Capillary electrophoresis of subcellular-sized particles

Radko, Sergey P.; Chrambach, Andreas

doi:10.1016/s0378-4347(98)00307-7

Cited by 56 publications

(62 citation statements)

References 43 publications

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“…CE is a relatively nonperturbing method allowing the structure-dependent electrophoretic separation of monodisperse or polydisperse mixtures of small molecules [339][340][341], macromolecules [342][343][344][345], polymers [346][347][348][349] and colloids [350], up to nanoparticles [351][352][353][354] and liposomes [355], even bacteria [350,356] and viruses, in various modes of gentle separation techniques using nonaqueous or aqueous buffers with coated or uncoated capillary columns. The small volume of the analyte needed in CE experiments has made possible the measurement of the cellular contents (native proteins [357], specific fluorescent-labelled or-induced proteins [358], metabolites), the monitoring of single cells [359,360] or bacteria communications [361].…”

Section: Discussionmentioning

confidence: 99%

Capillary electrophoresis – mass spectrometry: 15 years of developments and applications

Schmitt‐Kopplin¹,

Frommberger²

2003

Electrophoresis

281

232

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Capillary electrophoresis -mass spectrometry: 15 years of developments and applicationsSince its introduction in 1987, capillary electrophoresis-mass spectrometry (CE-MS) has developed to a well accepted multidimensional analytical approach complementary and/or competitive to classical MS-hyphenated separation techniques. The threefold combination of rapid developments of an exceptional separation technique, of selective mass detection possibilities, and of very mild ionization modes first allowed these progresses. This article shows the CE specificities that need to be well controlled/known, compared to classical and more routinely used liquid chromatography in the light of its coupling to MS. The major trends and developments over the last 15 years and most of the reviews and applications found in ISI Web of science and publisher databases are presented in a tabulated way. The reader can thus rapidly find existing CE-MS analysis techniques in his field of research and application (forensics, environment, bioanalytics, pharmaceutics, and metabolites).

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Section: Discussionmentioning

confidence: 99%

Capillary electrophoresis – mass spectrometry: 15 years of developments and applications

Schmitt‐Kopplin¹,

Frommberger²

2003

Electrophoresis

281

232

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“…Although flow cytometry was historically used first to detect individual organelles [9,10], CE has the additional analytical advantage of being able to separate organelles based on their electrophoretic mobilities [11][12][13][14]. In general, the electrophoretic mobility of a particle stems from its size [12,14,19] and surface characteristics [13,19], which makes CE useful for comparing the size or surface characteristics of individually detected biological species, such as proteins [15], mitochondria [8,16], nuclei [17], and acidic organelles [18]. The size and surface characteristics of these particles may be highly heterogeneous, making it extremely important to analyze samples with a sufficient number of individual species so that an accurate statistical description is observed.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of individual particle capillary electrophoresis experiments via quantile analysis

Whiting

Arriaga

2007

Journal of Chromatography A

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The number of particles in a sample heavily influences the shape of the distribution describing the corresponding individual particle measurements. Selecting an adequate number of particles that prevents biases due to sample size is particularly difficult for complex biological systems in which statistical distributions are not normal. Quantile analysis is a powerful statistical technique that can rapidly compare differences between multiple distributions of individual particles. This report utilizes quantile analysis to show that the number of events detected affects the mobility distributions for rat liver and mouse liver mitochondria, sample individual particles, when analyzed via capillary electrophoresis with laser-induced fluorescence. When the mitochondrial sample is small (e.g. <78), there are not enough events to obtain statistically relevant mobility data. Adsorption to the capillary surface also significantly affects the mobility distribution at a small number of events in uncoated and dynamically coated capillaries. These adsorption effects can be overcome when the mitochondrial load on the capillary is sufficiently large (i.e. >609 and >1426 events for mouse liver on uncoated capillaries and rat liver on dynamically coated capillaries, respectively). It is anticipated that quantile analysis can be used to study other distributions of individual particles, such as nanoparticles, organelles, and biomolecules, and that distributions of these particles will also be dependant on sample size.

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“…. 31,32 Therefore, the main driving force for the larger-sized negatively charged Au NPs 33 to move faster toward the anodic end (detector side) was probably their more pronounced relaxation effect 20,[34][35][36] during the application of the REPSM. In addition, I noticed that the peak widths of the signals in the electropherograms for both sizes of Au NPs were reduced slightly when the REPSM voltage processes were applied, suggesting that the Au NPs in the sample underwent stacking during the application of the REPSM; the finding is consistent with those of previous reports.…”

Section: Mechanism Of On-line Concentration and Separation Of Au Npsmentioning

confidence: 99%

Monitoring the On-line Concentration and Separation of Gold Nanoparticles Using the Reversed Electrode Polarity Stacking Mode and Micellar Electrokinetic Chromatography

Liu

2010

ANAL. SCI.

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Capillary electrophoresis of subcellular-sized particles

Cited by 56 publications

References 43 publications

Capillary electrophoresis – mass spectrometry: 15 years of developments and applications

Capillary electrophoresis – mass spectrometry: 15 years of developments and applications

Evaluation of individual particle capillary electrophoresis experiments via quantile analysis

Monitoring the On-line Concentration and Separation of Gold Nanoparticles Using the Reversed Electrode Polarity Stacking Mode and Micellar Electrokinetic Chromatography

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