Capilliposide from Lysimachia capillipes inhibits AKT activation and restores gefitinib sensitivity in human non-small cell lung cancer cells with acquired gefitinib resistance

Zhang, Shirong; Yan, Xu; Jin, Er; Zhu, Lucheng; Xia, Bing; Chen, Xufeng; Li, Fanzhu; Ma, Shenglin

doi:10.1038/aps.2016.116

Cited by 15 publications

(8 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We next evaluated the multiplex ORNi-PCR method with gDNA extracted from four other lung cell lines, RERF-LC-KJ, LC-2/ad, NCI-H1299, and MRC-5, of which only LC-2/ad harbors the EGFR L858R (T2573G) mutation [18,22,23,24]. However, multiplex ORNi-PCR amplified the 0.4 and 0.6 kbp products only from NCI-H1975 gDNA (Figure 3C).…”

Section: Resultsmentioning

confidence: 99%

Simultaneous Detection of the T790M and L858R Mutations in the EGFR Gene by Oligoribonucleotide Interference-PCR

Baba

Fujita

Tasaka

et al. 2019

IJMS

View full text Add to dashboard Cite

A de novo single-nucleotide mutation in the EGFR gene can cause the development of lung cancer. EGFR tyrosine kinase inhibitors (EGFR-TKIs) are used for clinical treatment of such lung cancers, but acquired resistance often mitigates their efficacy. Accordingly, monitoring of de novo and acquired nucleotide mutations is essential for clinical treatment of lung cancers with EGFR-TKIs. Previously, we reported that oligoribonucleotide interference-PCR (ORNi-PCR) can accurately and cost-effectively detect single-nucleotide mutations. In this study, we applied ORNi-PCR to simultaneous detection of the de novo L858R and acquired T790M mutations in the EGFR gene in lung cancer cells. First, we established optimal experimental conditions for ORNi-PCR to simultaneously detect the two single-nucleotide mutations in genomic DNA from lung cancer cells. The conditions we established could also be used for ORNi-PCR using complementary DNA reverse-transcribed from extracted RNA. We found that ORNi-PCR could detect lung cancer cells possessing both single-nucleotide mutations among a large number of cells harboring wild-type sequences, even when the cancer cells constituted less than ~0.2% of all cells. Our findings demonstrate that ORNi-PCR can simultaneously detect multiple single-nucleotide mutations in a gene of interest and might therefore be useful for simultaneous detection of EGFR mutations in clinical examinations.

show abstract

Section: Resultsmentioning

confidence: 99%

Simultaneous Detection of the T790M and L858R Mutations in the EGFR Gene by Oligoribonucleotide Interference-PCR

Baba

Fujita

Tasaka

et al. 2019

IJMS

View full text Add to dashboard Cite

show abstract

“…CAPB and CAPC, two major compounds of L. capillipes Hemsl, exhibit significant cytotoxicity against many human cancer cell lines, including prostate cancer cell PC3 and DU145, along with nasopharyngeal cancer CNE-2 cells; ovarian cancer SK-OV-3 and A2780 subtypes; and lung cancer PC-9, A549, H1299, and H460 cells [25,26,27,28,29,30]. Moreover, CAPB and CAPC were found to inhibit tumor growth without inducing significant toxicity to hepatic or renal tissues in a mouse model [26,27,32].…”

Section: Discussionmentioning

confidence: 99%

“…On one hand, systemic exposure of CAPB and CAPC was low with significant variation among individuals after oral administration. This is likely a result of its extensive biotransformation in the gastrointestinal tract [30]. On the other hand, the low intestinal permeability of CAPB and CAPC may also serve as a critical barrier, resulting in poor bioavailability and low exposure in tissues after oral administration [31].…”

Section: Introductionmentioning

confidence: 99%

In Vitro and In Situ Characterization of the Intestinal Absorption of Capilliposide B and Capilliposide C from Lysimachia capillipes Hemsl

