Noncapillary Electrophoresis

Abstract

Capillary electrophoresis (CE) is being widely applied in pharmaceutical, biological, food, chemical, environmental, and clinical fields owing to its high efficiency, universality, sensitivity, trace consumption of sample, high sample throughput, and variety of operating modes. However, it is also facing great challenges in determination and analysis of ultratrace samples, semipreparation or preparation of samples, multidimensional analysis, and coupling of multiple technologies. Load capacity is a key factor that affects the solution of these issues. It is thought that the direct increase in capillary diameter is a shortcut. Many papers and patents reported the development of various electrophoresis methods in large capillaries (>150 µm), which are uniformly named wide‐bore electrophoresis (WBE) here. This article describes the progress in WBE, including the theory, methods, applications, and so on.