Capillary zone electrophoresis in wide bore capillary tubes with fiber-coupled diode array detection

Strašı́k, Stanislav; Danková, Mariana; Molnárová, Markéta; Ölvecká, Eva; Kaniansky, Dušan

doi:10.1016/s0021-9673(02)01813-7

Cited by 19 publications

(13 citation statements)

References 47 publications

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“…In this context, we should note that the ITP-CZE-DAD combination (see ref. [32,33]) very likely might be applicable for our enantioseparations [44], especially, while enhancing the CE-DAD identification power in a general way, as suggested previously [32].…”

Section: Introductionmentioning

confidence: 66%

“…2a. We followed the recommendation published in the literature [32] to minimize the impact of the electrolyte system on the analyte spectrum. An average spectrum calculated from the spectra acquired before the start and behind the end of the peak of each PHM enantiomer in the actual ITP-CZE run was used for the background correction.…”

Section: Proof Of Migration Selectivity By Dad Spectramentioning

confidence: 99%

“…A parameter which is independent of the measurement units used is obtained if the covariance is divided by the SD of both sets of measurements. This quantity is the product-moment correlation coefficient or the Pearson correlation coefficient [50] which served as a numerical criterion (matching factor) expressing a match of the analyte spectrum with the reference spectrum [32]. Spectra of PHM enantiomers acquired by the DAD in the CZE stage of separation under the working conditions identical with those used in the ITP-CZE analysis of urine samples, served as the reference spectra in the confirmation of identity of those substances in model and urine samples.…”

Section: Applicationmentioning

confidence: 99%

“…CZE-DAD, for example, contributes to identification and determination of biologically active compounds like drugs and their structural analogs and metabolites, in body fluids. The use of fiber-coupled DAD devices to CZE benefit from wider bore capillaries, as discussed in previous papers [32,33]. This approach is analytically very advantageous, especially, when based on the ITP-CZE-DAD combination as introduced previously [12].…”

Section: Introductionmentioning

confidence: 97%

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Enantioselective analysis of pheniramine in urine by charged CD‐mediated CZE provided with a fiber‐based DAD and an on‐line sample pretreatment by capillary ITP

et al. 2007

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Application potentialities of CZE on-line coupled with capillary ITP and DAD to the identification and determination of trace concentration levels (microg/L) of pheniramine (PHM) enantiomers and their metabolites present in complex ionic matrices of biological origin (urine) are shown. An enhanced (enantio)selectivity of the CZE separation system obtained by the addition of carboxyethyl-beta-CD (CE-beta-CD) to the carrier electrolyte provided CZE conditions for a reliable identification of similar/identical DAD spectra of structurally related compounds (PHM enantiomers and their metabolites) in clinical urine samples differing in qualitative and quantitative composition of sample matrix constituents. A high sample loadability (a 30 microL sample injection volume), partial sample clean-up (removing macroconstituents from the sample), and preconcentration of the analytes in ITP stage resulted in the decrease of concentration LOD for PHM enantiomers in urine to 5.2 and 6.8 microg/L (2.2 x 10(-8) and 2.8 x 10(-8) mol/L), without using any sample pretreatment technique. The background correction and smoothing procedure applied to the raw DAD spectra provided analytically relevant DAD spectra of PHM enantiomers and their metabolites also when they were present in urine sample (30 microL injection volumes of ten-times diluted urine sample) at a 9 x 10(-) (8) mol/L concentration. DAD spectra of PHM enantiomers present in urine samples matched their reference spectra with reasonable certainties. DAD spectra of PHM metabolites were compared with the reference spectra of PHM enantiomers and a good match was found which indicates the similarities in the structures of enantiomers and their metabolites detected in the urine samples. This fact allows performing the quantitative analyses of PHM metabolites in the urine samples by applying the calibration parameters of PHM enantiomers also for PHM metabolites and the results show the possibilities of using the ITP-CZE-DAD combination for the direct analysis of PHM enantiomers and/or their metabolites in urine without any sample pretreatment. ITP-CZE-DAD method with oppositely charged selector is suggested to use in clinical research as it provides favorable performance parameters including sensitivity, linearity, precision, recovery, and robustness with minimal demands on sample preparation.

