Capillary electrophoresis of abnormal hemoglobins associated with α-thalassemias

Separation of hemoglobin variants with similar charge by capillary isoelectric focusing: Value of isoelectric point for identification of common and uncommon hemoglobin variants

“…Low levels of Hb A 2 are also precisely measured by CIEF, but the use of these data for the detection of mild a-thalassemia syndromes has not been studied. CIEF can, however, identify Hb H and thus detect the more severe forms of a-thalassemia that cause Hb H disease [12,14].…”

Screening of umbilical cord blood hemoglobins by isoelectric focusing in capillaries

“…Curiously, there are no corresponding separations of globin chains by CZE-IEF. The only one reported is in fact a free-zone electrophoresis separation in pH 2.5 buffer, in presence of 7 M urea and 0.1 O/ o Triton X-100 [17]. The same authors I181 also offer a full separation between a, B and y chains .…”

“…What can be achieved in a corresponding separation by CZE-IEF has been shown by Zhu etal. [17,191: Hb F and A are fully resolved (as well as the homotetramer Hb Bart) and clearly labeled in their figures. However, the series of peaks migrating after Hb A (17-18 min) have not been identified, although the authors suggest that they 'probably represent glycated forms of Hb A, acetylated Hb F and degradation products'.…”

CE‐based analysis of hemoglobin and its applications in clinical analysis

“…The frequently employed wavelengths for the CE-UV detection are around 210 nm, 280 nm and 415 nm. Zhu et al [37][38][39] had early found that when scanning detection performed in the 200-360 nm range, a series of extra peaks could be observed in the low UV region due to the ampholyte absorption. While, by using scanning detection in the UV region above 280 nm, spectral data can be obtained for distinguishing different variants such as Hb A and its structural variants.…”

“…When they occur in sequence, it is defined as two-step CIEF and occur simultaneously as one-step CIEF. Two-step CIEF with chemical mobilization [37][38][39] or pressure mobilization [22,23], and the onestep CIEF [33,34] was applied as both qualitative and quantitative analysis of Hbs. Mario et al [31] compared the two methods and found that the two-step assay obtained a much higher resolution than the one-step , and b E , were well resolved, as shown in Fig.…”