Capillary electrophoresis in drug analysis

Thormann, Wolfgang; Caslavska, Jitka

doi:10.1002/elps.1150191602

Cited by 24 publications

(9 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The concentration sensitivity of UV-visible absorbance detection in CE is, however, relatively poor compared with that in HPLC. CE has become an advanced analytical methods for drug analysis in pharmaceutical, therapeutic, diagnostic, and forensic applications [12]. Reliable and automated CE instruments are commercially available and have promoted the exploration of an increasing number of CE methods for drug analysis inseveralmatrixes [13 17].…”

Section: Introductionmentioning

confidence: 99%

Capillary zone electrophoretic determination of tosufloxacin and trovafloxacin in urine

et al. 2002

View full text Add to dashboard Cite

Key WordsCapillary zone electrophoresis Tosufloxacin and trovafloxacin Antibacterials Urine samples SummaryA simple and rapid capillaryzone electrophoretic method with UV detection has been developed for determination of tosufloxacin and trovafloxacin. The separation was performed in fused-silica capillaries (57 cm length x 75 I~m i.d.); the running bufferwas 35 mM borate + 35 mM phosphate buffer solution, pH 8.6, containing 6% (v/v) acetonitrile. The applied potential was 15 kV, the temperature 30 ~ and detection was at 262 nm. Piromidic acid was used as the internal standard. Response was linearly dependent on concentration in the range 1.0-120.0 I~g mL 1 and the detection hmitwas 0.2 I~g mL 1 for both compounds.The analysis was highly reproducible (RSD bel',,veen 3.41 and 1.25%). The method was applied to the determination of tosufloxacin and trovafloxacin in human and rat urine. The method was validated by using HPLC as a reference method. Recoverywas bel ',,veen 96.8 and 102%.

show abstract

Section: Introductionmentioning

confidence: 99%

Capillary zone electrophoretic determination of tosufloxacin and trovafloxacin in urine

et al. 2002

View full text Add to dashboard Cite

show abstract

“…It offers attractive features such as high efficiency and resolution, and one of the major advantages that makes it both competitive and a good alternative over other separation techniques is the ability of CE to separate both charged and neutral molecules [16,17]. In addition, CE is an easy to use and low-cost method in comparison to HPLC.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of a capillary electrophoresis method for routine determination of varenicline tartrate in quality control laboratories

Çelebi̇er

Koçak

Altınöz

2015

Maced. J. Chem. Chem. Eng.

View full text Add to dashboard Cite

In this study, analyses were carried out on a fused-silica capillary (i.d. 50.0 µm, total length 48.5 cm, and effective length 40.0 cm) in normal mode by applying a voltage of 20 kV. Sample injections were made in hydrodynamic mode over 7 s under a pressure of 50 mbar. The capillary temperature was set at 35 ºC and the detection was performed at a wavelength of 205 nm. The background electrolyte was 40 mM citrate buffer at pH 6.0 and the internal standard was labetalol hydrochloride. The total analysis time was shorter than 5 minutes. The method was validated according to the International Conference on Harmonization (ICH) guidelines, and the method was found to be linear, precise, accurate, specific, robust, and rugged. The linearity range was found to be 1.0-60.0 µg ml -1 and the limit of detection and quantitation were 0.5 and 1.0 µg ml -1 , respectively. The developed method was proposed for quality-control laboratories.Keywords: capillary electrophoresis; varenicline tartrate; analytical method validation; pharmaceutical dosage forms; quality control ЕВАЛУАЦИЈА НА МЕТОДОТ НА КАПИЛАРНА ЕЛЕКТРОФОРЕЗА ЗА РУТИНСКО ОПРЕДЕЛУВАЊЕ ВАРЕНИКЛИН ТАРТАРАТ ВО ЛАБОРАТОРИИ ЗА КОНТРОЛА НА КВАЛИТЕТВо оваа студија извршена е анализа на капилара од фузирана силика (в.д. 50.0 µm, должина 48,5 cm и ефективна должина 40,0 cm) во нормален мод со примена на напон од 20 kV. Инјектирањето на примероците беше вршено во хидродинамичен мод во времетраење од 7 s под притисок од 50 mbar. Температурата на капиларата беше на 35 ºC и детекцијата беше вршена на бранова должина од 205 nm. Беше користен 40 mM цитратен пуфер на pH 6.0 како електролит на матрицата, додека како внатрешен стандард беше користен лабеталол-хидрохлорид. Вкупното време на анализа беше под 5 минути. Методот беше валидиран според препораките на Меѓународната конференција за хармонизација (International Conference on Harmonization -ICH) при што беше утврдено дека методот е линеарен, прецизен, точен, специфичен, робустен и отпорен. Линеарниот опсег изнесува 1.0-60.0 µg ml -1 , додека прагот на детекција и квантификација изнесуваа соодветно 0.5 и 1.0 µg ml -1 . Разработениот метод е предложен за лаборатории за контрола на квалитет.Клучни зборови: капиларна електрофореза; вареницилин тартарат; валидација на аналитички метод; фармацевтско дозирање; контрола на квалитет

show abstract

“…Since the commercial availability of CE in the late 1980s, the CE applications have moved from the laboratory of analytical chemists to the laboratories of the clinical chemists [47,60,73,74]. CE is useful as clinical tool in the diagnosis and course of metabolic disorders such as phenylketonuria [75], homocystinuria or glutathione synthase deficiency [16] or porphyria [47].…”

Section: Pharmaceutical Clinical and Forensic Applicationsmentioning

confidence: 99%

Biomedical applications of capillary electrophoresis with laser‐induced fluorescence detection

Páez

Hernández

2001

Biopharm & Drug Disp

View full text Add to dashboard Cite

Capillary electrophoresis (CE) is a high-efficiency analytical technique that has had a great impact as a tool in biomedical research, clinical and forensic practice in the last ten years. Only in one of the applications, the DNA analysis, it has had an explosive exponential growth in the last few years. This impact is expressed in an enormous amount of CE articles and many reviews. The CE advantages with respect to other analytical techniques: the required very small sample volume, rapid analysis, great resolution power and low costs, have made this technique ideal for the analysis of a numerous endogenous and exogenous substances present in biological fluids. The different modes of CE have been coupled to different detection techniques such as UV-absorbance, electrochemical, mass spectrometry and laser-induced fluorescence detection (LIFD) to detect different nature and molecular size separated analytes. This review focuses mostly on the applications of CE-LIFD, to measure drugs and endogenous neuroactive substances such as amino acids and monoamines, especially in microdialysis samples from experimental animals and humans. CE-LIFD trends are discussed: automated faster analysis with capillary array systems, resolution power improvement, higher detection sensitivity, and CE systems miniaturization for extremely small sample volume, in order to make CE easier and affordable to the lab bench or the clinical bed.

show abstract

Capillary electrophoresis in drug analysis

Cited by 24 publications

References 37 publications

Capillary zone electrophoretic determination of tosufloxacin and trovafloxacin in urine

Capillary zone electrophoretic determination of tosufloxacin and trovafloxacin in urine

Evaluation of a capillary electrophoresis method for routine determination of varenicline tartrate in quality control laboratories

Biomedical applications of capillary electrophoresis with laser‐induced fluorescence detection

Contact Info

Product

Resources

About