Capture-free deactivation of CTCs in the bloodstream; a metastasis suppression method by electrostatic stimulation of the peripheral blood

Ghaderinia, Mohammadreza; Khayamian, Mohammad Ali; Abadijoo, Hamed; Shalileh, Shahriar; Faramarzpour, Mahsa; Zandi, Ashkan; Simaee, Hossein; Abbasvandi, Fereshteh; Esmailinejad, Mohammad Reza; Rafizadeh-Tafti, Saeed; Jahangiri, Mojtaba; Kordehlachin, Yasin; Ghaffari, Hadi; Ansari, Ehsan; Dabbagh, Najmeh; Akbari, Mohammad Esmaeil; Hoseinpour, Parisa; Abdolahad, Mohammad

doi:10.1016/j.bios.2021.113194

Cited by 11 publications

(8 citation statements)

References 39 publications

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“…In line with our hypothesis, the role of intrinsic electrostatic charge in one type of phospholipid membrane, which tunes the KRAS and MAPK mitotic pathways in malignant cells, was also revealed in other studies ( 7 ). Another research showed that the pure positive electrostatic stimulation induces the deterioration of circulating cancer cells in the blood, while it has no impact on normal cells ( 4 ). It assumed that a high-intensity electrostatic field changes the ionic flow and depolarizes the cell surface charge, resulting in an ionic imbalance in invasive, proliferative, and tumorigenic cancer cells and deterioration of their ionic channels ( 2 , 3 ).…”

Section: Resultsmentioning

confidence: 99%

“…Previous studies reported the role of positive electrostatic charge treating (PECT) in the selective suppressing effect of the proliferation and invasion of malignant tumors with an efficacy of more than 90% on cancerous cell lines and mouse models (1)(2)(3)(4). The advantage of PECT in comparison with other electrical field-based cancer therapies, such as electrochemical treatment or irreversible electroporation, is that there is no requirement for current flow into the body (which might be dangerous if it becomes noncontrollable (5,6)).…”

Section: Introductionmentioning

confidence: 99%

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A human pilot study on positive electrostatic charge effects in solid tumors of the late-stage metastatic patients

Zandi,

Shojaeian,

Abbasvandi

et al. 2023

Front. Med.

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BackgroundCorrelative interactions between electrical charges and cancer cells involve important unknown factors in cancer diagnosis and treatment. We previously reported the intrinsic suppressive effects of pure positive electrostatic charges (PEC) on the proliferation and metabolism of invasive cancer cells without any effect on normal cells in cell lines and animal models. The proposed mechanism was the suppression of pro-caspases 3 and 9 with an increase in Bax/Bcl2 ratio in exposed malignant cells and perturbation induced in the KRAS pathway of malignant cells by electrostatic charges due to the phosphate molecule electrostatic charge as the trigger of the pathway. This study aimed to examine PECs as a complementary treatment for patients with different types of solid metastatic tumors, who showed resistance to chemotherapy and radiotherapy.MethodsIn this study, solid metastatic tumors of the end-stage patients (n = 41) with various types of cancers were locally exposed to PEC for at least one course of 12 days. The patient’s signs and symptoms, the changes in their tumor size, and serum markers were followed up from 30 days before positive electrostatic charge treating (PECT) until 6 months after the study.ResultsEntirely, 36 patients completed the related follow-ups. Significant reduction in tumor sizes and cancer-associated enzymes as well as improvement in cancer-related signs and symptoms and patients’ lifestyles, without any side effects on other tissues or metabolisms of the body, were observed in more than 80% of the candidates.ConclusionPECT induced significant cancer remission in combination with other therapies. Therefore, this non-ionizing radiation would be a beneficial complementary therapy, with no observable side effects of ionizing radiotherapy, such as post-radiation inflammation.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A human pilot study on positive electrostatic charge effects in solid tumors of the late-stage metastatic patients

Zandi,

Shojaeian,

Abbasvandi

et al. 2023

Front. Med.

