Capillary electrophoretic separation of organophosphonic acids using borate esterification and direct UV detection

In situ determination of nerve agents in various matrices by portable capillary electropherograph with contactless conductivity detection

“…Alternative electrolyte probes applied in indirect UV detection include sorbate [36,37], borate [38,39] and chromate [40]. Derivatization with sodium borate and direct UV detection at 214 or 254 nm was advanced by Robins and Wright [39]. All of the reported research on CE with UV detection relies on laboratory-based instruments with UV detectors that consume large quantities of energy.…”

“…Phenylphosphonic acid was used as the UV-absorbing probe, as most NA degradation products do not absorb in UV, and this technique was subsequently adopted by other authors [33][34][35]. Alternative electrolyte probes applied in indirect UV detection include sorbate [36,37], borate [38,39] and chromate [40]. Derivatization with sodium borate and direct UV detection at 214 or 254 nm was advanced by Robins and Wright [39].…”

Three-phase hollow fiber liquid-phase microextraction of organophosphorous nerve agent degradation products from complex samples

“…This step of derivatization is still time-consuming and can suffer from matrix effects in real samples [5]. Capillary electrophoresis coupled to mass spectrometry can be used for the direct determination of these compounds [6] but this technique is not sensitive enough, considering its use for the determination of AMPAs at trace level [7][8][9][10][11][12][13]. An alternative to GC consists in the use of liquid chromatography [14].…”

Determination of small phosphorus-containing compounds by capillary electrophoresis

“…In order to eliminate the problem of non-selectivity of indirect UV and fluorescence detection, Robins and Wright developed the direct UV detection of AA based on the formation of borate-phosphonic acid esters [58]. Complexation occurred in 10 mM sodium tetraborate solution at pH 7.0 or higher and nanogram concentrations of AA were detected but no realsamples were analyzed.…”