Capillary Filling at the Microscale: Control of Fluid Front Using Geometry

Trejo-Soto, Claudia; Costa-Miracle, E.; Rodriguez-Villarreal, Ivon; Cid, Joan; Alarcón, Tomás; Hernández–Machado, A.

doi:10.1371/journal.pone.0153559

Cited by 11 publications

(16 citation statements)

References 19 publications

(13 reference statements)

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“…The microfluidic devices used in these experiments were not intended to replicate the anatomical conditions of the human capillaries; therefore, the flexible and deformable characteristic of real blood capillaries was not considered in the fabrication process. A full description of the microfluidic device and details of the experimental method are reported in previous work [58]. Blood samples were extracted from anonymous healthy donors and delivered for our experiments from the Banc de Sang i Teixit of Barcelona, in tubes of 10 or 5 mL on an heparin based anticoagulant.…”

Section: Methodsmentioning

confidence: 99%

Normalization of Blood Viscosity According to the Hematocrit and the Shear Rate

Trejo-Soto

Hernández–Machado

2022

Micromachines

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The rheological properties of blood depend highly on the properties of its red blood cells: concentration, membrane elasticity, and aggregation. These properties affect the viscosity of blood as well as its shear thinning behavior. Using an experimental analysis of the interface advancement of blood in a microchannel, we determine the viscosity of different samples of blood. In this work, we present two methods that successfully normalize the viscosity of blood for a single and for different donors, first according to the concentration of erythrocytes and second according to the shear rate. The proposed methodology is able to predict the health conditions of the blood samples by introducing a non-dimensional coefficient that accounts for the response to shear rate of the different donors blood samples. By means of these normalization methods, we were able to determine the differences between the red blood cells of the samples and define a range where healthy blood samples can be described by a single behavior.

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Section: Methodsmentioning

confidence: 99%

Normalization of Blood Viscosity According to the Hematocrit and the Shear Rate

Trejo-Soto

Hernández–Machado

2022

Micromachines

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“…The microchannel is molded in a biocompatible hydrophobic silicone, PDMS (polydimethylsiloxane), on a glass substrate according to replica moulding and soft lithography microfabrication methods. 34,36 The bottom surface of the microchannel is made of glass and the top and lateral surfaces are made of PDMS. In our experiments, we use human blood of varying ages (defined as the time elapsed since extraction) and haematocrits (the volume fraction occupied by the RBCs).…”

Section: Experimental Set-up and Measurementsmentioning

confidence: 99%

“…The analysis presented in this paper is a generalisation to non-Newtonian fluids of the one presented in ref. 34. In particular, we define a new quantity associated with this non-Washburn regime, the front shear rate, which allows us to characterise the non-linear rheology of non-Newtonian fluids.…”

Section: Introductionmentioning

confidence: 99%

Front microrheology of the non-Newtonian behaviour of blood: scaling theory of erythrocyte aggregation by aging

et al. 2017

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We introduce a new framework to study the non-Newtonian behaviour of fluids at the microscale based on the analysis of front advancement. We apply this methodology to study the non-linear rheology of blood in microchannels. We carry out experiments in which the non-linear viscosity of blood samples is quantified at different haematocrits and ages. Under these conditions, blood exhibits a power-law dependence on the shear rate. In order to analyse our experimental data, we put forward a scaling theory which allows us to define an adhesion scaling number. This theory yields a scaling behaviour of the viscosity expressed as a function of the adhesion capillary number. By applying this scaling theory to samples of different ages, we are able to quantify how the characteristic adhesion energy varies as time progresses. This connection between microscopic and mesoscopic properties allows us to estimate quantitatively the change in the cell-cell adhesion energies as the sample ages.

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“…The theoretical model used assumes that velocity inside the microchannel behaves according to Darcy’s law. In past experiments [ 34 ], Trejo C. observed that this theoretical description of the velocity of the fluid inside the microchannel held for microchannels of 1 mm width and heights over 150 um. To test distinct aspect ratios, the authors considered microchannels of different heights: b = 300 μm, b = 200 μm, b = 150 μm, and b = 50 μm; width w = 1 mm; and length lc = 4 cm.…”

Section: Methodsmentioning

confidence: 98%

Microrheometer for Biofluidic Analysis: Electronic Detection of the Fluid-Front Advancement

et al. 2021

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The motivation for this study was to develop a microdevice for the precise rheological characterization of biofluids, especially blood. The method presented was based on the principles of rheometry and fluid mechanics at the microscale. Traditional rheometers require a considerable amount of space, are expensive, and require a large volume of sample. A mathematical model was developed that, combined with a proper experimental model, allowed us to characterize the viscosity of Newtonian and non-Newtonian fluids at different shear rates. The technology presented here is the basis of a point-of-care device capable of describing the nonlinear rheology of biofluids by the fluid/air interface front velocity characterization through a microchannel. The proposed microrheometer uses a small amount of sample to deliver fast and accurate results, without needing a large laboratory space. Blood samples from healthy donors at distinct hematocrit percentages were the non-Newtonian fluid selected for the study. Water and plasma were employed as testing Newtonian fluids for validation of the system. The viscosity results obtained for the Newtonian and non-Newtonian fluids were consistent with pertinent studies cited in this paper. In addition, the results achieved using the proposed method allowed distinguishing between blood samples with different characteristics.

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Capillary Filling at the Microscale: Control of Fluid Front Using Geometry

Cited by 11 publications

References 19 publications

Normalization of Blood Viscosity According to the Hematocrit and the Shear Rate

Normalization of Blood Viscosity According to the Hematocrit and the Shear Rate

Front microrheology of the non-Newtonian behaviour of blood: scaling theory of erythrocyte aggregation by aging

Microrheometer for Biofluidic Analysis: Electronic Detection of the Fluid-Front Advancement

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