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“…In harmony with the AO/PI assay, the results of the Annexin V/PI test also show no apoptosis in the non-activated immune cells ( Figure 3F ) after 48 h of AEF treatment. The trajectory of the WBCs ( Figures 3G,H ) as well as their displacement and velocity ( Ghaderinia et al, 2021 ) were also analyzed for non-activated WBCs in the presence and absence of AEF. As shown in Figures 3I,J , no difference could be inferred in their natural moving behavior.…”

Section: Resultsmentioning

confidence: 99%

Healing Field: Using Alternating Electric Fields to Prevent Cytokine Storm by Suppressing Clonal Expansion of the Activated Lymphocytes in the Blood Sample of the COVID-19 Patients

Abadijoo

Khayamian

Faramarzpour

et al. 2022

Front. Bioeng. Biotechnol.

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In the case of the COVID-19 early diagnosis, numerous tech innovations have been introduced, and many are currently employed worldwide. But, all of the medical procedures for the treatment of this disease, up to now, are just limited to chemical drugs. All of the scientists believe that the major challenge toward the mortality of the COVID-19 patients is the out-of-control immune system activation and the subsequent cytokine production. During this process, the adaptive immune system is highly activated, and many of the lymphocytes start to clonally expand; hence many cytokines are also released. So, any attempt to harness this cytokine storm and calm down the immune outrage is appreciated. While the battleground for the immune hyperactivation is the lung ambient of the infected patients, the only medical treatment for suppressing the hypercytokinemia is based on the immunosuppressor drugs that systemically dampen the immunity with many unavoidable side effects. Here, we applied the alternating electric field to suppress the expansion of the highly activated lymphocytes, and by reducing the number of the renewed cells, the produced cytokines were also decreased. Applying this method to the blood of the COVID-19 patients in vitro showed ∼33% reduction in the average concentration of the three main cytokines after 4 days of stimulation. This method could carefully be utilized to locally suppress the hyperactivated immune cells in the lung of the COVID-19 patients without any need for systemic suppression of the immune system by the chemical drugs.

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“…Curcumin loaded poly(lactic-co-glycolic acid) nanoparticles and laser irradiation (λ = 447 nm, P = 100 mW) result in photodynamic inactivation of CTCs [152]. Electrostatic charge stimulation (PPECS) of the whole blood selectively deactivates the invasive function of CTCs and suppresses pulmonary metastasis of 4T1 breast cancer cells and extended survival in an in vivo model [153]. Combining bionic NSK (nanosponges and nanokillers-platelet-neutrophil hybrid membrane (PNM)-camouflaging gold nanocages (AuNCs)) with chemo-photothermal therapy is effective for actively clearing CTCs, neutralizing migrating tumor-derived exosomes, activating the innate immune system, and inhibiting breast cancer metastasis in 4T1 xenograft and orthotopic breast tumor-bearing mice [154].…”

Section: Other Methods For Ctc Eradicationmentioning

confidence: 99%

Metastasis Prevention: Focus on Metastatic Circulating Tumor Cells

et al. 2021

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Metastasis is the main cause of cancer death. Metastatic foci are derived from tumor cells that detach from the primary tumor and then enter the circulation. Circulating tumor cells (CTCs) are generally associated with a high probability of distant metastasis and a negative prognosis. Most CTCs die in the bloodstream, and only a few cells form metastases. Such metastatic CTCs have a stem-like and hybrid epithelial-mesenchymal phenotype, can avoid immune surveillance, and show increased therapy resistance. Targeting metastatic CTCs and their progenitors in primary tumors and their descendants, particularly disseminated tumor cells, represents an attractive strategy for metastasis prevention. However, current therapeutic strategies mainly target the primary tumor and only indirectly affect metastasis-initiating cells. Here, we consider potential methods for preventing metastasis based on targeting molecular and cellular features of metastatic CTCs, including CTC clusters. Also, we emphasize current knowledge gaps in CTC biology that should be addressed to develop highly effective therapeutics and strategies for metastasis suppression.

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Capture-free deactivation of CTCs in the bloodstream; a metastasis suppression method by electrostatic stimulation of the peripheral blood

Cited by 11 publications

References 39 publications

A human pilot study on positive electrostatic charge effects in solid tumors of the late-stage metastatic patients

A human pilot study on positive electrostatic charge effects in solid tumors of the late-stage metastatic patients

Healing Field: Using Alternating Electric Fields to Prevent Cytokine Storm by Suppressing Clonal Expansion of the Activated Lymphocytes in the Blood Sample of the COVID-19 Patients

Metastasis Prevention: Focus on Metastatic Circulating Tumor Cells

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