The aim of this study is to determine the teacher candidates' teaching motivation. In accordance with the nature of the study, the research was carried out according to the mixed method in which quantitative and qualitative data tools were used together. The research was carried out in an explanatory sequential design as a way of mixed methods. While the quantitative method of the research was designed according to the survey model, the phenomenology design was used in the qualitative method. While the quantitative dimension of the study was carried out with 329 pre-service teachers studying at the Education Faculty of a university in Turkey, the qualitative dimension was carried out with 15 preservice teachers who were determined with the maximum sampling of diversity from this sample. The Teaching Motivation Scale, developed by Kauffman, Yılmaz-Soylu, and Duke (2011), adapted to Turkish by Güzel-Candan and Evin-Gencel (2015), was used to collect the quantitative data of the study. In addition, the personal information form developed by the researcher was used to collect the demographic characteristics of the participants in the study. In the qualitative part of the study, a semistructured interview form was used to determine the factors affecting teacher candidates' teaching motivation. The quantitative findings of the study showed that teacher candidates' teaching motivation was at a high level. İn addition, it was determined that teacher candidates' teaching motivations differed significantly according to their gender, the programs they studied, and the class level. The qualitative results of the study showed that the positive factors affecting the teaching motivation of the teacher candidates were more than the negative ones. In this regard, it can be said that the quantitative and qualitative findings overlap. Based on the findings of the study, some recommendations for future research were presented.