Separation of hemoglobin variants with similar charge by capillary isoelectric focusing: Value of isoelectric point for identification of common and uncommon hemoglobin variants

Insights into the nature of uranium target proteins within zebrafish gills after chronic and acute waterborne exposures

“…The presence of hemoglobin subunits was confirmed by both bottom-up and topdown ( Figure 2) analysis of the off-gel IEF fractions with pI > 6 (> 90% sequence coverage). The data were consistent with the high iron content of these fractions and the theoretical pIs of hemoglobin subunits ranging from 6.6 to 8 [17]. The coelution of U and hemoglobin subunits during RPLC(C 4 ) analysis strongly suggested that hemoglobin is 1 of the U carriers in zebrafish blood (Figure 2).…”

Comparison between capillary zone electrophoresis and capillary isoelectric focusing for thalassemia screening in southern China

“…IEF is a technique which has extremely high resolution and has the potential to separate analytes with only a single charge difference. Research indicates that CIEF exhibits both diagnostic sensitivity and specific criterion of Hb variant identification and that isoelectric point is an objective and specification criterion of Hb variant identification . Helena V8 is the only automated multichannel CE system to use isoelectric focusing for the separation and quantitation of hemoglobin by CE.…”

“…Research indicates that CIEF exhibits both diagnostic sensitivity and specific criterion of Hb variant identification and that isoelectric point is an objective and specification criterion of Hb variant identification. 11 Helena V8 is the only automated multichannel CE system to use isoelectric focusing for the separation and quantitation of hemoglobin by CE. But in our study, it did not exhibit enough sensitivity of trace peaks identification.…”

Separation of haemoglobin HbE and HbA2 by the fully automated, high‐pressure liquid chromatography Tosoh HLC‐723 G7 analyzer

“…Reports from previous findings have recognized that the pIs of many clearly appreciable Hb variants can differ for as low as 0.025 units. Among these, the group of Hbs with pI around 7.410 units is particularly crowded, with HbE, HbC‐Harlem and HbO‐Arab besides HbA 2 (Hempe & Craver, 2000). As a differentiation of HbE with either HbC‐Harlem or HbO‐Arab can be established by using appropriate tests, the problem remains with HbA 2 , as above‐mentioned.…”