Capillary electroendoosmotic chromatography of peptides

Walhagen, Karin; Unger, Klaus K.; Hearn, Milton Thomas William

doi:10.1016/s0021-9673(00)00460-x

Cited by 64 publications

(53 citation statements)

References 67 publications

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“…Capillary electrochromatography (CEC) has also become a powerful separation technique because it provides high column efficiency and selectivity by combining the advantages from both high-performance liquid chromatography (HPLC) and CE [35,36]. In CEC, the flow can be driven by both hydrodynamics and electroosmosis [37,38].…”

Section: Discussionmentioning

confidence: 99%

Photobleaching‐based flow measurement in a commercial capillary electrophoresis chip instrument

Wang¹,

Sas²,

Jiang³

et al. 2008

Electrophoresis

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For microfluidic analytical instruments, a facile, fast, and accurate instrument test is highly demanded. The test includes the quantitative verification of the relationship between pressure drop and flow velocity for the hydrodynamic pump, between the electric voltage and electroosmotic flow (EOF) for the high-voltage supply, and the chip quality. The key point for the test is the measurement of the flow velocity. However, most currently available velocimetries cannot be directly used without any instrumental modification or adding extra instruments. We applied a recently developed Laser Induced Fluorescence Photobleaching Anemometer (LIFPA) for the instrument test through measuring fluid flow velocity in a microfluidic instrument with optical measurement without any modification and extra instrument. We have successfully used the method to test Caliper HTS 250 System from Caliper Life Sciences (Hopkinton, MA) with its own light source and detector. The experimental result demonstrates that this single-point method of measuring flow velocity can be easily used for accurate test of a microfluidic instrument in less than 10 min at extremely low cost without any modification and extra instrument.

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Section: Discussionmentioning

confidence: 99%

Photobleaching‐based flow measurement in a commercial capillary electrophoresis chip instrument

Wang¹,

Sas²,

Jiang³

et al. 2008

Electrophoresis

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“…Capillary electrochromatography (CEC) can also be effective for peptides [21]. Recent work on monolithic systems [22] demonstrates that the somewhat hydrophobic acrylates that are quaternized work well for peptides.…”

Section: Separation Of Neuropeptidesmentioning

confidence: 99%

Techniques for neuropeptide determination

Sandberg

Weber

2003

TrAC Trends in Analytical Chemistry

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Because immunoassay responds to epitopes, and many molecules share the same peptide epitope, it is very difficult to obtain an accurate understanding of peptides, their creation and hydrolysis, in biological systems. Separate-and-detect approaches have merit in that the many active peptides and inactive fragments of a particular system can be separately determined. This review discusses the separation, by chromatography and capillary electrophoresis, and detection, by absorbance, fluorescence, electrochemistry, and immunoassay techniques. When separation pre-concentration is accompanied by laser-induced fluorescence or biuret-based electrochemical detection, nM-pM detection limits are obtained.

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“…However, for CEC to become a widely accepted analytical technique it is necessary to offer a means to separate charged biomolecules with a selectivity different from that obtained in HPLC and capillary zone electrophoresis (CZE). Thus, the focus of recent CEC has concentrated on the separation of biomolecules including amino acids [10][11][12][13] and peptides [14][15][16][17][18] with packed, monolithic, and coated capillary columns. Most of these separations using packed capillary columns can be performed on n-alkyl functionalized silica packing materials (e.g., C 8 , C 18 ) under acidic conditions.…”

Section: Introductionmentioning

confidence: 99%

Investigation of the novel mixed‐mode stationary phase for capillary electrochromatography. II. Separation of amino acids and peptides on sulfonated naphthalimido‐modified silyl silica gel

et al. 2004

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The potential of 3-(4-sulfo-1,8-naphthalimido)propyl-modified silyl silica gel (SNAIP) as a mixed-mode stationary phase for capillary electrochromatography (CEC) was investigated for the separation of charged analytes, taking four amino acids (tyrosine, phenylalanine, tryptophan, histidine) as model analytes. The elution process of these charged analytes in CEC with SNAIP was dominated by a combination of both electrophoretic process and chromatographic process involving hydrophobic as well as electrostatic interactions. In order to study the retention mechanism, the CEC retention factor k* and the velocity factor ke* were measured for the amino acids, which allowed the assessment of the respective contribution from the differential processes underlying the separation. Migration and retention could be mediated by changing various mobile phase compositions, including buffer pH, buffer concentration, and concentration of organic solvent. Based on the results obtained by separation of the amino acids, the separation of eight peptides (Gly-Val, Gly-Phe, Gly-Ile, Gly-His, Gly-Lys, Lys-Lys, Gly-Gly-Gly, Gly-Gly-His) was attempted. A good separation was achieved under an isocratic elution with a mobile phase consisting of 35 mM phosphate buffer (pH 3.8) and 40% methanol.

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Capillary electroendoosmotic chromatography of peptides

Cited by 64 publications

References 67 publications

Photobleaching‐based flow measurement in a commercial capillary electrophoresis chip instrument

Photobleaching‐based flow measurement in a commercial capillary electrophoresis chip instrument

Techniques for neuropeptide determination

Investigation of the novel mixed‐mode stationary phase for capillary electrochromatography. II. Separation of amino acids and peptides on sulfonated naphthalimido‐modified silyl silica gel

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