Capillary electrophoresis with indirect UV detection for the determination of stabilizers and citrates present in human albumin solutions

Jaworska, Małgorzata; Cygan, Paulina; Wilk, Małgorzata; Anuszewska, Elżbieta

doi:10.1016/j.jpba.2009.03.031

Cited by 9 publications

(2 citation statements)

References 20 publications

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“…Samples ranging from human albumin solutions [25], plasma and serum for proteome analysis [26] to urine with metabolized MRI contrast agents [27] have all been analyzed by CE. The high sensitivity coupled with the small amount of sample required for analysis and the robustness of CE make it particularly applicable to clinical testing.…”

Section: Introductionmentioning

confidence: 99%

Analysis of neurotrophins in human serum by immunoaffinity capillary electrophoresis (ICE) following traumatic head injury

Kalish

Phillips

2010

Journal of Chromatography B

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Neurotrophins, including brain-derived neurotrophic factor (BDNF), ciliary neurotrophic factor (CNTF), neurotrophin-3 (NT-3), neurotrophin-4 (NT-4), and β-nerve growth factor (β-NGF), play an active role in the development, maintenance and survival of cells of the central nervous system (CNS). Previous research has indicated that a decrease in concentrations of these neurotrophins is often associated with cell death and ultimately patient demise. However, much of the research conducted analyses of samples taken directly from the CNS, i.e., of samples that are not readily available in clinical trauma centers. In an attempt to obtain a method for evaluating neurotrophins in a more readily accessible matrix, i.e., serum, a precise and accurate immunoaffinity capillary electrophoresis (ICE) method was developed and applied to measure neurotrophins in serum from patients with various degrees of head injury. The five neurotrophins of interest were extracted and concentrated by specific immunochemically immobilized antibodies, bound directly to the capillary wall, and eluted and separated in approximately 10 min. NT-3, BDNF, CNTF and β-NGF showed a marked decrease in concentration as the severity of the head injury increased: mild versus severe: 91% decrease for NT-3; 93 % decrease for BDNF; 93 % decrease for CNTF; and a 87 % decrease for β-NGF. This decrease in concentration is consistent with the neuroprotective roles that neurotrophins play in the maintenance and survival of neuronal cells. The results obtained by the ICE method were closely comparable with those generated by a commercially available ELISA method.

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Section: Introductionmentioning

confidence: 99%

Analysis of neurotrophins in human serum by immunoaffinity capillary electrophoresis (ICE) following traumatic head injury

Kalish

Phillips

2010

Journal of Chromatography B

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“…Usually, citrates are analyzed using an indirect procedure necessitating their complexation with transition metal ions [ 10 – 12 ] or enzymatic derivation to achieve clear absorption bands, preferably in the visible range [ 13 , 14 ]. Rather than the spectrophotometric method, chromatographic analysis [ 6 , 15 – 18 ], capillary electrophoresis [ 19 , 20 ], or classical titration [ 18 ] are typically used.…”

Section: Introductionmentioning

confidence: 99%

Convenient UV-spectrophotometric determination of citrates in aqueous solutions with applications in the pharmaceutical analysis of oral electrolyte formulations

Krukowski

Karasiewicz

Kołodziejski

2017

Journal of Food and Drug Analysis

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Herein, we present a convenient method for quantitative spectrophotometric determination of citrate ions in aqueous solutions in the middle-UV range. It involves measuring the absorbance of citric acid at 209 nm under suppressed dissociation at pH < 1.0 in the presence of hydrochloric acid. Validation of the method was performed according to the guidelines of the International Conference on Harmonization. A very good linear dependence of the absorbance on concentration (r = 0.9999) was obtained in a citrate concentration range of 0.5-5.0 mmol/L. This method is characterized by excellent precision and accuracy; the coefficient of variation in each case is below the maximal permissible value (%RSD < 2). The proposed analytical procedure has been successfully applied to the determination of citrates in oral electrolyte formulations.

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Citrate analysis using capillary electrophoresis and complexation with Eu3+-tetracycline

2018

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Capillary electrophoresis with indirect UV detection for the determination of stabilizers and citrates present in human albumin solutions

Cited by 9 publications

References 20 publications

Analysis of neurotrophins in human serum by immunoaffinity capillary electrophoresis (ICE) following traumatic head injury

Analysis of neurotrophins in human serum by immunoaffinity capillary electrophoresis (ICE) following traumatic head injury

Convenient UV-spectrophotometric determination of citrates in aqueous solutions with applications in the pharmaceutical analysis of oral electrolyte formulations

Citrate analysis using capillary electrophoresis and complexation with Eu3+-tetracycline

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