Car cabin filters as sampling devices to study bioaerosols using eDNA and microbiological methods

Hurley, Kirsty V.; Wharton, Laura; Wheeler, M. J.; Skjøth, Carsten Ambelas; Niles, Christian; Hanson, Mary

doi:10.1007/s10453-018-09554-y

Cited by 11 publications

(8 citation statements)

References 49 publications

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“…Filtration is frequently used to collect the bioaerosols [ 36 , 37 ]. The filters are often made of different materials, including polycarbonate, cellulose ester, polytetrafluoroethylene, polyvinyl chloride, nylon, gelatin, glass fiber, alumina nanofiber, and others [ 3 , 17 , 38 ]. According to the physical structure, the filters could be divided into three categories: fibrous filter, porous membrane filter, and capillary pore filter.…”

Section: Bioaerosol Collectionmentioning

confidence: 99%

Challenges and Perspectives for Biosensing of Bioaerosol Containing Pathogenic Microorganisms

Lei

et al. 2021

Micromachines

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As an important route for disease transmission, bioaerosols have received increasing attention. In the past decades, many efforts were made to facilitate the development of bioaerosol monitoring; however, there are still some important challenges in bioaerosol collection and detection. Thus, recent advances in bioaerosol collection (such as sedimentation, filtration, centrifugation, impaction, impingement, and microfluidics) and detection methods (such as culture, molecular biological assay, and immunological assay) were summarized in this review. Besides, the important challenges and perspectives for bioaerosol biosensing were also discussed.

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Section: Bioaerosol Collectionmentioning

confidence: 99%

Challenges and Perspectives for Biosensing of Bioaerosol Containing Pathogenic Microorganisms

Lei

et al. 2021

Micromachines

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“…However, filters become a source of danger to the health of vehicle users if they are not regularly exchanged in a correct way ( 19 , 22 ). Car cabin filters are capable of accumulating contaminants, including pollen, fungi, and microbial fragments, such as proteins ( 23 ).…”

Section: Introductionmentioning

confidence: 99%

Air Disinfection—From Medical Areas to Vehicle

Bukłaha

Wieczorek

Kruszewska

et al. 2022

Front. Public Health

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Cars with air conditioning systems have become the norm, but these systems can be dangerous for human health as a result of the accumulation of different microorganisms, including pathogenic ones, causing severe allergy or inflammation problems. The novel purpose of this study is 2-fold: on the one hand, to test different disinfection agents on a new area, that is, automobile cabins, and on the other, to compare activity in the gas phase of these agents for disinfection of car air conditioning and cabin surfaces. This study shown that tested disinfectant agents dedicated for decontamination medical areas (agent based on peracetic acid and an agent containing didecyldimethylammonium chloride, 2-phenoxyethanol with cinnamaldehyde) can be successfully used for disinfection car air conditioning and cabin surfaces. Both disinfectants were examined in comparison to a commercial “ready-to-use” spray from a local supermarket dedicated to car air conditioning disinfection. Our research found that very effective agents in this regard were acid stabilized by hydrogen peroxide applied by fumigator, and a combination of didecyldimethylammonium chloride, 2-phenoxyethanol, and cinnamaldehyde applied by atomizer. Tested disinfection procedures of car air conditioning significantly influence the quality of cabin air and surfaces by reducing the amount of microorganisms. The comparison of disinfection properties studied agents in the gas phase reveal statistically significant differences between it effect for disinfection car air conditioning and cabin surfaces. Our research found that very effective agents in this regard were acid stabilized by hydrogen peroxide applied by fumigator, and a combination of didecyldimethylammonium chloride, 2-phenoxyethanol, and cinnamaldehyde applied by atomizer. Tested disinfection procedures of car air conditioning significantly influence the quality of cabin air and surfaces by reducing the amount of microorganisms.

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“…Heating, ventilation, and air conditioning (HVAC) systems in cars, trucks and buses have become standard for decades, although with health implications because of the accumulation and recirculation of microorganisms, including those that cause infectious respiratory diseases (1). Mold (fungi), bacteria, pollen and proteins can deposit and grow on filters, heating and cooling coils, and surfaces in the HVAC system (2). These microbes can cause respiratory infectious diseases, allergic reactions and cancer (3).…”

Section: Introductionmentioning

confidence: 99%

Towards a Healthy Car: UVC LEDs Inside Automobile HVAC Chamber Offers Effective Complementary Disinfection to Ensure Clean Cabin Air

Mariita¹,

Davis²,

Lottridge³

et al. 2022

Preprint

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Vehicle HVAC systems can accumulate and recirculate highly infectious respiratory diseases via aerosols. Integrating UVC to complement automobile HVAC systems can protect occupants from developing allergies, experiencing inflammatory problems, or acquiring respiratory infectious diseases by inactivating pathogenic organisms. UVC can add little to no static pressure with minimal space, unlike mercury lamps which are larger and heavier. Additionally, UVC LEDs are effective at low voltage and have no mercury or glass. While previous experiments have shown UVC LED technology can reduce bacteriophage Phi6 concentrations by 1 log in 5 minutes (selected as the average time to clean the cabin air), those studies had not positioned LED within the HVAC itself or studied the susceptibility of the surrogate at the specific wavelength. This study aimed to assess the disinfection performance of UVC LEDs in automotive HVAC systems and determine the dose-response curve for bacteriophage Phi6, a SARS-CoV-2 surrogate. To achieve this, UVC LEDs were installed in a car HVAC system. To determine inactivation efficacy, a model chamber of 3.5 m3, replicating the typical volume of a car, containing the modified automobile HVAC system was filled with bacteriophage Phi6, and the HVAC was turned on with and without the UVC LEDs being turned on. The results revealed that HVAC complemented with UVC reduced bacteriophage Phi6 levels significantly more than the HVAC alone and reduced the viral concentration in the cabin by more than 1 LRV (90% viral reduction) in less than 5 minutes. The performance after 5 minutes is expected to be significantly better against SARS-CoV-2 because of its higher sensitivity to UVC, especially at lower wavelengths (below 270 nm). HVAC alone could not achieve a 90% viral reduction of bacteriophage Phi6 in 15 minutes. Applying UVC LEDs inside an HVAC system is an effective means of quickly reducing the number of aerosolized viral particles in the chamber, by inactivating microorganisms leading to improved cabin air quality.

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Car cabin filters as sampling devices to study bioaerosols using eDNA and microbiological methods

Cited by 11 publications

References 49 publications

Challenges and Perspectives for Biosensing of Bioaerosol Containing Pathogenic Microorganisms

Challenges and Perspectives for Biosensing of Bioaerosol Containing Pathogenic Microorganisms

Air Disinfection—From Medical Areas to Vehicle

Towards a Healthy Car: UVC LEDs Inside Automobile HVAC Chamber Offers Effective Complementary Disinfection to Ensure Clean Cabin Air

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