Abstract:In order to identify factors involved in transcription of human snRNA genes and 3′ end processing of the transcripts, we have carried out CRISPR affinity purification in situ of regulatory elements (CAPTURE), which is deadCas9-mediated pull-down, of the tandemly repeated U2 snRNA genes in human cells. CAPTURE enriched many factors expected to be associated with these human snRNA genes including RNA polymerase II (pol II), Cyclin-Dependent Kinase 7 (CDK7), Negative Elongation Factor (NELF), Suppressor of Ty 5 (… Show more
“…At present, CAPTURE has played a huge role in the regulation mechanism of the snRNA gene, mouse embryonic stem cells, and other fields. 61 , 62 It can also provide new paths to discover novel drug targets.…”
Section: Gene Editing Technology Based On Crispr/cas9 Systemmentioning
“…At present, CAPTURE has played a huge role in the regulation mechanism of the snRNA gene, mouse embryonic stem cells, and other fields. 61 , 62 It can also provide new paths to discover novel drug targets.…”
Section: Gene Editing Technology Based On Crispr/cas9 Systemmentioning
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