Zhang

Cheng

et al. 2019

Molecules

View full text Add to dashboard Cite

The goal of this investigation was to determine the processes and mechanism of intestinal absorption for capilliposide B (CAPB) and capilliposide C (CAPC) from the Chinese herb, Lysimachia capillipes Hemsl. An analysis of basic parameters, such as drug concentrations, time, and behavior in different intestinal segments was analyzed by liquid chromatography-tandem mass spectrometry (LC-MS). The susceptibility of CAPB and CAPC to various inhibitors such as P-glycoprotein (P-gp) inhibitor (verapamil); multidrug resistance-associated protein 2 (MRP2) inhibitor (indomethacin); cytochrome P450 protein 3A4 (CYP3A4) inhibitor (ketoconazole); and the co-inhibitor of P-gp, MRP2 and CYP3A4 (cyclosporine A) were assessed using both caco-2 cell monolayer and single-pass intestinal perfusion (SPIP) models. As a result, CAPB and CAPC are both poorly absorbed in the intestines and exhibited segment-dependent permeability. The intestinal permeability of CAPB and CAPC were significantly increased by the co-treatment of verapamil, indomethacin. In addition, the intestinal permeability of CAPB was also enhanced by ketoconazole and cyclosporine A. It can be concluded that the intestinal absorption mechanisms of CAPB and CAPC involve processes such as facilitated passive diffusion, efflux transporters, and enzyme-mediated metabolism. Both CAPB and CAPC are suggested to be substrates of P-gp and MRP2. However, CAPB may interact with the CYP3A4 system.

show abstract

“…From the tenth day, intratumoral injection of 50 μL of the extract of V. album (15 mg / kg) was injected every day for 15 days while tumor formation was initiated. During the experiment, the tumor size was measured using a ruler every two days by formula: Tumor size = (length x width 2 )/2 [22]. At the end of the experiment, the data about the size of tumor tissue were evaluated by one-way ANOVA analysis using GraphPad InStat 3.0 statistical software.…”

Section: Methodsmentioning

confidence: 99%

The Effects of Medicinal Plants on Cancer Cell Lines and Efficacy of Experimental Animal Model

Tuğlu¹,

Aydemir²,

Sonmez³

et al. 2017

International Journal of Secondary Metabolite

View full text Add to dashboard Cite

The use of medicinal plants as an alternative treatment is a historical process and has been known for a long time so that classical treatment can be more effective in wound and cancer treatment. The purpose of this study was to investigate the effects of plant extracts used for therapeutic purposes in cancer cell lines in vitro wound model and in vivo experimental animal model in order to obtain this information. The plants, olive oil (Oleocanthal), mistletoe (Viscum album), Common Centaury (Centaurium erythraea), Momordica charantia, Inula viscosa, Citrus aurantium, Thyme oil (Thymus vulgaris) and algae (Jania longifurca), were used. MCF-7, MB-MDA-231, 67NR and 4T1 for breast, NB2a for neuron, L929 for fibroblast and normal somatic mesenchymal stem cell for comparison were selected for in vitro wound models. As an in vivo breast cancer model, female Balb/c mice were injected with 4T1 cells and skin wound healing in rats was investigated. The effects of medicinal plants were evaluated using MTT assay for viability and proliferation, immunocytochemistry staining NOS for oxidative stress and TGFbeta1 for wound healing. It was found that plant extracts reduced antioxidative damage and inhibited apoptosis. It was observed that oxidative stress and apoptosis were increased in cancer cells, but less effective in invasive cell lines. In vivo experiments showed that wound healing was accelerated and that these rates were achieved with antioxidative and antiapoptotic effects. It has been concluded that medicinal plants are beneficial for treatment of difficult diseases in which patient quality of life is very effective and they should be used as scientific-based medical applications.

show abstract

Capilliposide from Lysimachia capillipes inhibits AKT activation and restores gefitinib sensitivity in human non-small cell lung cancer cells with acquired gefitinib resistance

Cited by 15 publications

References 56 publications

Simultaneous Detection of the T790M and L858R Mutations in the EGFR Gene by Oligoribonucleotide Interference-PCR

Simultaneous Detection of the T790M and L858R Mutations in the EGFR Gene by Oligoribonucleotide Interference-PCR

In Vitro and In Situ Characterization of the Intestinal Absorption of Capilliposide B and Capilliposide C from Lysimachia capillipes Hemsl

The Effects of Medicinal Plants on Cancer Cell Lines and Efficacy of Experimental Animal Model

Contact Info

Product

Resources

About