Capillary electrophoresis to investigate biopharmaceuticals and pharmaceutically-relevant binding properties

Deeb, Sami El; Wätzig, Hermann; El‐Hady, Deia Abd

doi:10.1016/j.trac.2013.04.005

Cited by 50 publications

(64 citation statements)

References 79 publications

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“…ACE is an excellent and highly precise option to estimate binding properties, if the binding interaction is combined with a change in charge of the interacting partners [96][97][98][99] (Fig. 7).…”

Section: Affinity Capillary Electrophoresis (Ace)mentioning

confidence: 99%

“…Typically percent relative standard deviations of migration data within one measurement series of \1 % are found, the variability of the binding constants probably being in the same range [101][102][103][104]. Comprehensive information about method development and successful examples has recently been reviewed [96][97][98][99].…”

Section: Affinity Capillary Electrophoresis (Ace)mentioning

confidence: 99%

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Data quality in drug discovery: the role of analytical performance in ligand binding assays

Wätzig

Oltmann-Norden

Steinicke

et al. 2015

J Comput Aided Mol Des

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Despite its importance and all the considerable efforts made, the progress in drug discovery is limited. One main reason for this is the partly questionable data quality. Models relating biological activity and structures and in silico predictions rely on precisely and accurately measured binding data. However, these data vary so strongly, such that only variations by orders of magnitude are considered as unreliable. This can certainly be improved considering the high analytical performance in pharmaceutical quality control. Thus the principles, properties and performances of biochemical and cell-based assays are revisited and evaluated. In the part of biochemical assays immunoassays, fluorescence assays, surface plasmon resonance, isothermal calorimetry, nuclear magnetic resonance and affinity capillary electrophoresis are discussed in details, in addition radiation-based ligand binding assays, mass spectrometry, atomic force microscopy and microscale thermophoresis are briefly evaluated. In addition, general sources of error, such as solvent, dilution, sample pretreatment and the quality of reagents and reference materials are discussed. Biochemical assays can be optimized to provide good accuracy and precision (e.g. percental relative standard deviation <10 %). Cell-based assays are often considered superior related to the biological significance, however, typically they cannot still be considered as really quantitative, in particular when results are compared over longer periods of time or between laboratories. A very careful choice of assays is therefore recommended. Strategies to further optimize assays are outlined, considering the evaluation and the decrease of the relevant error sources. Analytical performance and data quality are still advancing and will further advance the progress in drug development.

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Section: Affinity Capillary Electrophoresis (Ace)mentioning

confidence: 99%

Section: Affinity Capillary Electrophoresis (Ace)mentioning

confidence: 99%

Data quality in drug discovery: the role of analytical performance in ligand binding assays

Wätzig

Oltmann-Norden

Steinicke

et al. 2015

J Comput Aided Mol Des

Self Cite

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“…CE appeared in the 1980s and was rapidly developed due to transition to the silica capillaries with very small and regular diameters (tens of micrometers) and application of the straight detection in the fluid flow [22]. The CE and related electroseparation techniques are widely used for the estimation of stability constants because of rapidity, low cost of reagents, and high performance [23][24][25][26][27][28][29][30][31]. To study the interactions between a metal and ligand, several electropherograms are recorded with varying ligand content in background electrolytes (BGE) and metal solution injected as a sample.…”

Section: Introductionmentioning

confidence: 99%

Composition and stability constants of copper(II) complexes with succinic acid determined by capillary electrophoresis

Sursyakova

Burmakina

Rubaylo

2016

Journal of Coordination Chemistry

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Fax: 73912494108 2 Composition and stability constants of copper(II) complexes with succinic acid ions determined by capillary electrophoresisThe advantage of usage of capillary electrophoresis technique was demonstrated for studying a complicated system owing to the dependence of direction and velocity of the electrophoretic movement on the charge of complex species. The stability constants of copper(II) complexes with ions of succinic acid were determined by capillary electrophoresis, including the 1:2 metal to ligand complexes which are rarely mentioned and taken into account in studies during recent years. The measurements were carried out at 25 ºC and ionic strength of 0.1, obtained by mixing the solutions of succinic acid and lithium hydroxide up to рН 4.2 -6.2. It was shown that while pH was more than 4.5 the zone of copper(II) complexes with succinate ions moves as an anion. It is impossible to treat this fact using only the complexes with a metal-ligand ratio of 1:1 (CuL 0 , CuHL + ).The following values of stability constants were obtained: log β(CuL) = 2.89 ± 0.02, log β(CuHL + ) = 5.4 ± 0.5, log β(CuL 2 2-) = 3.88 ± 0.05, log β(CuHL 2 -) = 7.2 ± 0.3.

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“…Its major advantages include high separation efficiency, ease of method optimization, the possibility to analyze small sample volumes, the use of low toxic organic solvents volume and inexpensive capillaries. Although, the sensitivity is lower compared to LC, tremendous improvements can be obtained using different preconcentration methods [3,4].…”

Section: Introductionmentioning

confidence: 99%

Capillary Electrophoresis as a Promising Technique to Evaluate Metabolites Secreted by Fungal Biocontrol Agents

et al. 2016

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Capillary electrophoresis to investigate biopharmaceuticals and pharmaceutically-relevant binding properties

Cited by 50 publications

References 79 publications

Data quality in drug discovery: the role of analytical performance in ligand binding assays

Data quality in drug discovery: the role of analytical performance in ligand binding assays

Composition and stability constants of copper(II) complexes with succinic acid determined by capillary electrophoresis

Capillary Electrophoresis as a Promising Technique to Evaluate Metabolites Secreted by Fungal Biocontrol Agents

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