Capillary zone electrophoresis-mass spectrometry using an electrospray ionization interface

Smith, Richard D.; Olivares, José A.; Nguyen, Nhung T.; Udseth, Harold R.

doi:10.1021/ac00156a013

Cited by 386 publications

(200 citation statements)

References 8 publications

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“…The number of analyte ions that are transferred from one region to the next is proportional to the current density (J) at the entrance orifice of the mass spectrometer and the gas flow into the MS at that orifice [13]. Since ESI is typically performed at atmospheric pressure, the efficient transfer of gas phase ions from ambient pressure to high vacuum required for mass spectrometry was recognized as an important sensitivity issue during the technique's infancy [14,15]. Due to the coulombically repulsive nature of an ESI plume and the characteristic losses of skimmer devices, ion transmission from source to detector in ESI-MS has been quoted as being as low as 1 ion transmitted in 10 5 ions created [2,16].…”

mentioning

confidence: 99%

Investigation of electrospray ionization and electrostatic focusing devices using a three-dimensional electrospray current density profiler

Thompson

Eschelbach

Wilburn

et al. 2005

J. Am. Soc. Mass Spectrom.

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A novel instrument for profiling the current density of nanoelectrospray ionization plumes in three dimensions has been developed. A hemispherically-shaped electrostatic lens at atmospheric pressure is found to be able to compress the space-charge in nano-ESI and increase the average current density in the plume to three times the nominal value. Ion transmission into a single-quadrupole mass spectrometer is found to roughly double using the electrostatic lens. Data also suggest that ion transmission into the first vacuum region for a skimmer-type mass spectrometer interface using nano-ESI may be typically 40% or better with no special focusing device used. (J Am Soc Mass Spectrom 2005, 16, 312-323)

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mentioning

confidence: 99%

Investigation of electrospray ionization and electrostatic focusing devices using a three-dimensional electrospray current density profiler

Thompson

Eschelbach

Wilburn

et al. 2005

J. Am. Soc. Mass Spectrom.

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“…The strong response of QAs using ion evaporation is well documented (Smith et al, 1988). Detection limits in the femtogram range have been reported (Conboy et al, 1990).…”

Section: Detection Limits Of P(etap)thmentioning

confidence: 94%

“…(2) The strongly basic QA was included as a group mediating efficient ionization for detecting amino acid derivatives by ion-evaporation MS at a high sensitivity. It has previously been shown that strongly basic groups such as QAs and strongly acidic groups such as sulfates or sulfonates are detectable at low femtomole levels by ion-evaporation MS (Smith et al, 1988;Conboy et al, 1990;Mueck & Henion, 1990 -Liebold, 1989). (4) PETAPITC is polar.…”

Section: Design Of Novel Sequencing Reagentmentioning

confidence: 99%

Design, synthesis, and characterization of a protein sequencing reagent yielding amino acid derivatives with enhanced detectability by mass spectrometry

et al. 1992

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We report the design, chemical synthesis, and structural and functional characterization of a novel reagent for protein sequence analysis by the Edman degradation, yielding amino acid derivatives rapidly detectable at high sensitivity by ion-evaporation mass spectrometry. We demonstrate that the reagent 3-[4'(ethylene-N, N, N-trimethylamino)phenyl]-2-isothiocyanate is chemically stable and shows coupling and cyclization/cleavage yields comparable to phenylisothiocyanate, the standard reagent in chemical sequence analysis, under conditions typically encountered in manud or automated sequence analysis. Amino acid derivatives generated with this reagent were detectable by ion-evaporation mass spectrometry at the subfemtomole sensitivity level at a pace of one sample per minute. Furthermore, derivatives were identified by their mass, thus permitting the rapid and highly sensitive determination of the molecular nature of modified amino acids. Derivatives of amino acids with acidic, basic, polar, or hydrophobic side chains were reproducibly detectable at comparable sensitivities. The polar nature of the reagent required covalent immobilization of polypeptides prior to automated sequence analysis. This reagent, used in automated sequence analysis, has the potential for overcoming the limitations in sensitivity, speed, and the ability to characterize modified amino acid residues inherent in the chemical sequencing methods that are currently used.

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“…Thus, it is necessary to create stable electrical and physicochemical conditions for the successful formation of ions, and hence, for successful analysis. [18] …”

Section: Introductionmentioning

confidence: 99%

Homemade Capillary Electrophoresis Coupled to a Mass Spectrometer

Moraes

Carrilho

Assunção

2014

Journal of Liquid Chromatography & Related Technologies

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A system was developed in our laboratory to couple capillary electrophoresis with mass spectrometry. The capillary electrophoresis system was equipped with a high voltage supply, and a microcontroller with assembly language programming was developed for the computational control of the system. The MS system was a commercial Thermo Finningan LCQ ion trap mass spectrometer. The robustness of the coupled system was evaluated using standard protein samples (lysozyme, aprotinin, and bovine albumin) and tryptic digests of lysozyme. The system showed positive results in terms of robustness, allowing for the separation of digested proteins and the identification of 33% of the total amino acids in a protein (6 of the 18 expected peptides). The limit of detection was in the order of 1 picomole (signal-to-noise ratio), which was considered satisfactory for this system. The system shows high versatility in tandem coupling and combinations with other analytical procedures.

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Capillary zone electrophoresis-mass spectrometry using an electrospray ionization interface

Cited by 386 publications

References 8 publications

Investigation of electrospray ionization and electrostatic focusing devices using a three-dimensional electrospray current density profiler

Investigation of electrospray ionization and electrostatic focusing devices using a three-dimensional electrospray current density profiler

Design, synthesis, and characterization of a protein sequencing reagent yielding amino acid derivatives with enhanced detectability by mass spectrometry

Homemade Capillary Electrophoresis Coupled to a Mass Spectrometer

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