Capillary electrophoresis fingerprinting coupled with chemometrics to evaluate the quality consistency and predict the antioxidant activity of Sanhuang tablet as part of its quality control

Abstract

A capillary electrophoresis fingerprint was constructed for Sanhuang tablet, a Chinese traditional patent medicine, that was commonly used in clinical practice, where the isosceles trapezoid method was first applied for the optimization of background electrolyte solution, and the resolution index was performed to assess the experimental conditions; furthermore, a novel linear quantitative fingerprint method was established for accurate qualitative and quantitative discrimination of the test samples from diverse commercial brands. The fingerprint analysis coupled with quantitative determination of two components was employed to elucidate that the quality consistency of the products was relatively good within one manufactory, but poor among different companies for the 30 batches of samples. In addition, the fingerprint-efficacy relationship between chemical components and antioxidant activity in vitro was investigated using partial least squares analysis, and the calibration and prediction of the antioxidant activity of the selected samples via fingerprint data were presented with the desired results. This work illustrates that the proposed fingerprint analysis based on linear quantitative fingerprint method can be applied for the quality evaluation of traditional Chinese medicine and herbal preparations as part of their quality control, and the constructed mathematical model is particularly suitable for depicting the fingerprint-efficacy relationship.

Total detection of Tianma Toutong tablets for quality consistency by a five‐wavelength fusion fingerprint and chemometrics

“…In vitro evaluation of the antioxidant activity of effective compounds or plant extracts is an important aspect of functional factor research . In this study, DPPH free radical scavenging was used to measure the total antioxidant activity.…”

A Comprehensive Strategy to Quantify the Complex System by Ultraviolet and Infrared Spectra Analyses Coupled with Combustion Heat for Recognizing the Quality Consistency of San-Huang Tablets

“…However, a great difference was observed in quantitative similarity P UV , which made an obvious distinction of all samples. The results in Table 3 shown that most of the samples were qualified (grade ≤ 5) [13] except S8, S12, S18, S28, S30 for their higher contents in the range of 145.5% -253.8%, and S17, S21, S22, S25, S27 for their lower contents in the range of 46.3% -67.5%. Moreover, S1 and S2 from the same manufactory were proved to be accordant in quality of grade 2 and 3, respectively.…”

Qualitative and quantitative analysis of multiple components for quality control of Deng‐Zhan‐Sheng‐Mai capsules by ultra high performance liquid chromatography tandem mass spectrometry method coupled with chemometrics

“…Given the increasing global demand for herbal medicines, QC has become increasingly important in business and clinical applications . Herbal patent medicines contain relatively unrefined mixtures of phytochemical compounds, causing their QC to be highly challenging .…”