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Section: Introductionmentioning

confidence: 66%

Section: Proof Of Migration Selectivity By Dad Spectramentioning

confidence: 99%

Section: Applicationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

See 2 more Smart Citations

Enantioselective analysis of pheniramine in urine by charged CD‐mediated CZE provided with a fiber‐based DAD and an on‐line sample pretreatment by capillary ITP

et al. 2007

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“…Homogeneity of QUI spectra was expressed via Pearson's correlation coefficients (PCCs) (Miller, 1993). The value of PCC higher than 0.99 is assumed to provide an acceptable certainty in a confirmation of the identity of the analyte (Strašík et al, 2003), i.e. a match of the tested (QUI in beverage) and reference (QUI in demineralised water) spectrum.…”

Section: Application In Food Analysismentioning

confidence: 99%

Comparison of column-coupled electrophoresis with liquid chromatography methods in food analysis of quinine

Veizerová

Piešťanský

Maráková

et al. 2012

Acta Facultatis Pharmaceuticae Universitatis Comenianae

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Comparison of column-coupled electrophoresis with liquid chromatography methods in food analysis of quinine (QUI) is presented in this work. The capillary isotachophoresis (CITP) on-line coupled with capillary zone electrophoresis (CZE) and hyphenated with fibre-based spectrophotometric diode array detection (DAD) was compared with, (i) high performance liquid chromatography (HPLC) method with DAD detection, and (ii) HPLC method with fluorescence detection (FD). These methods were compared through their performance parameters and determined concentrations of QUI in beverages. The concentrations of QUI in two selected bitter drinks determined by the CITP-CZE-DAD method were in a good accordance with the HPLC-DAD and HPLC-FD methods. In addition, the electrophoretic method, as well as the chromatographic methods, was able to separate potential QUI related impurities from the QUI peak. The CITP-CZE-DAD method provided excellent performance parameters that were comparable (precision, accuracy, LOD, robustness) or even better (separation efficiency) than those ones provided by the chromatographic methods. Moreover, the effectivity of the electrophoresis method was higher when considering cost of analysis (equipment, consumption of separation systems), environmental aspects (organic vs. aqueous solvents), online sample pretreatment (CITP preconcentration and sample clean-up suitable also for the more complex matrices). Considering these findings, CITP-CZE-DAD was approved as a routine automatized method for the highly reliable quality food control.

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Noncapillary Electrophoresis

Jia

et al. 2017

Encyclopedia of Analytical Chemistry

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Capillary electrophoresis (CE) is being widely applied in pharmaceutical, biological, food, chemical, environmental, and clinical fields owing to its high efficiency, universality, sensitivity, trace consumption of sample, high sample throughput, and variety of operating modes. However, it is also facing great challenges in determination and analysis of ultratrace samples, semipreparation or preparation of samples, multidimensional analysis, and coupling of multiple technologies. Load capacity is a key factor that affects the solution of these issues. It is thought that the direct increase in capillary diameter is a shortcut. Many papers and patents reported the development of various electrophoresis methods in large capillaries (>150 µm), which are uniformly named wide‐bore electrophoresis (WBE) here. This article describes the progress in WBE, including the theory, methods, applications, and so on.

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Capillary zone electrophoresis in wide bore capillary tubes with fiber-coupled diode array detection

Cited by 19 publications

References 47 publications

Enantioselective analysis of pheniramine in urine by charged CD‐mediated CZE provided with a fiber‐based DAD and an on‐line sample pretreatment by capillary ITP

Enantioselective analysis of pheniramine in urine by charged CD‐mediated CZE provided with a fiber‐based DAD and an on‐line sample pretreatment by capillary ITP

Comparison of column-coupled electrophoresis with liquid chromatography methods in food analysis of quinine

Noncapillary Electrophoresis

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