACCLIMATIZATION OF Handroanthus chrysotrichus

Rabaiolli

Reiniger

Stefanel

et al. 2017

CERNE

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ABSTRACT:The golden trumpet tree Handroanthus chrysotrichus (Bignoniaceae) is an important species, owing to its economic, ecological, medicinal, and ornamental value. However, its production is hindered due to lack of seed availability and viability improved methods of vegetative propagation are needed. Therefore, we evaluated the effect of agar on in vitro rhizogenesis of H. chrysotrichus shoots and the subsequent ex vitro acclimatization of the plants obtained. In the in vitro rhizogenesis assay, we cultured the micropropagated shoots on half-strength nutritive Woody Plant Medium (½WPM) supplemented with 30 g . L -1 sucrose, 50 mg . L -1 myoinositol, 30 cm³ of vermiculite, and one of the three agar concentrations (0, 3.5, or 7 g . L -1 ), and after 30 d, we assessed the shoots for root production and leaf number. Thereafter, the plants were subjected to a 49-d ex vitro acclimatization period, which consisted of two stages in a growth room and one stage in a greenhouse; we assessed plant survival, leaf number and increases in stem height at the end of each stage. We found that the addition of agar to the growth medium was not necessary to induce adventitious rooting, especially with respect to secondary roots, and that plants grown on medium without agar had the highest number of leaves after acclimatization. Therefore, agar actually hindered both in vitro rhizogenesis and ex vitro acclimatization. The ex vitro acclimatization result in high survival and resumption of growth and development of H. chrysotrichus micropropagated plants, and this step is not affected by agar on in vitro rhizogenesis. AGAR NÃO AFETA A RIZOGÊNESE IN VITRO E A ACLIMATIZAÇÃO EX VITRO DE Handroanthus chrysotrichus RESUMO:Handroanthus chrysotrichus (Mart. ex DC.) J. Mattos (Bignoniaceae), conhecida como ipê-amarelo, é uma espécie dotada de importância econômica, ecológica e ornamental. No presente trabalho o objetivo foi avaliar o efeito do ágar na rizogênese in vitro e na subsequente aclimatização ex vitro, bem como a eficiência deste processo em relação à sobrevivência e à retomada do crescimento e desenvolvimento das plantas micropropagadas de H. chrysotrichus. Na rizogênese in vitro, brotações micropropagadas na ausência de reguladores de crescimento foram cultivadas in vitro por 30 dias, em meio nutritivo WPM cujas concentrações de sais foram reduzidas à metade (½WPM), acrescido de 30 g . L -1 de sacarose, 50 mg . L -1 de mio-inositol e 30 cm³ de vermiculita, na presença de diferentes concentrações de ágar (0; 3,5 ou 7 g . L -1 ) e ausência de fitorreguladores. Decorrido esse período, as plantas provenientes dos tratamentos do ensaio de rizogênese foram submetidas à aclimatização ex vitro, processo composto por três etapas e duração de 49 dias. O ágar foi dispensável na indução de raízes adventícias, principalmente no que diz respeito às secundárias. Igualmente, na aclimatização, plantas provenientes de cultivo na ausência de ágar apresentaram melhor desempenho, em especial no que diz respeito ao número de folhas. A ausência de ága...

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Características morfofisiológicas de abacaxizeiro 'gomo de mel' enraizado in vitro sob luz natural e substrato vermiculita

Cited by 7 publications

References 10 publications

Initial development of licuri (Syagrus coronata (Mart.) Becc.) –Arecaceae seedlings under different substrates and luminosity levels

Initial development of licuri (Syagrus coronata (Mart.) Becc.) –Arecaceae seedlings under different substrates and luminosity levels

Morfofisiologia e anatomia foliar de mudas micropropagadas e aclimatizadas de abacaxizeiro cv. Smooth Cayenne em diferentes substratos

AGAR DOES NOT AFFECT IN VITRO RHIZOGENESIS AND EX VITRO ACCLIMATIZATION OF Handroanthus chrysotrichus

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