Capsid-Targeted Viral Inactivation: A Novel Tactic for Inhibiting Replication in Viral Infections

Zhang, Xingcui; Jia, Renyong; Zhou, Jiakun; Wang, Mingshu; Yin, Zhongqiong; Cheng, Anchun

doi:10.3390/v8090258

Cited by 8 publications

(10 citation statements)

References 101 publications

(108 reference statements)

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“…Based on the combination of C protein and RNA, researchers have considered fusing exogenous genes with C protein through a capsid-targeted antiviral inactivation (CTVI) strategy [ 62 ]. This tactic is based on the fusion of the C protein and a crucial effector molecule, such as a nuclease, a lipase, a protease, or a single-chain antibody (scAb), which can degrade viral DNA/RNA or interfere with the proper folding of important viral proteins [ 63 ].…”

Section: Multiple Functions Of the C Proteinmentioning

confidence: 99%

Structure and function of capsid protein in flavivirus infection and its applications in the development of vaccines and therapeutics

Zhang

Jia

et al. 2021

Vet Res

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Flaviviruses are enveloped single positive-stranded RNA viruses. The capsid (C), a structural protein of flavivirus, is dimeric and alpha-helical, with several special structural and functional features. The functions of the C protein go far beyond a structural role in virions. It is not only responsible for encapsidation to protect the viral RNA but also able to interact with various host proteins to promote virus proliferation. Therefore, the C protein plays an important role in infected host cells and the viral life cycle. Flaviviruses have been shown to affect the health of humans and animals. Thus, there is an urgent need to effectively control flavivirus infections. The structure of the flavivirus virion has been determined, but there is relatively little information about the function of the C protein. Hence, a greater understanding of the role of the C protein in viral infections will help to discover novel antiviral strategies and provide a promising starting point for the further development of flavivirus vaccines or therapeutics.

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Section: Multiple Functions Of the C Proteinmentioning

confidence: 99%

Structure and function of capsid protein in flavivirus infection and its applications in the development of vaccines and therapeutics

Zhang

Jia

et al. 2021

Vet Res

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“…Moreover, the result of direct inactivation of the virus particle demonstrated that the non-sericin extract had the most virucidal effect against both HSV-1 and HSV-2 in a dose and time-dependent manner ( Figure 2 ). We hypothesized that bioactive compounds in non-sericin extract might contribute to the inhibition through deconstructing the virion envelope structure or inducing the glycoprotein conformational change [ 32 , 33 , 34 ]. However, further investigation is needed to demonstrate the effect of extracts on viral structure i.e., cryo-EM to reveal changes of the virus surface.…”

Section: Discussionmentioning

confidence: 99%

Anti-Herpes Simplex Virus Efficacy of Silk Cocoon, Silkworm Pupa and Non-Sericin Extracts

et al. 2021

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Herpes simplex virus (HSV) infections are prevalent worldwide and are the cause of life- threatening diseases. Standard treatment with antiviral drugs, such as acyclovir, could prevent serious complications; however, resistance has been reported specifically among immunocompromised patients. Therefore, the development of an alternative approach is needed. The silk cocoon derived from silkworm, Bombyx mori, has been recognized for its broad-spectrum biological activity, including antiviral activity; however, its effects against HSV infection are unknown. In this study, we investigated the inhibitory effects of silk extracts derived from the cocoon shell, silk cocoon, silkworm pupa and non-sericin extract, on blocking HSV-1 and HSV-2 binding to host cells, resulting in the inhibition of the virus infection in Vero cells. Non-sericin extract demonstrated the greatest effectiveness on inhibiting HSV-1 and HSV-2 binding activity. Moreover, the virucidal effect to inactivate HSV-1 and HSV-2 was determined and revealed that non-sericin extract also exerted the highest potential activity. Using the treatment of non-sericin extract in HSV-2-infected HeLa cells could significantly lower the HSV-induced cell death and prevent inflammation via lowering the inflammatory cytokine gene expression. The non-sericin extract was analyzed for its bioactive compounds in which gallic acid, flavonoid and xanthophyll were identified, and might have partially contributed to its antiviral activity. The finding in our study suggested the potential of silk extract as an alternative therapeutic treatment for HSV infection.

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“…423–439]. The virus capsid assembly and DNA packaging have been proposed to be the target for antiviral therapy [ 34 ]. Thus, biofilm formation may share a similar group of involved genes with viral procapsid maturation and biofilm formation may be suggested as the target for antimicrobial treatment.…”

Section: Discussionmentioning

confidence: 99%

Mechanism Underlying the Role of LuxR Family Transcriptional Regulator abaR in Biofilm Formation by Acinetobacter baumannii

Sun

Xiang

2021

Curr Microbiol

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Our study attempted to explore the mechanism underlying the role of LuxR family transcriptional regulator abaR in biofilm formation by Acinetobacter baumannii. The abaR gene was knocked out in ATCC 17978 strain using homologous recombination method. The growth curve and biofilm formation were measured in the wild type and abaR gene knockdown strains. Transcriptome sequencing was performed in the wild type and abaR gene knockdown strains following 8 h of culture. The growth curve in the abaR gene knockdown strain was similar to that of the wild-type strain. Biofilm formation significantly declined in the abaR gene knockdown strain at 8 and 48 h after culture. A total of 137 differentially expressed genes (DEGs) were obtained including 20 downregulated DEGs and 117 upregulated DEGs. Genes with differential expression were closely related to viral procapsid maturation (GO:0046797), acetoin catabolism (GO:0045150), carbon metabolism (ko01200), and the glycolysis/gluconeogenesis (ko00010)-related pathways. The results of the eight verified expression DEGs were consistent with the results predicted by bioinformatics. AbaR gene knockdown significantly affected biofilm formation by A. baumannii ATCC 17978 strain. The glycolysis/gluconeogenesis pathways were significantly dysregulated and induced by abaR gene knockdown in A. baumannii. Supplementary Information The online version contains supplementary material available at 10.1007/s00284-021-02654-y.

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Capsid-Targeted Viral Inactivation: A Novel Tactic for Inhibiting Replication in Viral Infections

Cited by 8 publications

References 101 publications

Structure and function of capsid protein in flavivirus infection and its applications in the development of vaccines and therapeutics

Structure and function of capsid protein in flavivirus infection and its applications in the development of vaccines and therapeutics

Anti-Herpes Simplex Virus Efficacy of Silk Cocoon, Silkworm Pupa and Non-Sericin Extracts

Mechanism Underlying the Role of LuxR Family Transcriptional Regulator abaR in Biofilm Formation by Acinetobacter baumannii

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