Capillary electrophoresis on microchip

Dolnı́k, Vladislav; Liu, Shaorong; Jovanovich, Stevan

doi:10.1002/(sici)1522-2683(20000101)21:1<41::aid-elps41>3.0.co;2-7

Cited by 478 publications

(339 citation statements)

References 128 publications

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“…(3) With the combination of Equations 2 and 3, K d is determined by Equation 4 [26]. (4) where the values of P 0 and A 0 are known.…”

Section: Principle Of K D Determination Using Famentioning

confidence: 99%

“…Microchip capillary electrophoresis (CE) coupled with laser-induced fluorescence (LIF) detection offers CE separations with speed, sensitivity, ease of automation and low consumption of reagents [1][2][3]. Many techniques developed in traditional CE have been transferred onto microchip CE with appropriate modifications [3][4][5][6][7].…”

Section: Introductionmentioning

confidence: 99%

“…Many techniques developed in traditional CE have been transferred onto microchip CE with appropriate modifications [3][4][5][6][7]. Multilane microchips have been developed to obtain high throughput analysis and were applied in DNA sequencing, genotyping, pharmaceuticals and other areas [8][9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Frontal analysis in microchip CE: A simple and accurate method for determination of protein–DNA dissociation constant

et al. 2007

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Equilibrium constants, such as the dissociation constant (K d ), are a key measurement of noncovalent interactions that are of importance to the proper functioning of molecules in living systems. Frontal analysis (FA) is a simple and accurate capillary electrophoresis (CE) method for the determination of K d . Microchip CE coupled with laser-induced fluorescence (LIF) detection was used to determine K d of protein-DNA interactions using the FA method. A model system of immunoglobulin E (IgE) and the IgE-binding aptamer was selected to demonstrate the capability of FA in microchip CE. Because the fluorescence emission was dependent on the dye migration velocity, the velocity of the free aptamer was adjusted to be the same as that of the aptamer-IgE complex by setting up individual separation voltage configurations for the free and bound aptamers. The ratio of the free and bound aptamers in the equilibrium mixture was directly measured from the heights of their plateaus detected at 1.0 cm from the intersection of the microchip, and no internal standard was needed. The K d of the IgE-aptamer pair was determined as 6 ± 2 nM which is consistent with the reported results (8 nM).

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“…(3) With the combination of Equations 2 and 3, K d is determined by Equation 4 [26]. (4) where the values of P 0 and A 0 are known.…”

Section: Principle Of K D Determination Using Famentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Frontal analysis in microchip CE: A simple and accurate method for determination of protein–DNA dissociation constant

et al. 2007

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“…Microchip electrophoresis has received considerable interest in the analytical chemistry due to the intrinsic characteristics of high speed, high throughput, easy integration, low consumption of samples and reagents, miniaturization, and automation [19][20][21]. This approach has been successfully used to analyze DNA fragments [22][23][24][25], amino acids [26], proteins [27,28], and carbohydrates [29].…”

Section: Introductionmentioning

confidence: 99%

Determination of human blood glucose levels using microchip electrophoresis

et al. 2007

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Determination of human blood glucose levels using microchip electrophoresisA high-performance monitoring system for human blood glucose levels was developed using microchip electrophoresis with a plastic chip. The combination of reductive amination as glucose labeling with fluorescent 2-aminoacridone (AMAC) and glucose-borate complex formation realized the highly selective detection of glucose even in a complex matrix such as a blood sample. The migration time of a single peak, observed on an electropherogram of AMAC-labeled plasma, closely resembled that of glucose standard solution. The treatment of plasma with hexokinase or glucokinase for glucose phosphorylation resulted in a peak shift from approximately 145 to 70 s, corresponding to glucose and glucose-6-phosphate, respectively. A double-logarithm plot revealed a linear relationship between glucose concentration and fluorescence intensity in the range of 1-300 mM of glucose (r 2 = 0.9963; p ,0.01), and the detection limit was 0.92 mM. Furthermore, blood glucose concentrations estimated from the standard curves of three subjects were compared with results obtained by conventional colorimetric analysis using glucose dehydrogenase. Good correlation was observed between methods according to simple linear regression analysis (p ,0.05). The reproducibility of the assay was about 6.3-9.1% (RSD) and the within-days and between-days reproducibility were 1.6-8.4 and 5.2-7.2%, respectively. This system enables us to determine blood glucose with high sensitivity and accuracy, and will be applicable to clinical diagnosis.

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“…Microfluidic devices have been applied for electrophoretic separations for a variety of biochemical and chemical analysis [1][2][3][4][5][6][7]. Latest developments in microchip electrophoresis (MCE) have been summarized in recent reviews [8][9][10][11][12][13]. MCE is often regarded as a higher miniaturized version of capillary electrophoresis (CE) and a lot of applications can easily be transferred from classical CE to the microchip format.…”

Section: Introductionmentioning

confidence: 99%

Surface modification in microchip electrophoresis

2003

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Different approaches and techniques for surface modification of microfluidic devices applied for microchip electrophoresis are reviewed. The main focus is on the improved electrophoretic separation by reducing analyte-wall interactions and manipulation of electroosmosis. Approaches and methods for permanent and dynamic surface modification of microfluidic devices, manufactured from glass, quartz and also different polymeric substrates, are described.

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Capillary electrophoresis on microchip

Cited by 478 publications

References 128 publications

Frontal analysis in microchip CE: A simple and accurate method for determination of protein–DNA dissociation constant

Frontal analysis in microchip CE: A simple and accurate method for determination of protein–DNA dissociation constant

Determination of human blood glucose levels using microchip electrophoresis

Surface modification in microchip electrophoresis

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