Capillary gc-ms determination of melatonin in several pharmaceutical tablet formulations

Aboul‐Enein, Hassan Y.; Doneanu, Catalin E.

doi:10.1002/(sici)1099-0801(199902)13:1<24::aid-bmc765>3.0.co;2-i

Cited by 23 publications

(4 citation statements)

References 12 publications

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“…Melatonin was extracted using a method developed based on published protocols. [48][49][50][51] Briefly, about 300 mg of D. citri adults was homogenized to fine powder using 200 μL of ethyl acetate containing 100 mg L −1 butylated hydroxytoluene (as an antioxidant). After total homogenization, additional 550 μL of ethyl acetate was added.…”

Section: Melatonin Extraction and Purificationmentioning

confidence: 99%

Infection with phytopathogenic bacterium inhibits melatonin biosynthesis, decreases longevity of its vector, and suppresses the free radical‐defense

Nehela

Killiny

2018

Journal of Pineal Research

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Vector-borne phytopathogenic bacteria may alter the reproductive fitness, survival, behavior, and metabolism of their vectors. Candidatus Liberibacter asiaticus (CLas) is associated with the Huanglongbing (also known as citrus greening disease), one of the most destructive citrus diseases worldwide, and transmitted by Asian citrus psyllid, Diaphorina citri (Insecta, Hemiptera, Liviidae). The genome sequencing of CLas revealed that it does not have the ability to synthesize tryptophan, the precursor of melatonin, and it must acquire it from its host plant or insect vector to achieve its biologic processes, such as growth and multiplication. Herein, we aimed to develop a GC-MS-SIM-based method to detect the endogenous melatonin from small insects such as D. citri, and to explore the hidden relationship between melatonin content and D. citri-adult survival. Then, we studied the ability of exogenous melatonin supplementation to reverse the negative effects of CLas-infection. Our findings showed that CLas-infection reduced the levels of melatonin and its biosynthetic genes (DcTPHs, DcAAAD, DcSNAT, and DcASMT) of D. citri compared to uninfected insects. In addition, CLas decreased the longevity of its vector, D. citri via the suppression of the free radical-defense associated genes (SODs, GSTs, PODs, and PHGPXs). On the other hand, melatonin supplementation could reverse the negative effects of CLas-infection. Melatonin supplementation enhanced the endogenous melatonin content, melatonin biosynthetic genes, free radical-defense associated genes, and the longevity of both healthy and CLas-infected D. citri. Furthermore, melatonin supplementation decreased the CLas bacterial population within the D. citri psyllids. Based on these findings, we hypothesize that melatonin plays multi-layered defensive roles in D. citri. These roles include acting as a natural antioxidant or as an antibacterial compound.

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Section: Melatonin Extraction and Purificationmentioning

confidence: 99%

Infection with phytopathogenic bacterium inhibits melatonin biosynthesis, decreases longevity of its vector, and suppresses the free radical‐defense

Nehela

Killiny

2018

Journal of Pineal Research

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“…Although a good agreement between the two methods was obtained, a larger blood sample was needed for the headspace method (500 mL). More recently, Aboul-Enein et al (1999) reported a simple capillary GC-MS for the determination of the pineal gland hormone, melatonin, in pharmaceutical preparations. Excellent reproducibility was obtained by the splitless injection with an RSD of AE2%.…”

Section: Gc-msmentioning

confidence: 99%

Hyphenated chromatographic methods for biomaterials

Al‐Dirbashi¹,

Nakashima²

2000

Biomed. Chromatogr.

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The improvement in hyphenated analytical techniques has significantly widened their applications to the analysis of biomaterials. In this article, we discuss recent advances in applications of hyphenated chromatographic techniques including capillary electrophoresis to the analyses of biological samples. As tools of separation, gas chromatography, high-performance liquid chromatography and capillary electrophoresis are considered with special emphasis on applications utilizing the hyphenation of these methods to mass spectrometry. Moreover, applications using other detection methods such as Fourier transform infrared spectroscopy hyphenated to gas chromatography and photodiode array detector combined with high-performance liquid chromatography or capillary electrophoresis are also discussed. Owing to their high sensitivity, luminescence-based detection systems such as laserinduced fluorescence and chemiluminescence are also included in this review. Figure 3. Scheme of the coupled-column approach. C1: first analytical column; C2: second analytical column; DC: dummy column; GC: guard column; GP: gradient pump; IP: isocratic pump; IV: injection valve; MP: mobile phase reservoir; RV: Rheodyne switching valve; VV: Valco switching valve. Reprinted from Polettini et al. (1998) with permission from Elsevier Science.

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“…Over the past decades, MT analysis has gained increasing significance, where confirmatory techniques, such as radioimmunoassay [11], calorimetry [12], enzyme-linked immunoassay [13], high-resolution nuclear magnetic resonance spectroscopy [14], highperformance liquid chromatography [15,16], mass spectrometry [17,18], and electrochemical approaches [19], have been used to detect MT levels. However, owing to the low concentrations of MT (0.043 µM in the day and 0.86 µM in the night [20]) and co-existence of several endogenous metabolites in biological fluids, the accurate detection of MT at low concentrations with sufficient anti-interference ability remains a challenge.…”

Section: Introductionmentioning

confidence: 99%

Ruthenium-Anchored Carbon Sphere-Customized Sensor for the Selective Amperometric Detection of Melatonin

Jayaraman,

Rajarathinam,

Jang

et al. 2023

Biosensors

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Melatonin (MT), a pineal gland hormone, regulates the sleep/wake cycle and is a potential biomarker for neurodegenerative disorders, depression, hypertension, and several cancers, including prostate cancer and hepatocarcinoma. The amperometric detection of MT was achieved using a sensor customized with ruthenium-incorporated carbon spheres (Ru–CS), possessing C- and O-rich catalytically active Ru surfaces. The non-covalent interactions and ion–molecule adducts between Ru and CS favor the formation of heterojunctions at the sensor–analyte interface, thus accelerating the reactions towards MT. The Ru–CS/Screen-printed carbon electrode (SPCE) sensor demonstrated the outstanding electrocatalytic oxidation of MT owing to its high surface area and heterogeneous rate constants and afforded a lower detection limit (0.27 μM), high sensitivity (0.85 μA μM −1 cm−2), and excellent selectivity for MT with the co-existence of crucial neurotransmitters, including norepinephrine, epinephrine, dopamine, and serotonin. High concentrations of active biomolecules, such as ascorbic acid and tyrosine, did not interfere with MT detection. The practical feasibility of the sensor for MT detection in pharmaceutical samples was demonstrated, comparable to the data provided on the product labels. The developed amperometric sensor is highly suitable for the quality control of medicines because of its low cost, simplicity, small sample size, speed of analysis, and potential for automation.

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Capillary gc-ms determination of melatonin in several pharmaceutical tablet formulations

Cited by 23 publications

References 12 publications

Infection with phytopathogenic bacterium inhibits melatonin biosynthesis, decreases longevity of its vector, and suppresses the free radical‐defense

Infection with phytopathogenic bacterium inhibits melatonin biosynthesis, decreases longevity of its vector, and suppresses the free radical‐defense

Hyphenated chromatographic methods for biomaterials

Ruthenium-Anchored Carbon Sphere-Customized Sensor for the Selective Amperometric Detection of Melatonin

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